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Method for measuring specificity HIV antibody generated by human immunodeficiency virus (1+2 type) different antigen epitope

A technology for human immunodeficiency and antigenic epitopes, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of limiting the application of confirmation reagents, unaffordable detection costs, and specificity limitations, so as to reduce non-specific reactions and improve sensitivity and sensitivity, the effect of improving stability

Active Publication Date: 2007-12-05
AUTOBIO DIAGNOSTICS CO LTD
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Problems solved by technology

These imported reagents are expensive, and the vast majority of infected people in China are concentrated in rural or underdeveloped areas, unable to afford expensive testing costs
This situation greatly limits the application of confirmatory reagents in AIDS testing in my country
Only a few research institutions in China have carried out the research and development of immunoblot diagnostic reagents for AIDS. The only domestic confirmation reagent uses whole virus as the antigen, and its specificity is limited. At the same time, the complicated process also makes the cost of the reagent high. The price is not much different from that of imported products, so it cannot be promoted and used in China

Method used

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  • Method for measuring specificity HIV antibody generated by human immunodeficiency virus (1+2 type) different antigen epitope
  • Method for measuring specificity HIV antibody generated by human immunodeficiency virus (1+2 type) different antigen epitope

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Embodiment Construction

[0018] Antigen strips for measuring specific HIV antibodies produced by different antigenic epitopes of human immunodeficiency virus (type 1+2), including a bottom plate 1 made of PVC, and a nitrocellulose membrane 2 coated with HIV specific antigens is arranged in the middle of the bottom plate 1 A colloidal gold pad 3 made of a polyester film coated with goat anti-human IgG colloidal gold-labeled antibody is superimposed on the lower part of the nitrocellulose membrane 2, a sample pad 4 made of glass fiber is superimposed on the colloidal gold pad 3, and a nitrocellulose Paste the cotton pulp board 5 that attracts sample above plain film 2 top.

[0019] The specific HIV gene recombinant antigens include:

[0020] The gp41, gp120 and gp160 gene recombinant antigen fragments of the HIV-1 Env band, the p55 and p24 gene recombinant antigen fragments of the Gag band, the p51 and p31 gene recombinant antigen fragments of the Pol band, and the HIV-2 gp36 gene recombinant antigen fr...

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Abstract

The invention discloses a generating method of astopic HIV antibody at different antigen surfaces of human immune defect (1+2 type) to judge whether the person is infected by HIV and which hypotype of HIV, which comprises the following steps: preparing astopic antigen strip of HIV antibody on the nitrate cellulose film covered by recombinant antigen of astopic HIV gene; adopting colloidal gold to mark goat-anti-human lgG as signal trace system; reacting the HIV astopic antibody in the sample and colloidal gold goat-anti-human lgG to do corresponding astopic reaction; form antibody sandwich compound of astopic HIV antigen-astopic HIV antibody-colloidal gold marked goat-anti-human lgG; judging whether existing the astopic antibody through special color trace of colloidal gold particle. The inveniton improves the specificity and stabilizes the property of signal trace system with visual judging result, which avoids non-specific and false dismissal probability.

Description

technical field [0001] This product relates to the field of in vitro immunodiagnosis, in particular to a method for measuring specific HIV antibodies produced by different antigenic epitopes of human immunodeficiency virus (type 1+2), and judging whether the subject is infected with HIV or not Which subtype of HIV. Background technique [0002] AIDS (AIDS), also known as acquired immunodeficiency syndrome (acquiredimmuno-deficiency syndrome), is a serious infectious disease caused by human immunodeficiency virus (Human ImmuneDeficiency Virus, HIV). It is a chronic infectious disease characterized by opportunistic infections and malignant tumors, which can damage the human immune system, especially the cellular immune system, through sexual contact and body fluid transmission. AIDS is an infectious disease with no effective cure and high fatality rate. Its widespread prevalence in the world has become a serious public health and social problem. [0003] At present, American...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/558G01N33/549G01N33/577
Inventor 付光宇李桂林刘功成李彬项立红陆莹马建军吴学炜
Owner AUTOBIO DIAGNOSTICS CO LTD
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