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Method for detecting anti-inflammatory activity of rhododendron molle extractive and application thereof

A technology of extracts of roe deer, which is applied in the field of extracts of roe deer, can solve the problems of limiting clinical application development and unclear anti-inflammatory mechanism, and achieves the effect of huge scientific research value, sensitive cell detection model, and simple extraction process

Pending Publication Date: 2019-01-04
JIANGXI NORMAL UNIV
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Problems solved by technology

From the above clinical reports, it can be seen that as a unilateral treatment of rheumatoid arthritis, the sheep roe deer has a significant effect on the treatment of rheumatoid arthritis and has important development prospects. However, its anti-inflammatory mechanism is still unclear, which limits its clinical application and further development.

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  • Method for detecting anti-inflammatory activity of rhododendron molle extractive and application thereof
  • Method for detecting anti-inflammatory activity of rhododendron molle extractive and application thereof
  • Method for detecting anti-inflammatory activity of rhododendron molle extractive and application thereof

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Embodiment Construction

[0020] The beneficial effects of the present invention will be described in detail below in conjunction with the embodiments of the accompanying drawings, aiming at helping readers better understand the essence of the present invention, but not limiting the implementation and protection scope of the present invention.

[0021] (1) Preparation of methanol extract of Rhododendron molle G. Don: leaves of Rhododendron molle G. Don, the experimental material, were collected in Hunan Province, China in July. Wash the leaves of a fresh wild goosebump collected in the field, place them in a freeze dryer to dry, take out the leaves after 48 hours, grind the leaves into powder with a powder machine and pass through an 80-mesh sieve. Soak 10 g of the powder of the leaves of A. chinensis in methanol solution (1:10, W / V) overnight at room temperature, and then reflux extraction three times, each time for 1.5 hours. Then the methanol extract was filtered through a 0.45 μm filter membrane, a...

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Abstract

The invention relates to a method for detecting anti-inflammatory activity of a rhododendron molle extractive and application thereof. The method comprises the steps that a preparation method of the rhododendron molle extractive is established, a rhododendron molle extractive anti-inflammatory activity cell detection model is constructed, and an analysis method of the rhododendron molle extractivefor inhibiting inflammatory factors and related gene expression in inflammatory cells is established. The method for detecting the anti-inflammatory activity of the rhododendron molle extractive andthe application thereof have the advantages that results show that a rhododendron molle blade methanol extractive has stronger anti-inflammatory activity, can significantly inhibit secretion of nitricoxide (NO), tumor necrosis factor-alpha (TNF-alpha), interleukin 1beta (IL-1belta) and other inflammatory factors in RAW264.7 cells stimulated by lipopolysaccharide (LPS) and gene expression thereof,and also significantly inhibit the expression of two key enzyme genes of iNOS and COX-2 in relevant paths of inflammation.

Description

technical field [0001] The invention relates to extracts of roe deer, in particular to a method for detecting the anti-inflammatory activity of the extract of roe deer and a method for using the extract of roe deer for anti-inflammation. Background technique [0002] Inflammation refers to a complex physiological and pathological reaction mainly based on defense response caused by physical damage, bacterial infection and other harmful stimuli. It is a defense mechanism of the body, mainly manifested as redness, heat, swelling, pain Even clinical symptoms such as dysfunction. Rheumatoid arthritis (RA), chronic bronchitis, chronic gastritis, inflammatory bowel disease, atherosclerosis, senile dementia and neurodegenerative diseases and other diseases are accompanied by inflammatory reactions, so whether the inflammation can be effectively controlled Development plays an important role in the treatment of diseases. Existing anti-inflammatory drugs are mainly divided into carr...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50A61K36/45A61P29/00A61P19/02
CPCA61K36/45A61K2236/13A61K2236/17A61K2236/33A61K2236/39A61K2236/51A61P19/02A61P29/00G01N33/5008G01N33/5014
Inventor 罗向东张金戴亮芳刘见董丽敏谢建坤
Owner JIANGXI NORMAL UNIV
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