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Metal aluminum nano adjuvant, preparation method thereof, vaccine composition, preparation method of vaccine composition and application of metal aluminum nano particle

A vaccine composition, metal aluminum technology, applied in the directions of vaccines, drug combinations, DNA/RNA vaccination, etc., can solve the problems of low induction efficiency, and achieve the enhancement of humoral immune response, enhancement of cellular immune response, stability and safety. Good results

Pending Publication Date: 2021-04-27
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The technical problem to be solved by the present invention is to overcome the defects of low cellular immune response induction efficiency of traditional aluminum salt adjuvants (such as aluminum hydroxide, aluminum phosphate, etc.), and provide a metal aluminum nano adjuvant, vaccine composition and its preparation Methods and Applications

Method used

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  • Metal aluminum nano adjuvant, preparation method thereof, vaccine composition, preparation method of vaccine composition and application of metal aluminum nano particle
  • Metal aluminum nano adjuvant, preparation method thereof, vaccine composition, preparation method of vaccine composition and application of metal aluminum nano particle
  • Metal aluminum nano adjuvant, preparation method thereof, vaccine composition, preparation method of vaccine composition and application of metal aluminum nano particle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0117] Example 1 Preparation of metal aluminum nanoparticles by electric explosion method

[0118]In an argon environment, fix an aluminum wire with a diameter of 0.2mm and a purity greater than or equal to 95% between high-voltage electrodes, and use a mechanical device to continuously supply power. The power supply capacitance is 96μF, and the electrode discharge voltage is 4.0-4.4kV. Under high-density current, the aluminum wire is heated and radially expanded to vaporize the aluminum wire to form aluminum vapor, which is then condensed to form aluminum particles with an average particle size of 139.76±42.81nm, which is carried out in a collector filled with argon. collect. (See literature: Passivation Process for Superfine Aluminum Powders Obtained by Electrical Explosion of Wires. Appl. Sur. Sci. 2003, 211, 57–67.)

Embodiment 2

[0119] Example 2 Preparation of metal aluminum nanoparticles by ligand regulation method

[0120] (1) Precursor H 3 Synthesis and Characterization of Al(1-MP)

[0121] In a glove box (with both oxygen and water content below 1 ppm), 3.748 g of lithium aluminum hydride and 4.132 g of aluminum chloride were added to a flask containing 45 mL of anhydrous toluene. Under vigorous stirring (stirring speed: 800 rpm), 11.65 mL of 1-methylpyrrolidine (1-methylpyrrolidine) was added dropwise. Wherein, the molar ratio of lithium aluminum hydride, aluminum chloride, and 1-methylpyrrolidine is 3:1:1. After overnight reaction at room temperature, the reaction solution was filtered through a funnel to remove solid impurities. For further purification, the obtained filtrate was filtered again with an organic phase filter membrane with a pore size of 0.22 μm, and the obtained filtrate was H 3 Al(1-MP) (structural formula is Yield is 90%) in toluene solution, and low-temperature preservat...

Embodiment 3

[0136] Vaccine AlNPs-OVA was prepared by mixing electroexplosive metallic aluminum nanoparticles with OVA. Specific steps are as follows:

[0137] (1) The electroexplosive metal aluminum nanoparticles prepared in Example 1 are made into a solution of 0.1-5.0 mg / mL (using DMF as a solvent). minute.

[0138] (2) Prepare ovalbumin OVA PBS solution with a concentration of 0.1-5.0 mg / mL. In this implementation, the concentration of ovalbumin OVA is 3.0 mg / mL.

[0139] (3) Mix the PBS solution of ovalbumin OVA and the DMF solution of nano-aluminum particles according to the volume of 1:1, ultrasonically disperse for half an hour, transfer to a shaker, and shake at room temperature at 320rpm for 12 hours. After stopping the shaking, centrifuge at 12000rpm for 10 minutes, remove the supernatant and freeze-dry the sample to remove the remaining small amount of solvent.

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Abstract

The invention discloses a metal aluminum nano adjuvant, a preparation method thereof, a vaccine composition of the vaccine composition and an application of a metal aluminum nano particle. The vaccine adjuvant comprises the metal aluminum nano particle, and can be used as a candidate adjuvant of preventive vaccines and therapeutic vaccines for various diseases such as infection, autoimmune diseases and tumors. The vaccine adjuvant provided by the invention is combined with an antigen for use, so that humoral immune response and cellular immune response of the vaccine can be effectively enhanced, and the enhancement effect of the vaccine adjuvant is remarkably superior to that of a commercially available aluminum hydroxide adjuvant.

Description

technical field [0001] The invention relates to the field of vaccine preparations, in particular to a metal aluminum nano adjuvant, a vaccine composition and a preparation method and application thereof. Background technique [0002] Vaccines are usually used to prevent infectious diseases such as influenza, such as the classic diphtheria vaccine and commonly used influenza vaccine, etc. In recent years, vaccine research and development is moving from prevention to treatment. In addition to treating tumors, the development of therapeutic vaccines also includes vaccines for lifestyle-related diseases such as allergies, diabetes, and hypertension. Therapeutic vaccines appear in various forms, such as short peptide or protein vaccines, RNA or DNA vaccines, cancer cell vaccines, etc. Although clinical trials have been conducted for several years, most of them have failed to prove effective anticancer effects. One of the important reasons for the ineffectiveness of therapeutic v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K9/19A61K39/00A61P35/00
CPCA61K39/39A61K9/19A61K39/0011A61P35/00A61K2039/55505A61K2039/53
Inventor 刘堃于华杨成功孙天盟王野朱歌
Owner JILIN UNIV
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