The invention relates to the field of pool coatings, and particularly discloses a monatomic algae removal and sterilization pool coating and a preparation method thereof. The monatomic algae removal and sterilization pool coating comprises the following raw materials in percentage by weight: 20-30% of deionized water, 2.0-3.0% of a dispersing agent, 0.2-1.0% of a defoaming agent, 1.5-2.5% of propylene glycol, 20-35% of a filler, 1.0-2.0% of a thickening agent, 1.0-3.0% of a coalescing agent, 35-45% of superfine styrene-acrylic emulsion, 0.1-0.5% of a pH regulator and 1.0-8.0% of a monatomic algae removal bactericide. The preparation method comprises the steps: firstly, adding the deionized water, adding the dispersing agent, defoaming agent, propylene glycol, filler and monatomic algae removal bactericide while stirring, stirring, grinding, sequentially adding the thickener, deionized water, coalescing agent, superfine styrene-acrylic emulsion, defoaming agent and pH regulator, and stirring to obtain the finished product. The coating is applied to a pool coating, and has the advantages of algae removal, algae prevention, antibiosis, disinfection and lasting purification.