Biomarker for diagnosing mycobacterium tuberculosis infection and related kit

A biomarker, Mycobacterium tuberculosis technology, applied in the field of biological diagnosis, can solve the problems of inability to determine the results, insufficient sensitivity, etc., to achieve the effect of improving the diagnostic efficiency, rapid operation, high sensitivity and specificity

Inactive Publication Date: 2017-03-15
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, if IFN-γ is the only diagnostic marker, its sensitivity is still insufficient. In addition, in patients with HIV combined with tuberculosis or other tuberculosis patients with immunodeficiency, the amount of IFN-γ released after positive control...

Method used

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  • Biomarker for diagnosing mycobacterium tuberculosis infection and related kit
  • Biomarker for diagnosing mycobacterium tuberculosis infection and related kit
  • Biomarker for diagnosing mycobacterium tuberculosis infection and related kit

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Example 1: Screening of biomarkers for diagnosis of Mycobacterium tuberculosis infection

[0032] Step 1: Case collection

[0033] Subject samples were collected, including blood samples from patients with active tuberculosis, patients with latent tuberculosis infection and healthy controls. The basis for the diagnosis of active pulmonary tuberculosis in the patient is: the patient has a positive sputum smear result or a positive sputum culture result, abnormal X-ray chest film or less than 30 days of anti-tuberculosis treatment; the inclusion criteria for patients with latent tuberculosis infection are: High-risk factors for tuberculosis infection or close contact with active tuberculosis patients, tuberculosis-specific or The test was positive, and there was no evidence of tuberculosis infection in chest radiographs and sputum smears; healthy controls had no high-risk factors for tuberculosis infection, no recent history of close contact with active tuberculosis, ...

Embodiment 2

[0059] Example 2: Clinical application of biomarkers for diagnosis of Mycobacterium tuberculosis infection and related kits

[0060] Step 1: Case collection

[0061] Suspected patients with active tuberculosis were collected clinically, and the inclusion criteria were: clinical manifestations similar to active tuberculosis, such as cough, hemoptysis, weight loss, fever, and night sweats, etc. High-risk factors for infection or close contact history of active tuberculosis patients. In this example, according to the above inclusion criteria, a total of 44 suspected active tuberculosis patients were collected.

[0062] Step 2: Specimen Collection and Processing

[0063] Take 5-10ml of venous blood from the collected peripheral blood of suspected patients with active tuberculosis into vacuum blood collection tubes; further separate peripheral blood mononuclear cells: centrifuge the vacuum blood collection tubes at a speed of 2800-3000rpm, and the centrifugation time After the c...

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Abstract

The invention discloses a biomarker for diagnosing mycobacterium tuberculosis infection. The biomarker comprises IL-2 and IL-10. The invention proves that cell factors IL-2 and IL-10 can serve as markers for tuberculosis infection diagnosis for the first time, the tuberculosis infection can be diagnosed by measuring the concentration of the IL-2 and the IL-10 after peripheral blood monouclear cells are stimulated by tuberculosis antigen, and active tuberculosis and latent tuberculosis infection are further distinguished. A tuberculosis infection blood detection reagent and a kit which are prepared on the basis of the two cell factors provide new technological basis for quick diagnosis of the active tuberculosis, have the advantages of high sensitivity, high specificity and the like, are quick in operation, reasonable and practical, and can obviously improve the diagnosis efficiency of the active tuberculosis.

Description

technical field [0001] The invention relates to the technical field of biological diagnosis, in particular to a biomarker for diagnosing Mycobacterium tuberculosis infection and a related kit. Background technique [0002] The current immunodiagnostic methods for tuberculosis still cannot meet the needs of rapid and early diagnosis of tuberculosis and screening of latent tuberculosis infection. Since the cell-mediated immune response plays a key role in the human body's fight against Mycobacterium tuberculosis infection, finding Mycobacterium tuberculosis-specific T cell antigens and detecting T cell immune responses have become research hotspots in recent years. [0003] The development and application of IFN-γrelease assays (IFN-γrelease assays, IGRAs) has brought new progress to the diagnosis of tuberculosis, and these two tests have also been applied to a certain extent in the clinical detection of tuberculosis. IGRAs application tester's peripheral whole blood or isola...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/569
CPCG01N33/6869G01N33/5695G01N2333/5428G01N2333/55
Inventor 王森吴晶张文宏张颖
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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