Test paper based on photosensitive meso-porous silicon-based molecular imprinting microsphere and preparation method thereof

A technology of molecularly imprinted microspheres and test strips, which is applied in the direction of chemical reaction of materials for analysis, and material analysis by observing the influence of chemical indicators, to enhance the adsorption capacity, solve the problem of adsorption residues, and be widely used. Effect

Inactive Publication Date: 2017-07-07
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Qualitative or semi-quantitative test paper detection is an intuitive and visual detection method. Therefore, combining molecular imprinting with test

Method used

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  • Test paper based on photosensitive meso-porous silicon-based molecular imprinting microsphere and preparation method thereof
  • Test paper based on photosensitive meso-porous silicon-based molecular imprinting microsphere and preparation method thereof
  • Test paper based on photosensitive meso-porous silicon-based molecular imprinting microsphere and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Preparation of detection test paper based on photosensitive mesoporous silicon-based molecularly imprinted microspheres

[0039] The test strip can change the template molecule according to the detection index. Take aflatoxin, anisidine, and sodium dodecylbenzenesulfonate (DBS), important markers of waste oil, as examples:

[0040] (1) Preparation of photosensitive mesoporous silicon-based molecularly imprinted microspheres:

[0041] a) Mesoporous SiO 2 Double-bond modification of nanospheres: using a sol-prepared method to mesoporous SiO 2 Nanospheres are used as the carrier, and 3-(trimethoxysilyl)-propyl methacrylate (MPS) is used as the raw material to introduce double bonds. Specifically, mesoporous SiO 2 Nanospheres were suspended in MPS solution, stirred magnetically at 50°C for 5 hours, collected and separated, washed repeatedly with water, and dried under vacuum at 50°C;

[0042] b) Preparation of photosensitive mesoporous silicon-based molecularl...

Embodiment 2

[0046] Example 2: Control of shell thickness of photosensitive mesoporous silicon-based molecularly imprinted microspheres and evaluation of core-shell binding firmness

[0047] Controlled Modification of SiO During Microsphere Surface Polymerization 2 , the molar ratio of the functional monomer and the initiator, the polymerization reaction temperature, the reaction time, etc. to obtain microspheres under different conditions, and the thickness of the microspheres was detected by TEM. Then take 50 mg of microspheres, place them in 5 mL centrifuge tubes, add 3.0 mL of methanol solutions with different concentrations of templates, shake on the shaker for 24 hours, take the supernatant and dilute with methanol, and then use an ultraviolet spectrophotometer to detect the concentration of template molecules. Concentrations were measured 3 times in parallel and the average value was taken. Then calculate the adsorption capacity of the microspheres. The conditions corresponding to...

Embodiment 3

[0062] Embodiment 3: minimum detection limit and selectivity evaluation of test strips

[0063] 1. Determination of the minimum detection limit of test strips

[0064] Aflatoxin, anisidine and DBS standard substances were made into solutions with serial concentrations (0-100 μg / mL), and 100 μL were added dropwise to the test strip sample pad prepared in Example 1 for detection, repeated 3 times ; By visual observation, the minimum concentration when the degree of discoloration of the test strip detection line is significantly less than the degree of change of the color of the control quality control line is the detection limit of the test strip by naked eyes; wherein the detection limit of aflatoxin is 0.5 μg / mL, the detection limit of anisidine was 0.5 μg / mL, and the detection limit of DBS was 1.0 μg / mL (Table 5).

[0065] Table 5 Molecularly imprinted microsphere detection test paper detection results of aflatoxin, anisidine and DBS

[0066]

[0067]

[0068] Note: +...

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Abstract

The invention discloses a test paper based on a photosensitive meso-porous silicon-based molecular imprinting microsphere and a preparation method thereof. The method comprises the following steps: by taking a mesoporous SiO2 nanosphere with the surface modified by double bond as a carrier, a to-be-detected object as a template molecule, azobenzene compound as a functional monomer, dimethyl acrylate as a cross-linking agent, azo isobutyronitrile as an initiator and acetonitrile as a pore-foaming agent, preparing the photosensitive meso-porous silicon-based molecular imprinting microsphere, and then removing the template molecule with a solvent under a light-operated effect, thereby acquiring the molecular imprinting microsphere. According to the technology, the thickness of the polymer shell of the nuclear shell type molecular imprinting microsphere can be strictly controlled, the regulation optimization of the adsorption capacity can be realized, the binding strength of the polymer shell and the mesoporous silicon core can be enhanced and the mechanical damage of the subsequent process to the molecular imprinting microsphere can be avoided. The test paper prepared according to the invention utilizes the photosensitive isomerization and photochromic principle of the functional monomer of the polymer to realize the photosensitive recognition of the molecular imprinting microsphere for the detected object and semi-quantitative analysis can be performed according to the color depth.

Description

technical field [0001] The invention relates to a detection test paper, in particular to a visual detection test paper based on photosensitive mesoporous silicon-based molecularly imprinted microspheres and a preparation method thereof. Background technique [0002] Molecularly imprinted polymers (MIPs) can selectively recognize imprinted trace / trace template molecules, and have been widely used in the pretreatment of complex sample analysis. In recent years, molecular imprinting technology has become a research hotspot at home and abroad, and the combination of molecular imprinting technology and solid phase microextraction technology is a new breakthrough in the field of separation and enrichment. [0003] The preparation of molecularly imprinted polymers mainly includes the following three steps: first, the template molecules and functional monomers are combined to form reversible compounds or complexes through covalent bonds or non-covalent bonds; Under the action of ag...

Claims

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Application Information

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IPC IPC(8): G01N21/78C08F292/00C08F220/34C08J9/26
CPCG01N21/78C08F292/00C08J9/26C08J2201/0424C08J2351/10C08F220/34
Inventor 谢练武邓慧芸王斌郭亚平吴茂
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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