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Biobank tissue cryopreservation methods and biobank tissue cryopreservation tubes

A technology for biological samples and cryopreservation methods, which is applied in the preservation, transportation and packaging of human or animal bodies, special packaging objects, etc., can solve problems such as difficult identification and removal, and achieve the effect of reducing damage

Active Publication Date: 2018-02-16
FUDAN UNIV SHANGHAI CANCER CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the above problems, the main purpose of the present invention is to provide a method for cryopreservation of biological sample bank tissue, which can solve the problems caused by the adhesion of biological sample bank tissue on the inner wall of the cryopreservation tube after freezing. It is not easy to be identified or taken out. On the other hand, it can avoid the repeated freezing and thawing of biobank tissues when making frozen sections, which is beneficial to the protection of the quality of tissue samples.

Method used

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  • Biobank tissue cryopreservation methods and biobank tissue cryopreservation tubes
  • Biobank tissue cryopreservation methods and biobank tissue cryopreservation tubes
  • Biobank tissue cryopreservation methods and biobank tissue cryopreservation tubes

Examples

Experimental program
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Effect test

Embodiment 1

[0045] A method for freezing of biological sample bank tissue, comprising the steps of:

[0046] (1), separating the required biological tissue from the living body;

[0047] (2), processing the freshly separated biological tissue into a suitable size, placing it on the carrier sheet as a biological sample bank tissue, so that the biological sample bank tissue adheres to the surface of the carrier sheet;

[0048] (3), placing the carrier sheet adhered to the biological sample bank tissue in the biological sample bank tissue cryopreservation tube;

[0049] (4) Seal the tissue cryopreservation tube of the biological sample bank, and place it in a suitable direction at a low temperature for cryopreservation.

[0050]Wherein, in step (1), the biological tissue can be various human tissues or animal tissues, including surgically removed tissues and experimental animal tissues. According to the needs of the experiment, these biological tissues can be tumor tissues and normal tissu...

Embodiment 2

[0056] Such as figure 1 As shown, this embodiment provides a tissue cryopreservation tube 1 for a biological sample bank, which includes: a tube body 2 , a tube cap 3 and a carrier sheet 4 .

[0057] The tube body 2 is in the shape of a hollow cylinder, and is detachably connected with the tube cover 3 . When the tube body 2 and the tube cover 3 are connected together, the two form a closed space to prevent external substances (such as cryopreservation liquid, etc.) from entering the space during freezing, thereby avoiding the space from being polluted.

[0058] The tube body 2 and the tube cover 3 can be connected by various connection methods such as threaded connection and buckle connection. If the pipe body 2 and the pipe cover 3 are threaded to form a sealed connection, then an external thread can be established on the outer surface of the pipe body 2, and an internal thread can be established on the inner surface of the pipe cover 3, and the pipe body 2 and the pipe cov...

Embodiment 3

[0069] In the biological sample bank tissue cryopreservation tube in Example 2, only one biological sample bank tissue can be frozen in each tube. On the one hand, when a large number of biobank tissues need to be cryopreserved, an equal number of biobank tissue cryopreservation tubes are required, which may increase the cost of cryopreservation; During cryosectioning, it is necessary to continuously open the tissue cryopreservation tubes of the biological sample bank to take out the frozen tissues, which is time-consuming and laborious, which is not conducive to improving the efficiency of cryosectioning.

[0070] In order to solve the above problems, the tissue cryopreservation tube of the biological sample bank in this embodiment has been improved in structure, so that one biological sample bank tissue cryopreservation tube can freeze multiple tissues at the same time, so that when making frozen sections, just close the Or open the cryopreservation tube of the biobank tissu...

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Abstract

The invention provides a biological sample bank tissue cryopreservation method and a biological sample bank tissue cryopreservation tube. The freezing method comprises: placing the biological sample bank tissue on the surface of a carrier sheet; The slices are placed in the tissue cryopreservation tube of the biobank, and the tissue cryopreservation tube of the biobank is sealed and stored at a low temperature; the cryopreservation tube includes a tube body, a cap and at least one for holding the separated biobank tissue. The carrier sheet can be detachably placed in the tube body; this cryopreservation method can solve the problem that the tissue of the biological sample bank is not easy to be identified or taken out because it is pasted on the inner wall of the cryopreservation tube after cryopreservation. It can also avoid repeated freezing and thawing of biological sample bank tissue when making frozen sections, thereby benefiting the protection of biological sample bank tissue quality (for example, biological macromolecules such as RNA, protein, antigen and antibody contained).

Description

technical field [0001] The invention belongs to the technical field of medical devices, and relates to a biological tissue cryopreservation method and a biological tissue cryopreservation tube. Background technique [0002] Biobank is a biological application system that centrally preserves various tissue materials of organisms and uses them for clinical treatment of diseases and life science research. Biobanks include tissue banks (such as normal tissues, tumor tissues, etc.), organ banks, cell banks, etc. [0003] In order to conduct in-depth research on the structural or biochemical properties of biobank tissues, long-term cryopreservation (such as storage in liquid nitrogen) is often required for biobank tissues, and they can be re-frozen at different times after cryopreservation. Conduct research on these biological tissues. Cryotubes are commonly used today for cryopreservation of biobank tissue. [0004] In order to make the cryopreservation tube not only maintain ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02B65D81/18
Inventor 杜祥
Owner FUDAN UNIV SHANGHAI CANCER CENT
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