Dengue fever NS1 antigen and IgG/IgM antibody two-joint inspection test strip, detection card and kit

Abstract

The invention relates to a dengue fever NS1 antigen and IgG / IgM antibody two-joint inspection test strip, which comprises a dengue fever NS1 antigen test strip and a dengue fever IgG / IgM antibody teststrip, and is characterized in that the dengue fever NS1 antigen test strip comprises a first gold labeled pad layer and a first nitrocellulose membrane, and the first gold labeled pad layer is immobilized with a dengue fever NS1 monoclonal antibody colloidal gold compound; and the first nitrocellulose membrane is coated with detection lines of four serotypes of dengue fever NS1 monoclonal antibodies, the dengue fever IgG / IgM antibody test strip comprises a second gold labeled pad layer and a second nitrocellulose membrane, and the second gold labeled pad layer is immobilized with four serotypes of dengue fever recombinant antigen colloidal gold compounds. The second nitrocellulose membrane is coated with a detection line T1 of a mouse anti-human IgG monoclonal antibody and a detection line T2 of a mouse anti-human IgM monoclonal antibody; and according to the test strip, IgM / IgG antibodies and NS1 antigens can be jointly detected through one-time operation, the operation process is simplified, the immune response reaction state of an organism is effectively diagnosed, and the purpose of real POCT detection is achieved.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to a double detection test strip, a detection card and a kit for detecting dengue virus IgG / IgM antibodies and NS1 antigens in serum, plasma and whole blood by using immunochromatography technology. Background technique [0002] Dengue fever is caused by dengue virus and is an acute infectious disease transmitted by Aedes aegypti or Aedes albopictus. Dengue virus is a small flavivirus, which belongs to the Flavivirus genus (Flavivirus) of the family Flaviviridae. According to the epidemiological classification, it belongs to group B of arboviruses and is divided into four serotypes serologically. Dengue virus can cause a series of clinical symptoms. Clinically, the main symptoms are sudden high fever, extensive hemorrhage, severe muscle pain and arthralgia, and extreme fatigue, often accompanied by rash, lymphadenopathy, and leukopenia. [0003] At present, the virus...

AI Technical Summary

Problems solved by technology

ELISA method and PCR method, these detection techniques require special instruments and equipment, and require professional technicians to conduct detection in professional laboratories, and the operation is cumbersome, technically difficult, time-consuming, experimental conditions are high, and there are serious biosafety It is not suitable for real-time on-site detection and is suitable as a confirmation method, but it cannot be used for large-scale primary screening and should be popularized and applied in actual work.