Microcystic toxin ratio fluorescence sensor constructed based on molecular imprinting technology

A molecular imprinting technology, a technology of microcystins, applied in the fields of nanotechnology, nanotechnology, nanotechnology for materials and surface science, can solve the problem of low sensitivity of high performance liquid chromatography and can not meet the rapid monitoring of algal blooms. Timely analysis and other problems, to achieve the effect of good fluorescence response, meet the needs of rapid detection, and low cost

Pending Publication Date: 2021-11-16
LINYI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

High-performance liquid chromatography has low sensitivity and requires high-magnification enrichment, so it takes

Method used

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  • Microcystic toxin ratio fluorescence sensor constructed based on molecular imprinting technology
  • Microcystic toxin ratio fluorescence sensor constructed based on molecular imprinting technology
  • Microcystic toxin ratio fluorescence sensor constructed based on molecular imprinting technology

Examples

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Effect test

Embodiment 1

[0033] A microcystin ratiometric fluorescent sensor based on molecular imprinting technology, the preparation method is as follows:

[0034] The first step: Synthesis of fluorescent carbon dots CQDS: Dissolve 0.3g of anhydrous citric acid in 10mL of N-(β-aminoethyl)-γ-aminopropyl-methyldimethoxysilane, and fill 50mL of Teflon Long-lined stainless steel autoclave, and degassed with nitrogen for 20 minutes; then, the autoclave was kept at 240 ° C for 2 hours, cooled to room temperature; the solution was filtered with a filter membrane; then washed three times with petroleum ether, and the obtained The product was dispersed in absolute ethanol to obtain a fluorescent carbon dot CQDS ethanol solution, and stored in a refrigerator at 4°C for further use;

[0035] Step 2: FITC-APTES-SiO 2 Preparation of composite nanoparticles: Mix 4mg FITC with 10mL water, stir evenly with magnetic force at room temperature, add 100μL 3-aminopropyltriethoxysilane APTES, and stir at constant temper...

Embodiment 2

[0039] A microcystin ratiometric fluorescent sensor based on molecular imprinting technology, the preparation method is as follows:

[0040] Step 1: Synthesis of fluorescent carbon dots CQDS: Dissolve 0.6g of anhydrous citric acid in 10mL of N-(β-aminoethyl)-γ-aminopropyl-methyldimethoxysilane, and fill in 50mL of Teflon Long-lined stainless steel autoclave, and degassed with nitrogen for 20 minutes; then, the autoclave was kept at 240 ° C for 2 hours, cooled to room temperature; the solution was filtered with a filter membrane; then washed three times with petroleum ether, and the obtained The product was dispersed in absolute ethanol to obtain a fluorescent carbon dot CQDS ethanol solution, and stored in a refrigerator at 4°C for further use;

[0041] Step 2: FITC-APTES-SiO 2 Preparation of composite nanoparticles: Mix 4-10 mg of FITC with 10 mL of water, stir evenly with magnetic force at room temperature, add 100 μL of 3-aminopropyltriethoxysilane APTES, and stir at const...

Embodiment 3

[0045] A microcystin ratiometric fluorescent sensor based on molecular imprinting technology, the preparation method is as follows:

[0046] The first step: Synthesis of fluorescent carbon dots CQDS: Dissolve 0.5g of anhydrous citric acid in 10mL of N-(β-aminoethyl)-γ-aminopropyl-methyldimethoxysilane, and fill 50mL of Teflon Long-lined stainless steel autoclave, and degassed with nitrogen for 20 minutes; then, the autoclave was kept at 240 ° C for 2 hours, cooled to room temperature; the solution was filtered with a filter membrane; then washed three times with petroleum ether, and the obtained The product was dispersed in absolute ethanol to obtain a fluorescent carbon dot CQDS ethanol solution, and stored in a refrigerator at 4°C for further use;

[0047] The transmission electron microscope picture of the obtained fluorescent carbon dot CQDS is as follows figure 1 shown.

[0048] The synthesis products of carbon dots include the decomposition and pyrolysis of anhydrous c...

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Abstract

The invention discloses a microcystic toxin ratiometric fluorescence sensor constructed based on a molecular imprinting technology, and belongs to the technical field of environment detection. According to the invention, carbon quantum dots taking citric acid as a carbon source and a nanomaterial of silicon dioxide-loaded fluorescein isothiocyanate are synthesized, the carbon quantum dots and the nanomaterial respectively have the characteristics of fluorescence quenching and fluorescence enhancement on microcystic toxins, so that a ratiometric fluorescence method is established, and then a molecular imprinting method is adopted, and metformin is taken as a false template, and the microcystic toxins are selectively recognized by leaving imprinting sites on the composite nano material, so that the effect of quantitative detection is achieved. The obtained CQDS-FITC-APTES-SiO2@MIP is good in microcystic toxin RR/LR fluorescence correspondence degree and sensitive in reaction, the fluorescence ratio technology and the molecular imprinting technology are combined, the rapid detection requirement of microcystic toxins is completely met, and the CQDS-FITC-APTES-SiO2@MIP is low in cost and suitable for being used in various occasions.

Description

technical field [0001] The invention belongs to the technical field of environmental detection, and in particular relates to a microcystin ratio fluorescence sensor constructed based on molecular imprinting technology. Background technique [0002] Microcystin (MC for short) is a class of natural toxins produced by cyanobacteria. It is a class of cyclic heptapeptide peptide hepatotoxins. Health poses a huge threat. In particular, MC-LR and MC-RR are attracting widespread attention as the most toxic and widespread microcystins. Microcystis sometimes exhibits extraordinary vitality. Regardless of conventional tap water treatment processes or boiling water, it is difficult to effectively remove microcystins. Studies have shown that microcystins can still retain a part of their activity even at a high temperature of 300 degrees Celsius. Therefore, sensitive and selective detection of microcystins is of paramount importance in the context of biological and environmental system...

Claims

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Application Information

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IPC IPC(8): G01N21/64C09K11/06C09K11/65B82Y30/00B82Y40/00
CPCG01N21/6428C09K11/06C09K11/65B82Y30/00B82Y40/00G01N2021/6417C09K2211/1088
Inventor 胡雪萍李苹宋兴良
Owner LINYI UNIVERSITY
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