Chemiluminescent kit for detecting EGFR

Abstract

The invention discloses a chemiluminescent kit for detecting an EGFR. The chemiluminescent kit comprises a kit body and a reagent bottle in the kit body; the kit body comprises the kit body and a kitcover; the kit cover covers the kit body; the kit body is made from a PMMA material, is firm and durable, and is not easily damaged in a transportation process; a first gap is formed in the upper surface of the kit cover; an anti-skid pad is arranged on the lower surface of the kit body; the anti-skid pad is matched with the first gap; a plurality of kits are stacked together, so that the space issaved, and the transportation is facilitated; a damping pad and a fixing pad are arranged, so that the functions of fixing and protecting the reagent bottle can be achieved; a horseradish peroxidaselabeled EGFR antibody, an EGFR antibody coating a micropore plate and a to-be-detected sample form a "solid-phase coated antibody-to-be-detected antigen-enzyme labeled antibody" sandwich compound structure; a horseradish peroxidase is used for catalyzing a luminescent substrate, namely, luminol, so that the sensitivity is greatly improved; and a more specific, rapid and reliable basis can be provided for clinical diagnosis.

Description

technical field [0001] The invention relates to the field of medical immunological in vitro diagnostic reagents, in particular to a chemiluminescence kit for detecting EGFR. Background technique [0002] Epidermal Growth Factor Receptor (EGFR) is a receptor for epidermal growth factor (EGF) cell proliferation and signal transduction. EGFR is a glycoprotein, which belongs to the tyrosine kinase type receptor, and the cell membrane runs through, with a molecular weight of 170kDa. In the process of scientific research and production, it is necessary to measure the change of EGFR protein content in the solution to monitor the experimental conditions and monitor the production process. EGFR is widely distributed on the cell surface of mammalian epithelial cells, fibroblasts, glial cells, keratinocytes, etc., and the EGFR signaling pathway plays an important role in physiological processes such as cell growth, proliferation, and differentiation. The loss of function of protein t...

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Problems solved by technology

[0003] At present, EGFR assay methods include colloidal gold immunochromatography (GICA); Western blot (WB); enzyme-linked immunosorbent assay (ELISA), chemiluminescence immunoassay (CLIA); the advantages of GICA, ELISA, and WB are relatively low cost, The disadvantage is that the sensitivity is low, and it cannot be detected when the EGFR protein content is small. As a non-radioactive immunoassay method, CLIA has the characteristics of high sensitivity, short time required, no pollution, and wide detection range. The advent of instruments and the continuous introduction of different types of chemiluminescent immunoassay kits have become the substitutes for time-resolved, radioimmunoassay, and enzyme-linked immunoassays. However, there are still few chemiluminescent kits for EGFR on the market

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