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Coxsackie virus type a16 (ca16) igm antibody detection test strip

A technology for testing test paper and test strips, which is applied to measuring devices, instruments, scientific instruments, etc., to achieve the effects of easy promotion, clear and easy to distinguish results, and saving manpower and material resources

Active Publication Date: 2011-11-30
辽宁迪浩生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, so far, there is no report on a test strip that can detect CA16-specific IgM antibodies conveniently and quickly.

Method used

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  • Coxsackie virus type a16 (ca16) igm antibody detection test strip
  • Coxsackie virus type a16 (ca16) igm antibody detection test strip
  • Coxsackie virus type a16 (ca16) igm antibody detection test strip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: CA16 specific IgM antibody detection test strip (colloidal gold method) (see Figure 1)

[0029] Coxsackie virus A16 type VP1 antigen was purchased from Beijing Amber Gene Technology Co., Ltd.

[0030] The reaction support is a 6.5cm×0.4cm PCV board; the absorbent pad is a 2cm×0.4cm oil filter paper; a 2.0cm×0.4cm nitrocellulose membrane is sequentially coated with anti-mouse IgG (2mg / ml), CA16 antigen VP1 (2.3mg / ml), containing 0.4cm×0.4cm colloidal gold labeled anti-human IgM (labeled amount 25μg / ml) monoclonal antibody polyester film; sample pad 2.7cm×0.4cm glass fiber; which constitutes the Coxsackie virus A16 type (CA16) IgM antibody detection test strip (colloidal gold method).

Embodiment 2

[0031] Example 2 Detection method (see figure 2 )

[0032] Drop 100-150 μl of the patient's whole blood, plasma or serum sample directly into the test strip "4" of Example 1, and the sample solution will go up the membrane, and the result will be interpreted in 10-15 minutes.

[0033] result:

[0034] If the test sample contains the IgM antibody of CA16, it will form a corresponding complex with the anti-human IgM monoclonal antibody labeled with colloidal gold on the test strip, which will be combined with the CA16 specific antigen VP1 coated on the nitrocellulose membrane to form The red line, that is, a red band is formed at T.

[0035] Regardless of whether the specimen contains the corresponding antibody or not, the colloidal gold-labeled anti-human IgM monoclonal antibody continues to crawl upward and forms a red precipitation line with the anti-mouse IgG coated on the membrane, that is, a red band is formed at "C". This line is a quality control line. If colloidal gold fails,...

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Abstract

The invention provides a detection test strip for a coxsackie virus A16 type (CA16) IgM (Immune Globulin M) antibody. According to the detection test strip, a nitrocellulose membrane (NC membrane) is coated by a CA16 gene antigen (VP1) and an anti-mouse IgG; and by combining a tihuman IgM monoclonal antibody labeled by colloidal gold, a CA16 specificity IgM antibody in an infected human speciment is detected by applying a membrane chromatography capture method. The test strip for detection, which is disclosed by the invention, has the advantages of simpleness, convenience, quickness and fastness in operation, no need of special instrument or special training, distinct and easily-distinguished result and easiness for popularization; and the detection test strip is suitable for field detection and early-stage diagnosis and assisting function for CA16 infected diagnosis.

Description

Technical field [0001] The invention belongs to the field of biological detection, and specifically relates to a test strip for detecting Coxsackie virus A16 type (CA16) IgM antibody and its application. Background technique [0002] Coxsackievirus is a serious pathogen of human diseases. Its infection can cause many diseases, from mild respiratory infections to more serious myocarditis, pericarditis and some diseases of the nervous system. Cause death of infants and young children. CoxV can be divided into two groups: A and B: group A has 24 serotypes, and group B has 6 serotypes. All types of group B and type 9 of group A have common group-specific antigens; group B has cross-reactions among virus serotypes, but group A does not. The majority of CoxV that infect humans is group A, and the CoxV that causes hand-foot-mouth disease is mostly type A16, A4, A5, A7, A10, B2, B5, and B13. CoxV is a single-stranded positive-stranded RNA virus with a molecular weight of (2~2.8)×106, ...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/532G01N33/569
Inventor 刘明
Owner 辽宁迪浩生物科技有限公司
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