Immune colloidal metal detection technology capable of directly performing semi-quantitative analysis, preparation method and application

A detection technology, colloidal metal technology, applied in the field of immune colloidal metal detection technology, can solve the problems of dynamic changes that cannot determine the value of clinical diagnosis and treatment, etc., and achieve the effects of shortening the detection time, convenient and quick use, and simple operation

Active Publication Date: 2011-07-27
CHANGZHOU BIOWIN BIOPHARM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, if the currently widely used detection technology based on solid-phase carrier reaction is not equipped with corresponding detection equipment and only relies on the naked eye to judge the results, it is still limited to a qualitative level, and can only be judged at a certain detection level. For example, the minimum detectable amount of human chorionic gonadotropin in urine of early pregnancy colloidal gold method detection test paper used for early pregnancy detection is 25U/L, while the concentration of human chorionic gonadotropin in nor

Method used

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  • Immune colloidal metal detection technology capable of directly performing semi-quantitative analysis, preparation method and application
  • Immune colloidal metal detection technology capable of directly performing semi-quantitative analysis, preparation method and application
  • Immune colloidal metal detection technology capable of directly performing semi-quantitative analysis, preparation method and application

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Embodiment 1

[0040] Embodiment 1-making of the chromatography detection kit of the present invention

[0041] Such as figure 1 As shown, the basic structure of the chromatography method of the present invention consists of a solid-phase membrane 1, a support sheet 2, a chromogenic agent 3, a spotting device 4, a sample loading buffer 5, a standard reference product 6, a spotting window 7, a mixing Sample tank 8, diluent 9, dropper 10, sample pad 11 and water-absorbing pad 12, wherein sample pad 11, membrane pad sprayed with color developer 3, solid-phase membrane 1 and water-absorbing pad 12 are attached to support sheet 2 in sequence Above, the standard reference product 6 with different concentrations is pre-transferred and immobilized on the solid phase membrane 1 . The sample to be tested is transferred to the sample mixing tank 8 with the dropper 10, and an appropriate amount of diluent 9 is dropped into the sample mixing tank 8 as required, mixed with the sample, and a certain amoun...

Embodiment 2

[0042] Embodiment 2-The making and experimental observation of C-reactive protein colloidal gold chromatography semi-quantitative detection kit of the present invention

[0043] Experimental materials: C-reactive protein, anti-human C-reactive protein monoclonal antibody, chloroauric acid, trisodium citrate, potassium carbonate, crystalline bovine serum albumin, nitrocellulose membrane, PVC film, polyester film, absorbent paper , sample pad, micro-injector, dropper, bovine serum sample buffer, normal human serum samples and serum samples from patients with acute myocardial infarction.

[0044] Method: Use deionized water to make chloroauric acid into a solution with a concentration of 0.01%, heat it to boiling, add 100ul of 10% trisodium citrate solution per 100ml, stir quickly and evenly, and wait until the color becomes wine red and stable , continue heating for 10 min, cool to room temperature, put into reagent bottles, and save for later use. Take 10ml of the prepared col...

Embodiment 3

[0051] Embodiment 3-making and experimental observation of human myoglobin colloidal gold chromatography semi-quantitative detection kit of the present invention

[0052] Experimental materials: human myoglobin, anti-human myoglobin monoclonal antibody, chloroauric acid, trisodium citrate, potassium carbonate, crystalline bovine serum albumin, nitrocellulose membrane, PVC film, polyester membrane, absorbent paper, Sample pads, microsamplers, droppers, bovine serum loading buffer, normal human serum samples and acute myocardial infarction patient serum.

[0053] Method: Use deionized water to make chloroauric acid into a solution with a concentration of 0.01%, heat it to boiling, add 100ul of 10% trisodium citrate solution per 100ml, stir quickly and evenly, and wait until the color becomes wine red and stable , continue heating for 10 min, cool to room temperature, put into reagent bottles, and save for later use. Take 10ml of the prepared colloidal gold solution, adjust the ...

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Abstract

The invention discloses a semi-quantitative solid-phase reaction detection technology for content of an object to be detected, a preparation method and application. The technology is a semi-quantitative colloidal metal immune chromatographic detection technology capable of directly detecting the content of the object to be detected, in which the sample of the object to be detected is reacted with a standard comparison product of the object to be detected with known concentration under the same reaction system, wherein a solid-phase membrane, a supporting sheet, a developing agent, a sample applicator and the standard comparison product are provided in the technology; various ready-to-use products for detecting the content of the object to be detected can be prepared; and the technology can be effectively used for clinical detection and detection on the content of the object to be detected, such as foods, medicaments, laboratory supplies and the like, and has high application value and market prospect.

Description

[0001] [Technical field] An immune colloidal metal detection technology, especially a colloidal metal immunochromatographic detection technology capable of direct semi-quantitative analysis and its preparation method and application. [Background technique] [0002] Colloidal metal labeling technology is a new type of immunolabeling technology that uses colloidal metal as a tracer marker or chromogenic agent and is applied to antigen-antibody reactions. Because it does not have problems such as endogenous enzyme interference and radioactive isotope contamination, and colloidal metals of different particle sizes can be used for double or even multiple labeling, making the positioning more accurate. Therefore, it has become a new type of labeling technology after luciferin, enzyme, isotope and latex labeling technology. It has been widely used in electron microscopy, flow cytometry, western blotting, in vitro diagnostic reagents, and the manufacture of food and drug quality testi...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/545G01N33/532
Inventor 刘凤鸣
Owner CHANGZHOU BIOWIN BIOPHARM
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