50results about How to "Avoid non-specific binding" patented technology

The invention discloses an NT-ProBNP detection kit. The NT-ProBNP detection kit comprises a calibrator, a cleaning solution, a substrate solution, a pretreatment solution, an enzyme conjugate workingsolution and a magnetic bead conjugate working solution; the pretreatment solution contains pyridine, the enzyme conjugate working solution contains NT-ProBNP antibody labeled by enzyme, and the magnetic bead conjugate working solution contains magnetic beads labeled by the NT-ProBNP antibody. The NT-ProBNP detection kit can accurately measure NT-ProBNP in a whole blood sample, the lowest limit detection of the kit is 20 pg/ml, the linearity range is 20-5,000 pg/ml, the detection sensitivity is high, the linearity range is wide, and the result is accurate. The invention further discloses a using method of the NT-ProBNP detection kit. The NT-ProBNP detection kit is simple in using step, the detection time of an NT-ProBNP is shortened, and quick and sensitive detection of the NT-ProBNP is achieved.

The invention discloses FISH probe groups for detecting a novel coronavirus SARS-CoV-2. The probe groups comprise at least two fluorescent probe groups among a first fluorescent probe group taking anS gene as a target, a second fluorescent probe group taking an E gene as a target, a third fluorescent probe group taking an M gene as a target, a fourth fluorescent probe group taking an ORF 3a geneas a target, a fifth fluorescent probe group taking an N gene as a target, and a sixth fluorescent probe group taking an ORF 1ab gene as a target. In the at least two fluorescence probe groups, fluorescence molecules labeled by at least one fluorescence probe group have a fluorescence emission spectrum different from that of the other fluorescence probe groups. The FISH probe groups have high specificity and sensitivity, can realize positioning detection of SARS-CoV-2 in a to-be-detected sample, obtain the distribution and relative quantification conditions of the SARS-CoV-2, are an effectivesupplement for novel coronavirus nucleic acid detection, and have important clinical application value.

The invention belongs to the field of in-vitro diagnostic reagent kits, and particularly relates to a myoglobin detection reagent kit and a method for applying the same. The myoglobin detection reagent kit comprises calibration products, reagents R1, enzyme conjugate working solution R2, magnetic bead conjugate working solution M, cleaning solution and chemiluminescent substrates. The reagents R1contain imidazole components, blood cells in whole blood can be quickly removed by the imidazole components, and accordingly the possibility that magnetic beads are swallowed by the blood cells can beeffectively prevented; the cleaning solution contains sodium lauryl sulfate components, accordingly, cleaning effects can be enhanced, and non-specific binding of the chemiluminescent substrates canbe prevented. The myoglobin detection reagent kit and the method have the advantages that finger peripheral whole blood or anticoagulant venous whole blood can be directly used as a to-be-detected sample for the myoglobin detection reagent kit, the whole blood sample can be directly detected without being pretreated, accordingly, the detection speed can be greatly increased, operation steps can besimplified, the application range of the myoglobin detection reagent kit can be expanded, and large-scale popularization and application can be facilitated.

The invention discloses a gene detection product for genetic deafness, and particularly relates to a reagent kit for detecting multiple deafness genes at the same time. The reagent kit uses multiple PCR amplification primers and single base extension primers to simultaneously detect multiple hotspot mutations in the same tube, and has the advantages of quick diagnosis, easy operation and low cost. The invention also discloses a method for combining multiple PCR- single base extension-mass spectrum, and the method can be used to detect the genotype of the deafness gene accurately, sensitively and in high throughput, thereby being helpful for the popularization of deafness gene screening.

The invention provides a photoelectrochemical biosensor as well as a preparation method and application thereof, and belongs to the technical field of detection and analysis. The photoelectrochemicalbiosensor comprises: an electrode, which comprises an electrode substrate and a covalent organic polymer film coating the electrode substrate, wherein the covalent organic polymer film is modified with palladium nanoparticles and an aptamer; and the covalent organic polymer film is prepared by carrying out a polymerization reaction on 2,6-dihydroxynaphthalene-1,5-diformaldehyde and tris(4-aminophenyl)amine. The cathode photoelectrochemical cell sensor is successfully constructed by in-situ synthesis of the covalent organic polymer film, can be used for detecting various cells such as cancer cells by changing the type of the aptamer, and has high sensitivity and strong specificity, so that the cathode photoelectrochemical cell sensor has a good practical application value.

The invention discloses a method for detecting microRNA in lung cancer cells based on a non-substrate and non-labeled electrocatalytic amplification biosensor. The preparation method of the adopted biosensor comprises the following steps of: heating dicyandiamide and ferrous salt to 490-510 DEG C in an inert gas atmosphere to self-assemble to form a precursor, heating the precursor to 890-910 DEGC in the inert gas atmosphere, and performing calcining to obtain iron-containing nitrogen-rich carbon nano-tubes; and modifying the iron-containing nitrogen-rich carbon nano-tubes and gold nano-particles to a glassy carbon electrode to obtain an AuNP/ FeCN/ GCE electrode, and fixing a thiolated capture probe on the AuNP/ FeCN/ GCE electrode through a gold-sulfur bond, wherein the capture probe issingle-stranded DNA capable of hybridizing with target microRNA. In the invention, under the condition of no H2O2, a large amount of thiophen introduced by electrocatalytic reduction greatly enhanceselectrochemical signals.

The invention relates to a monkey pox virus antigen detection kit and a preparation method thereof, and belongs to the technical field of in vitro diagnostics, the monkey pox virus antigen detection kit comprises a detection card and a sample extracting solution, the detection card comprises an upper cover, a lower cover and a monkey pox virus antigen detection test strip, the monkey pox virus antigen detection test strip comprises a sample pad, a silicon core gold shell combination pad, a solid-phase antibody reaction membrane, absorbent paper and a PVC plate, a sample adding hole and an observation window are formed in the upper cover, and the preparation method of the monkey pox virus antigen detection kit comprises a preparation method of the silicon core gold shell combination pad, a preparation method of the sample pad and a preparation method of the solid-phase antibody reaction membrane. The kit can detect various samples including oropharynx swabs, body fluid, blood and skin focus tissues (including vesicular fluid, pustule fluid and scab), is high in sensitivity, and can effectively shorten the window period of virus diagnosis so as to reduce the hidden transmission risk caused by the incubation period and effectively control the large-scale transmission of epidemic situations.

The invention provides a novel 2019 coronavirus antigen detection reagent and a preparation method thereof. The detection reagent comprises a sample pad, a conjugate pad, an NC membrane and an absorption pad. The product is simple and convenient to operate, short in detection time, high in sensitivity and very suitable for large-scale preliminary screening of primary medical institutions and centralized outbreak areas of epidemic situations.

The invention relates to an antibody diluent for enhancing an immune imprinting detection signal. Each 1L of antibody diluent comprises the following components in percentage by weight: 0.25-2.5g of polyvinyl alcohol, 0.25-2.5g of polyvinylpyrrolidone, 0.1-2g of surfactant, 0.015-0.75mL of Kathon preservative and the balance of 0.01-0.1moL/L TBS buffer solution, wherein the pH of the TBS buffer solution is 7.2-7.6. The antibody diluent for enhancing the immune imprinting detection signal can be used for antibody dilution of immune imprinting detection, so that a relatively high background generated by pollution caused by protein is avoided; a relatively strong signal-to-noise ratio is displayed; and meanwhile, the strength and the sensitivity of the immune imprinting signal are enhanced.

The invention belongs to the field of oncology and medical examination, and relates to a kit for identifying circulating tumor cells by combining TCPP with a CEP probe and application. The kit comprises a TCPP stock solution, a subacid buffer solution, a cell cleaning solution, a cell fixing solution, a tissue fixing solution, a CEP probe solution, a cell membrane rupturing solution, a DAPI staining solution and PBS. The kit is applied to non-disease diagnosis and treatment purposes, and the method for identifying the circulating tumor cells by the kit comprises a solid-phase staining method or a liquid-phase staining method. The method does not depend on antigen expression on the surfaces of tumor cells as a detection means. False negative caused by antigen loss of tumor cells in the EMTprocess due to some existing antigen-dependent detection means is avoided, various epithelial tumors can be identified, and the defect that no unified tumor marker is provided for identifying circulating tumor cells due to heterogeneity of different types of tumor cells is avoided.

The invention discloses an antibody screening reagent for enhancing immunosuppression turbidimetry and a preparation and use method thereof. The method for enhancing immunosuppression turbidimetry overcomes the defects of the method for enhancing immune turbidimetry in the prior art in the principle of immune reaction. When no antigen exists in a measurement reaction system, the antigen connectedto latex particles generates agglutination reaction with the limited amount of antibody in the system, and the agglutination effect is maximized. When an antigen exists in the measurement reaction system, since the amount of antibody is limited, free antigen is excessive antigen which can inhibit the agglutination effect of the antigen-antibody complex, therefore, as the amount of free antigen inthe reaction system increases, the agglutination effect of the antigen-antibody complex is decreased. When the amount of antigen in the measurement system reaches a certain amount, the free antigen will completely inhibit the binding of the antibody and the antigen on the latex, and if the nonspecific binding of excess antigen and other substances in the latex and sample can be overcome, the turbidity change during the reaction will be zero.

The invention relates to a noninvasive female corpus luteum function monitoring technology, in particular to a full-quantitative or semi-quantitative rapid noninvasive urine test technology for determination of cyclic production amount of progesterone metabolites in women's urine so as to monitor and evaluate a female corpus luteum function, judge different types of ovulation cycle, determine whether ovulation is really performed or not and impregnation can be achieved or not after ovulation. According to the first implementation scheme, a protein hybridized couplet for determining pregnanediol glucuronide is related and contains pregnanediol glucuronide and a coupling protein. The protein hybridized couplet is characterized by further containing a junctional complex that connects the two parts. PdG of the hormone protein couplet is detected without an unsaturated labelled antibody, the sensitivity can be up to 0.1 ng/ml, and the linear range can be controlled to be 0.1-50 ng/ml.

The invention provides a novel coronavirus detection kit and a detection method thereof, and belongs to the technical field of molecular biological detection. A series of primer probe groups for detecting the novel coronavirus are redesigned, an existing detection method is improved, and detection targets are further increased, so that the detection sensitivity of the novel coronavirus is improved, false negative results can be remarkably reduced especially for detection of low-copy-number samples, the sensitivity and the accuracy of detection are effectively improved, and high-efficiency, high-specificity and low-cost detection is realized. The kit can be used for qualitatively detecting the novel coronavirus genes in pneumonia suspected cases and suspected aggregated case patients infected by the novel coronavirus in vitro and samples such as nasopharyngeal swabs and sputum of other patients needing novel coronavirus infection diagnosis or differential diagnosis.

The invention discloses a biotin and cell-penetrating peptide co-mediated breast cancer targeted intelligent liposome material. The intelligent liposome material is prepared by the following steps: 1,modifying biotin on a PEG long chain, and connecting the biotin with cholest through an acid-sensitive bond (a general formula I, a ligand material a); and 2, connecting cell-penetrating peptide R8 with phospholipid through Michael addition reaction (a formula II and a ligand material b). After the two ligand materials are combined to prepare a liposome, the biotin exposed on the surface of the liposome can specifically recognize an overexpressed SMVT transporter on the surface of a breast cancer cell; and after the liposome reaches the breast tumor part, a PEG long chain connected with a hydrazone bond is broken and separated, and the R8 connected with a short chain is mediated, passes through membrane and enters the cell, so that the effect of strongly treating the breast cancer is realized. The novel intelligent liposome material can be used for different dosage forms including liposome, nanoparticles, micelles and the like, and a prepared paclitaxel-loaded liposome has strong breast cancer penetrability and treatment effect and has a wide application prospect.

The invention provides a human SDC2 gene methylation detection kit, which comprises an enzyme digestion reaction solution and a fluorescent PCR (polymerase chain reaction) solution, wherein the enzymedigestion reaction solution and the fluorescent PCR reaction solution are packaged in a same PCR amplification tube in layered form through a hot melting material, and the enzyme digestion reaction solution occurs at the bottom of the PCR amplification tube. According to the kit disclosed by the invention, methylation sensitive restriction endonuclease and marking twin primers are utilized to carry out enzyme digestion reaction and fluorescent PCR sequentially in one tube so as to detect the methylation state of the SDC2 gene; the kit has the advantages of high operation speed, good operationsimplicity, high sensitivity, good specificity and good convenience of automation.

The present invention relates to the technical field of vascular endothelial growth factor detection, and discloses a raw material preparation method and a detection method of a vascular endothelial growth factor detection kit. Theraw material preparation method comprises raw material preparation, the raw material preparation comprises an antibody coated magnetic bead working solution, a VEGF labeled antibody, a VEGF calibration product, a VEGF quality control product and a sample diluent; the antibody coated magnetic bead working solution is a magnetic microsphere suspension coated with a Fab segment of a humanized VEGF antibody, and the buffer system is TBS-T (20-200 mM Tris, 0-1 M NaCl, 0.0%-1% BSA, 0.1%-0.5% Tween-20 and 0.1%-1% Proclin-300, and the pH value is 6.5-9.0). According to the present invention, the selected luminous marker is acridine salt NSP-SA-NHS; therefore, luminescence detection is carried out in homogeneous liquid, and the reaction time is shortened. Meanwhile, the bond level of C-N is larger than that of C-O, and the stability of the conjugate of the acridine salt NSP-SA-NHS and the protein is higher. Based on the use of NSP-SA-NHS, the sensitivity, the detection range and the storage time of the detection system are improved, and the reaction time is shortened.