Joint prosthesis infection diagnosis kit

A diagnostic kit and joint prosthesis technology, applied in DNA/RNA fragmentation, microbial determination/examination, recombinant DNA technology, etc., can solve the problem of insufficient specificity, achieve high sensitivity and specificity, and improve detection accuracy , the effect of high detection accuracy

Active Publication Date: 2018-05-15
NINGBO MEDICAL CENT LIHUILI HOSPITACL
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Problems solved by technology

Prokaryotic cell-specific ribosomal gene (16S rRNA) is different from eukaryotic cells and is shared by all prokaryotic cells. In the chromosomes of organisms, it is used as an indicat...

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  • Joint prosthesis infection diagnosis kit
  • Joint prosthesis infection diagnosis kit

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Embodiment

[0057] A diagnostic kit for joint prosthesis infection, comprising primers, magnesium ion-free PCR reaction buffer, MgCl 2 , Taq polymerase, dNTP mix and ddH 2 O, the primers include universal primers, specific primers and internal reference primers, and the specific primers are composed as follows:

[0058] ica D gene detection primers: upstream primer 5'-ATCGTTGTGATGATTGTTTA-3' (SEQ ID No.3) and downstream primer 5'-GATATGTCACGACCTTTCTT-3' (SEQ ID No.4);

[0059] eno gene detection primers: upstream primer 5'-ACGTGCAGCAGCTGACT-3' (SEQ ID No.5) and downstream primer 5'-CAACAGCATYCTTCAGTACCTTC-3' (SEQ ID No.6);

[0060] sar A gene detection primers: upstream primer 5'-CATCAGCGAAAAGAGAAA-3' (SEQ ID No.7) and downstream primer 5'-TGTTTGCTTCAGTAATTCGTTT-3' (SEQ ID No.8);

[0061] agr gene detection primers: upstream primer 5'-GCGCCATAGGATTGTAGAGTG-3' (SEQ ID No.9) and downstream primer 5'-CTCAGTAAGAATCCATTTCGCCC-3' (SEQ ID No.10);

[0062] Mec A gene detection primers: upstrea...

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Abstract

The invention discloses a joint prosthesis infection diagnosis kit. The joint prosthesis infection diagnosis kit comprises primers, a magnesium-ion-free PCR reaction buffer, MgCl2, Taq polymerase, a dNTP mixture and ddH2O, wherein the primers comprise a universal primer, a specificity primer and an internal reference primer; the specificity primer consists of the following compositions: an ica D gene detection primer, an eno gene detection primer, a sar A gene detection primer, an agr gene detection primer, a Mec A gene detection primer and a Mre B gene detection primer. The joint prosthesis infection diagnosis kit is high in joint prosthesis infection diagnosis, particularly early diagnosis, the detection accuracy is high, infection can be detected rapidly and accurately in the early period when the joint prosthesis infection does not have clinical symptoms, therapeutic measures can be taken in time, and further deterioration of the state of an illness is prevented.

Description

technical field [0001] The invention relates to the technical field of diagnosis of joint prosthesis infection, in particular to a diagnostic kit for joint prosthesis infection. Background technique [0002] Prosthetic joint infections (PJI) are recognized as one of the most serious complications after joint replacement surgery. Although the incidence of PJI is not high, it has not been significantly reduced since the application of antibiotics. According to reports, the incidence of hip and shoulder PJI is about 1%, the incidence of knee PJI is about 2%, and the incidence of elbow PJI is about 9%; and for arthroplasty, the incidence of PJI is even as high as 40%; Additionally, PJI was responsible for 33% of the complications leading to failure of total ankle replacement surgery. [0003] Today, in the face of PJI, the most troublesome problem for clinicians is the difficulty of early diagnosis. When clinical symptoms of infection appear, it is mostly late, which often dela...

Claims

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Application Information

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IPC IPC(8): C12Q1/689C12N15/11C12Q1/04
CPCC12Q1/689
Inventor 彭兆祥李瑾郑慷林杰王思佳
Owner NINGBO MEDICAL CENT LIHUILI HOSPITACL
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