The invention relates to a
nucleic acid amplification and chromatographic detection kit based on
ureaplasma urealyticum in a totally-enclosed card box and application of the kit, the kit is a kit based on an RPA isothermal amplification
chromatography technology, a freeze-
drying ball
reagent, a liquid
reagent and a
nucleic acid detection chromatographic test strip are arranged in the kit, the amplification mode is RPA isothermal amplification, and the detection sensitivity is high. The detection mode is a chromatographic test strip, the 5'end of a probe of the
ureaplasma urealyticum RPA is marked with
digoxin, and the 5 'end of a downstream primer is marked with
biotin. The 5'end of the RPA probe of the
internal standard substance bacillus is marked with FAM, and the 5 'end of the downstream primer is marked with
biotin. Two indexes of UU and
reference genes can be simultaneously amplified in the same tube through an RPA isothermal amplification method, an amplification product is double-stranded
DNA with molecular markers at two ends, compared with
fluorescent PCR, the kit integrates
nucleic acid extraction, amplification and detection, the nucleic acid extraction, amplification and detection are all carried out in a card box in a sealed mode, a sample adding step is not needed, operation is simple,
pollution can be effectively prevented, and the kit is suitable for large-scale popularization and application. And
automation of the whole process is realized.