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Nano-colloidal gold marker immunization measurement method for testing carbofuran pesticide

A nano-colloid and immunoassay technology, which is applied in the direction of measuring devices, biological testing, material inspection products, etc., to achieve the effects of stable sensitivity, simple reaction and operation, and fast detection speed

Inactive Publication Date: 2003-10-01
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to overcome the deficiencies in the prior art, to provide a nano-colloidal gold-labeled immunoassay method for carbofuran pesticides, so that nanotechnology and immune technology can be applied to the field of pesticide residue detection, changing the traditional multi-step method for pesticide residues One-step rapid detection of carbofuran pesticide residues due to cumbersome detection problems

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The immune hapten was synthesized by thermal method. Take 100g of furanol and add it to a 250ml three-necked flask, then add 19g of catalyst trimethylbenzyl ammonium chloride, heat to 95°C with electric stirring, and pass phosgene into it at a certain flow rate for 2 hours. Precipitation occurred, and structural characterization was carried out by sampling, gas spectrometry, and mass spectrometry. Then weigh 4.6g of 6-aminocaproic acid and dissolve it in 6.4ml of 4mol / L NaOH solution, add it to a 250ml flask, cool to 4°C in an ice bath, weigh 7.18g of benzofuryl chloroformate and dissolve it in 20ml of dioxane In the three-neck flask, under the condition of stirring, it was added into a three-necked flask five times at intervals of 10 minutes and reacted for two hours. The pH value was adjusted to 4.0, extracted three times with ethyl acetate, and a white precipitate was precipitated, washed with water and dried.

[0022] Then, the immune antigen and the coating antigen...

Embodiment 2

[0026]The immune hapten was synthesized by thermal method. Take 100g of furanol and add it to a 250ml three-necked flask, then add 19g of catalyst trimethylbenzyl ammonium chloride, heat to 95°C with electric stirring, and pass phosgene into it at a certain flow rate for 2 hours. Precipitation occurred, and structural characterization was carried out by sampling, gas spectrometry, and mass spectrometry. Then weigh 4.6g of 6-aminocaproic acid and dissolve it in 6.4ml of 4mol / L NaOH solution, add it to a 250ml flask, cool to 4°C in an ice bath, weigh 7.18g of benzofuryl chloroformate and dissolve it in 20ml of dioxane In the three-neck flask, under the condition of stirring, it was added into a three-necked flask five times at intervals of 10 minutes and reacted for two hours. The pH value was adjusted to 4.0, extracted three times with ethyl acetate, and a white precipitate was precipitated, washed with water and dried.

[0027] Then, the immune antigen and the coating antigen ...

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Abstract

A nano colloidal golf linked immunoassary for detecting carbofuran includes such steps as coupling the immunized semi-antigen of carbofuran with BSA, synthesizing artificial immunoantigen compound, immunizing animal to prepare specific high-valence BFNH antibody, separating and purifying, linking the nano-class colloidal golf to said specific antibody, solidifying said linked compound, carbofurancoupled by egg albumin and sheep's mouse IgG antibody on a carrier, and for semi-quantitative analysis. Its advantage is high sensitivity and speed.

Description

technical field [0001] The invention relates to an application of immunochemical detection technology to the detection of pesticide residues, in particular to a nano colloidal gold-labeled immunoassay method for carbofuran pesticide. The invention belongs to the field of pesticide residue detection. Background technique [0002] Carbofuran (2,3-dihydro-2,2-dimethyl-7-phenylpropanylmethylcarbamate), also known as carbofuran, is a broad-spectrum, high-efficiency, long-lasting drug effect Systemic insecticide, acaricide, and nematicide are widely used in the pest control of rice, corn, tobacco, tea, soybean, gum white, leek and other crops. Since the 1970s, the toxicological research on carbofuran has found that carbofuran forms a complex with cholinesterase in the body, inhibits its activity, prevents acetylcholine from being decomposed and accumulates in the body, and affects the impulse between central nervous cells Conduction, neurotoxic. The drug remains in the soil for...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/532G01N33/558
Inventor 周培陆贻通李斌周军
Owner SHANGHAI JIAO TONG UNIV
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