2results about How to "Avoid non-specific binding" patented technology

The invention discloses an amphiphilic polypeptide for liposome capture and transmembrane protein affinity activity analysis, and relates to the technical field of biology. The sequence of the amphiphilic polypeptide is GIGAVLKVLTTGLPALISWIKRLLNKLLKKLKLLKC, and the sequence of the amphiphilic polypeptide is GIGAVLKVLTTGLPALISWIKRLLNKLLKLLLKC. The amphiphilic polypeptide provided by the invention can be used for capturing and analyzing lipidosome, and compared with traditional hydrophobic alkyl capture, the amphiphilic polypeptide has stronger specificity and can effectively avoid non-specific binding. The lower non-specific binding enables the affinity analysis between the protein on the liposome and the ligand thereof to be possible.

The application discloses a kind of azahemicyanine dyes and application thereof, compared with the dye similar to the function of novel azahemicyanine dye molecule provided by the application, the application can effectively prevent the non-specific combination of serum albumin and hemicyanine dye, and the stability, fluorescence quantum yield and stoke shift of the hemicyanine dye are enhanced;The dye synthesis step provided by the application is simple, easy to purify, and has high yield;By introducing six-membered heterocyclic ring into the side chain of azahemicyanine dye, the combination with protein is effectively avoided, the false positive interference of serum albumin on dye fluorescence signal is excluded, and kidney targeting is realized, and the high fidelity of imaging is improved;The probe NA-H2S2 synthesized based on the dye NA-6 provided by the application is used for rapid detection of the change of H2S2 concentration;The synthesis step of the dye molecule is simple, easy to purify, has excellent imaging performance, fluorescence signal is not interfered by serum albumin, and is suitable for high-fidelity imaging of kidney.