Preparation method of nucleic acid primer preserving fluid

Abstract

The invention discloses a preparation method of a nucleic acid primer preserving fluid, which is characterized in that the preserving fluid is prepared from the following components in 1000 mL: 2 to 3 parts of thiomersalate and 0.2 to 0.4 part of 1MTris-HCL; 0.1 to 0.3 part of a 0.5 M metal chelating agent; an acidity regulator and a buffer solution; and the balance of pure water. The preparation method comprises the following steps: fully mixing tris (hydroxymethyl) aminomethane, disodium ethylene diamine tetraacetate and pure water until the mixture is completely dissolved to form a first mixture; adding an acid-base regulator into the first mixture obtained in the step 1, mixing, regulating the pH value to 8.0, then adding thiomersalate, and finally adding pure water to a constant volume of 100ml to obtain the nucleic acid preserving fluid. The nucleic acid preservation solution can improve the stability of nucleic acid in a solution and delay degradation of the nucleic acid, so that the preservation period of the nucleic acid in a liquid state can be prolonged. Meanwhile, repeated freeze-dissolution can be effectively avoided, the experiment operation steps are reduced, and the whole experiment process is shortened.

Description

technical field [0001] The invention belongs to the technical field of nucleic acid preservation, and relates to a preparation method of a preservation solution, in particular to a preparation method of a nucleic acid primer preservation solution. Background technique [0002] Nucleic acid is a biomacromolecular compound synthesized by many nucleotides. It exists widely in all animal and plant cells and microorganisms. Nucleic acid in organisms is often combined with protein to form nucleoprotein. According to different chemical composition, nucleic acid can be divided into ribonucleic acid (referred to as RNA) and deoxyribonucleic acid (abbreviated as DNA). The properties of nucleic acids are unstable and easily denatured. Therefore, during the research process, nucleic acids are usually stored at ultra-low temperatures (-70°C) for long-term storage. However, this storage method is likely to affect the structure of nucleic acids, and the research process requires Repeated ...

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Problems solved by technology

[0005] The purpose of the present invention is to solve the unstable performance and easy denaturation of nucleic acids. Therefore, in the research process, nucleic acids are usually stored at ultra-low temperatures (-70°C) for long-term storage. However, this storage method is prone to damage to the structure of nucleic acids. impact, and repeated freezing and thawing are required during the research process; at present, TE solution is mainly used to dissolve nucleic acids, and ultrapure water is used as the solvent

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