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Nanoparticles, preparation method and application thereof and medicament

A nanoparticle and nanorod technology, applied in the field of nanoparticles, can solve the problems of low spatial resolution of photoacoustic imaging, high autofluorescence background, poor photothermal treatment effect, etc., achieve high spatial resolution, avoid abuse, improve The effect of photothermal therapy effect

Active Publication Date: 2019-04-05
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0005] The current photothermal antibacterial effect of gold nanomaterials is usually limited to photothermal therapy in the near-infrared region, but the photothermal therapy tissue penetration depth in the first region is shallow, the effect of photothermal therapy is poor, and the spatial resolution of photoacoustic imaging is relatively low. Low, but with high autofluorescence background

Method used

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  • Nanoparticles, preparation method and application thereof and medicament
  • Nanoparticles, preparation method and application thereof and medicament
  • Nanoparticles, preparation method and application thereof and medicament

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preparation example Construction

[0089] According to a second aspect of the present invention, there is provided a method for preparing the aforementioned nanoparticles, comprising the following steps:

[0090] Au / Ag nanorods are obtained by growing silver shells on the surface of gold nanorods.

[0091] The method for growing the silver shell layer can be carried out by using a conventional method for growing nanoparticles with a core-shell structure.

[0092] In one embodiment, the step of growing a silver shell layer on the surface of the gold nanorod comprises:

[0093] A surfactant solution, a silver source solution and a reducing agent solution are added to the gold nanorod-water dispersion liquid for mixed reaction to obtain Au / Ag nanorods.

[0094] The gold nanorod-water dispersion refers to a dispersion obtained by dispersing gold nanorods in water. The concentration of gold nanorods in the dispersion is, for example, 4 μg / mL, 5 μg / mL, 6 μg / mL, 7 μg / mL, 8 μg / mL, 9 μg / mL or 10 μg / mL.

[0095] A typ...

Embodiment approach

[0111] An exemplary embodiment, the preparation method of gold nanorods comprises the following steps:

[0112] (a) For every 0.1-20mL of 0.1-2mol / L gold source solution, add 0.1-20mL of 0.1-1mol / L surfactant solution and mix well; then, add 0.1-200μL of 0.1-2mol / L Mix the silver source solution evenly; add 0.1-2mL 0.1-2mol / L reducing agent solution after 60-3600min and mix evenly;

[0113] (b) Disperse the solution obtained in step (a) with a 1-1500W cell crushing device, add 0.1-200 μL of 0.1-2 mol / L fresh ice-cold reducing agent solution, and ultrasonically mix for 60 minutes after being treated by the cell crushing device for 1-10 minutes -3600min, to obtain gold nanorods.

[0114] It should be noted here that the above volumes are all relative amounts, for example, 0.1-20 mL of surfactant solution is added for every 0.1-20 mL of gold source solution.

[0115] The reducing agent in step (a) is ascorbic acid, and the reducing agent in step (b) is sodium borohydride.

[0...

Embodiment 1

[0166] A nanorod, the core is a gold nanorod with an aspect ratio of 4, and the shell is a 2nm thick silver shell.

[0167] A method for preparing nanorods, comprising the following steps:

[0168] (1) 0.17mL of 0.05M HAuCl 4 The solution was added into 20mL 0.1M CTAB solution, and stirred vigorously to mix the solution evenly, and the solution was bright brownish yellow. Then, 70 μL of 0.1M AgNO 3 Add dropwise to the above solution, and stir vigorously to mix the solution evenly. After 5 min, 0.12 mL of 0.1 M ascorbic acid solution was added, and the solution was mixed evenly with vigorous stirring, and the solution became colorless. Disperse the above solution with a 1500W cell disruptor, and immediately add 8 μL of 0.001M ice-cold NaBH 4 The aqueous solution was treated with a cell disruptor for 5 minutes and then stirred rapidly for 120 minutes. The solution turned purple, and the product Au NR was successfully prepared. Centrifuge the product at 13000rpm for 12min, d...

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Abstract

The invention discloses nanoparticles, a preparation method and an application thereof and a medicament, and relates to the technical field of nanoparticles. The nanoparticles comprise a gold nanorodinner core and a silver shell layer covering the outer surface of the gold nanorod, wherein the aspect ratio of the gold nanorod is 4-8, and Au / Ag / Au nanorods or Au / Ag / Au nanostars can be further prepared. The LSPR maximum absorption peak wavelength of the nanoparticles falls in the near infrared-II, photothermal conversion efficiency is higher than that of the near infrared-I, the photothermal treatment effect is good, and ablation of bacterial cells is further facilitated. Photoacoustic imaging has higher tissue penetration depth, long time and high spatial resolution. The antibacterial effect of Ag<+> released by an external Ag shell and photo-thermal sterilization effect of internal Au cooperate to achieve higher sterilization efficiency. The nanoparticles can be used for photoacousticimaging and bacterial infection diagnosis and treatment agents, and can non-invasively monitor local release and photothermal treatment processes of Ag<+>.

Description

technical field [0001] The invention relates to the technical field of nanoparticles, in particular, to a nanoparticle, its preparation method, application, and medicament. Background technique [0002] The discovery of antibiotics once saved millions of lives from deadly infections. However, with the long-term irregular use of antibiotics, drug-resistant bacteria have become a global problem; the most serious of which is methicillin-resistant grapevine. Cocci (Methicilin-resistant Staphylococcusaureus, MRSA). By the 1980s, MRSA had spread all over the world and became one of the common clinical pathogens. In recent years, the detection rate of MRSA has been on the rise, which can lead to diseases such as toxic shock syndrome and suppurative infection. Common antimicrobial drugs are ineffective, and the mortality rate is high. At present, MRSA has emerged multi-drug resistance, which poses an increasingly serious threat to human health and poses severe challenges to clinic...

Claims

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Application Information

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IPC IPC(8): B22F1/00B22F1/02C23C18/44A61K49/22A61K41/00A61K33/38A61K9/51A61P31/04
CPCA61K41/0052A61K49/225A61P31/04A61K9/5115A61K33/38C23C18/44B22F1/054B22F1/17A61K2300/00
Inventor 郑海荣胡德红盛宗海刘新
Owner SHENZHEN INST OF ADVANCED TECH
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