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AB/Rh blood type grouping reagent card and preparation method thereof

A reagent card, rh blood type technology, applied in the field of blood transfusion medicine, can solve the problems of increasing the clinical workload, increasing the production cost of blood type cards, etc., and achieves the effect of strong buffering capacity

Inactive Publication Date: 2012-01-25
JIANGSU ZHONGSHENG MEDICAL DIAGNOSTIC REAGENT
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The existing ABO / Rh reagent card includes ABO positive and negative stereotyping detection reagent cards, but for some patients who know their own blood type, in order to further confirm the blood type before blood transfusion, if they continue to use ABO / Rh positive and negative stereotyping detection reagent cards, not only Increased clinical workload, but also increased blood type card production costs

Method used

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  • AB/Rh blood type grouping reagent card and preparation method thereof

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Embodiment Construction

[0039] A preparation method for an AB / Rh blood type confirmation reagent card, comprising the following steps:

[0040] 1) Preparation of gel buffer

[0041] The buffer formula is as follows:

[0042] 20 mL of 0.15M phosphate buffered saline

[0043] Sodium chloride 1.75g

[0044] Glycine 18g

[0045] Wherein 20mL of 0.15M phosphate buffer was mixed with 11.3mL of 0.15M KH 2 PO 4 and 8.7 mL of 0.15M Na 2 HPO 4 composition;

[0046]Dissolve the above reagents in 800 ml of distilled water, adjust the pH to 6.7 with 1M sodium hydroxide, and finally adjust the volume to 1 liter; add 0.6 g of methyl p-hydroxybenzoate and 0.13 g of propyl p-hydroxybenzoate, and add bovine serum albumin , the added amount is to make the final concentration of bovine serum albumin be 3%; obtain the gel suspension buffer;

[0047] 2) Gel preparation

[0048] Select a dextran gel with a particle size of 20-50 microns, soak it with the gel suspension buffer prepared in step 1), and then wash it...

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Abstract

The invention discloses an AB / Rh blood type grouping reagent card and a preparation method thereof. The reagent card is provided with at least one group of microcolumn gel tubes; each group of microcolumn gel tubes comprise four microcolumn gel tubes, namely a gel tube containing an immune globulin (IgM) monoclonal A-resistant antibody, a gel tube containing an IgM monoclonal B-resistant antibody, a gel tube containing an IgM monoclonal D-resistant antibody and a gel tube which is used for negative control and contains a gel buffer solution. The invention provides standard for the ABO / Rh(D) blood type grouping; and the ABO / Rh(D) blood type grouping reagent card which conforms to standard can be used in all hospitals and blood transfusion services to accurately group ABO / Rh(D) blood types, so conditions are created for guaranteeing transfusion safety.

Description

technical field [0001] The invention relates to the preparation and application of a blood type confirming reagent card. More specifically, the present invention relates to the composition, preparation method and application of an ABO / Rh(D) blood type confirmation reagent card, and relates to the field of transfusion medicine. Background technique [0002] Correct identification of blood type is the first step in safe blood transfusion, and blood transfusion is an important medical method to save patients' lives. Since the discovery of ABO blood type in 1900, Hoktoen first proposed the importance of blood type identification in blood transfusion therapy as early as 1907. In 1908, Ottenberg and others further proposed the concept of cross-match experiment, emphasizing the far-reaching significance of blood group identification for clinical blood transfusion. ABO blood type incompatibility can lead to severe immediate hemolytic transfusion reaction in clinical blood transfus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/80
Inventor 朱晓霞王惠民施秀英
Owner JIANGSU ZHONGSHENG MEDICAL DIAGNOSTIC REAGENT
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