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A kind of composite adjuvant, the vaccine containing the composite adjuvant and the preparation method of the vaccine

An adjuvant and vaccine technology, applied in the field of immunology, can solve the problems of insignificant immune response effect and high price, and achieve the effect of improving the immune response level, low cost and stable performance.

Active Publication Date: 2020-03-27
INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problems of the existing vaccine adjuvants such as high price and insignificant effect of promoting immune response, the present invention provides an economical, effective, safe and stable compound adjuvant, a vaccine containing the compound adjuvant and the preparation of the vaccine method, the specific scheme is as follows:

Method used

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  • A kind of composite adjuvant, the vaccine containing the composite adjuvant and the preparation method of the vaccine
  • A kind of composite adjuvant, the vaccine containing the composite adjuvant and the preparation method of the vaccine
  • A kind of composite adjuvant, the vaccine containing the composite adjuvant and the preparation method of the vaccine

Examples

Experimental program
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Effect test

Embodiment 1

[0021] The hepatitis A vaccine containing 4-phenylimidazole + aluminum hydroxide compound adjuvant provided in this example is: dissolve 500 μg of 4-phenylimidazole in 3.8 μL of 75% ethanol, and mix it with 300 μg of aluminum hydroxide to obtain a composite adjuvant. Add each single portion of HAV antigen (that is, the single injection dose used in animal experiments) according to the routine, and finally add 1×PBS (PH=7.4) to 300 μL, mix well, and obtain the compound adjuvant containing 4-phenylimidazole + aluminum hydroxide Doses of Hepatitis A Vaccine. In addition, a single adjuvant group (4-phenylimidazole) was set up: after dissolving 1 mg of 4-phenylimidazole in 7 μL of 75% ethanol, add each single dose of HAV antigen (that is, the single injection dose used in animal experiments) as usual, and finally add Add 1×PBS (PH=7.4) to 300 μL, and mix evenly to obtain a hepatitis A vaccine containing 4-phenylimidazole as a single adjuvant.

[0022] Wherein, 4-phenylimidazole is...

Embodiment 2

[0040]The hepatitis A vaccine containing aluminum hydroxide + 4-phenylimidazole compound adjuvant provided in this example is: dissolve 1 mg of 4-phenylimidazole in 7 μL of 75% ethanol and mix it with 300 μg of aluminum hydroxide to obtain a compound adjuvant. Routinely add each single portion of HAV antigen (that is, the single injection dose used in animal experiments), and finally add 1×PBS (PH=7.4) to 300 μL, mix well, and obtain a compound adjuvant containing 4-phenylimidazole + aluminum hydroxide hepatitis A vaccine. In addition, a single adjuvant group (4-phenylimidazole) was set up: after dissolving 1 mg of 4-phenylimidazole in 7 μL of 75% ethanol, add each single dose of HAV antigen (that is, the single injection dose used in animal experiments) as usual, and finally add Add 1×PBS (PH=7.4) to 300 μL, and mix evenly to obtain a hepatitis A vaccine containing 4-phenylimidazole as a single adjuvant. Wherein, 4-phenylimidazole+aluminum hydroxide and HAV antigen are obtai...

Embodiment 3

[0047] The hepatitis A vaccine containing aluminum hydroxide + 4-phenylimidazole compound adjuvant provided in this example is: after dissolving 1.5 mg of 4-phenylimidazole in 10.4 μL of 75% ethanol, mix it with 300 μg of aluminum hydroxide to obtain a compound adjuvant, the compound adjuvant Add each single portion of HAV antigen (that is, the single injection dose used in animal experiments) according to routine, and finally add 1×PBS (PH=7.4) to 300 μL, mix well, and obtain a compound containing 4-phenylimidazole + aluminum hydroxide Adjuvanted hepatitis A vaccine. In addition, a single adjuvant group (4-phenylimidazole) was set up: after dissolving 1 mg of 4-phenylimidazole in 7 μL of 75% ethanol, add each single dose of HAV antigen (that is, the single injection dose used in animal experiments) as usual, and finally add Add 1×PBS (PH=7.4) to 300 μL, and mix evenly to obtain a hepatitis A vaccine containing 4-phenylimidazole as a single adjuvant.

[0048] Wherein, 4-pheny...

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Abstract

The invention relates to a composite adjuvant, a vaccine comprising the same and a preparation method of the vaccine. The composite adjuvant comprises a noncompetitive inhibitor of indoleamine-2,3 dioxygenase and aluminum salts, the noncompetitive inhibitor of the indoleamine-2,3 dioxygenase is 4-benzimidazole, the content of the 4-benzimidazole is 0.5-1.5 mg, the aluminum salts are aluminium hydroxide, and the content of the aluminium hydroxide is 300 mu g. The invention further relates to the vaccine comprising the composite adjuvant and a preparation method of vaccine. The raw materials of the composite adjuvant are easy to obtain, a preparation process of the vaccine is simple, the cost is low, the performance is stable, toxic and side effects are small, immune response is promoted jointly from the positive aspect and the negative aspect of organism immunoregulation, the composite adjuvant is safe and reliable when used in a range of immunizing dose, humoral immune response of antigenic specificity can be induced effectively, humoral immune responses of the antigenic specificity are enhanced remarkably, and the effect of the composite adjuvant is higher than the effects of a group without adjuvant, a group with a single adjuvant and a group with an aluminum adjuvant.

Description

technical field [0001] The invention relates to an adjuvant, in particular to a compound adjuvant for vaccines, and also relates to a vaccine containing the compound adjuvant and a preparation method of the vaccine, belonging to the field of immunology. Background technique [0002] In recent years, with the deepening of vaccine research, especially the rapid development of molecular biology technology, new genetic engineering vaccines have been developed with high purity and strong specificity, but the molecules are small and the immunogenicity is relatively weak, so it is difficult to produce effective vaccines. Therefore, it is particularly important to use adjuvants to enhance its immunogenicity. [0003] Existing vaccine adjuvants have disadvantages such as high price and insufficient effect of enhancing immune response. At present, there are still very few adjuvants that have been certified for use in humans, and aluminum salt adjuvants are one of them. Aluminum salt ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/39A61K39/29A61P31/14
CPCA61K39/12A61K39/39A61K2039/55505A61K2039/55511C12N2770/32434
Inventor 马静王海漩胡凝珠胡云章
Owner INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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