DNA (Deoxyribonucleic Acid) low-temperature storage solvent and storage method thereof

Abstract

The invention discloses a DNA (Deoxyribonucleic Acid) low-temperature storage solvent and a storage method thereof. The DNA storage solvent comprises calf serum, glycerol, a TE buffer solution, dimethyl sulfoxide and glycine betaine, wherein the final volume of the calf serum is 0.1%-1%, the final volume of glycerol is 10%-24%, the final concentration of the dimethyl sulfoxide is 30%-50%, the final concentration of the glycine betaine is 1M-7M and the pH range of the DNA storage solvent is 6.0-8.0. The DNA storage method comprises the following steps of: (1), extracting DNA; and (2), storing the DNA in the DNA storage solvent for storage. According to the DNA low-temperature storage solvent and the storage method thereof disclosed by the invention, the operation is simple, the cost is low, the DNA storage effect is good, the storage period is long and multigelation can be avoided, so that the most perfect DNA is provided for the scientific research, the identity authentication and the medical diagnosis.

Description

technical field [0001] The invention relates to the preservation of DNA (deoxyribonucleic acid, deoxyribonucleic acid), in particular to a DNA cryogenic preservation solvent and a preservation method thereof. Background technique [0002] DNA is an important component of chromosomes and is the carrier of genetic information. It contains the genetic information of individual species and is the most important biological information molecule. It can be used to construct genomic libraries, isolate required genes, and detect related molecular markers. Genomic DNA is not tissue specific, that is, the DNA is the same regardless of the biological tissue it is made from. Due to the large amount of genetic information contained in DNA, easy access and extremely stable chemical properties, DNA is one of the main contents of the collection and preservation of germplasm resources and genetic resources. [0003] The sequence structure of DNA molecules has three important characteristics:...

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Problems solved by technology

[0005] In order to maintain the integrity of the DNA structure and avoid large-scale DNA fragmentation and degradation, the best way to preserve DNA is to store it in dry powder at -80°C or at low temperature in liquid nitrogen. However, a large amount of DNA is required to make dry powder, and it is difficult to obtain materials. Not operational in resource collection

However, using absolute ethanol to preserve DNA, although it can be stored in advance, there is also the problem of repeated freezing and thawing.

The solid-phase DNA preservation technology is a new concept recently proposed, which is suitable for permanent preservation, and whether the preservation matrix of this method affects the subsequent DNA experiments and the specific effect of preservation, there is currently no data in this regard, and it remains to be tested by time

In addition, for samples that need to be used multiple times during storage, DNA needs to be re-extracted, which is complicated and can easily lead to DNA breakage, which will make it impossible to perform some detection experiments that require high DNA quality

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