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Kit, use method thereof, and hydrogen peroxide concentration detection method

A kit and concentration technology, applied in the direction of analysis, measurement device, color/spectral property measurement by chemical reaction of materials, can solve problems such as unfavorable multiple detections, and achieve simple detection methods, accurate results, and huge applications. effect of value

Pending Publication Date: 2022-02-01
HUBEI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, collecting blood lactic acid to measure the concentration is an invasive and invasive test, which is not conducive to multiple tests.

Method used

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  • Kit, use method thereof, and hydrogen peroxide concentration detection method
  • Kit, use method thereof, and hydrogen peroxide concentration detection method
  • Kit, use method thereof, and hydrogen peroxide concentration detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] The embodiment of the present application provides a method for using the kit, comprising the following steps:

[0079] S11, reacting 1mM chloroauric acid solution and 38.8mM sodium citrate solution at 100°C for 15min to obtain a gold nano solution;

[0080] S12. Dissolving 52 mg of 4-(2-hydroxyethyl)-1-piperazineethanesulfonate sodium (HEPES) in 100 mL of ultrapure water, and adjusting the pH to 7 with hydrochloric acid to obtain a HEPES buffer;

[0081] S13, dissolving cysteine ​​in HEPES buffer to prepare a 30 μM cysteine ​​solution;

[0082] S14, dissolving ferrous sulfate in HEPES buffer to prepare a 20 μM ferrous sulfate solution;

[0083] S15. Add 20 μL of 40 μM H 2 o 2 After mixing the sample solution with 20 μL of the cysteine ​​solution prepared in step S13, add 5 μL of the ferrous sulfate solution prepared in step S14 and mix for 4 minutes to obtain a mixed solution;

[0084] S16. Mix the mixed solution in step S15 with 45 μL of the gold nano solution obt...

Embodiment 2

[0106] The embodiment of the present application provides a method for using the kit, comprising the following steps:

[0107] S11, reacting 1mM chloroauric acid solution and 38.8mM sodium citrate solution at 100°C for 15min to obtain a gold nano solution;

[0108] S12. Dissolving 52 mg of 4-(2-hydroxyethyl)-1-piperazineethanesulfonate sodium (HEPES) in 100 mL of ultrapure water, and adjusting the pH to 7 with hydrochloric acid to obtain a HEPES buffer;

[0109] S13, dissolving cysteine ​​in HEPES buffer to prepare a 30 μM cysteine ​​solution;

[0110] S14, dissolving ferrous chloride in HEPES buffer to prepare a 20 μM ferrous chloride solution;

[0111] S15. Add 20 μL of 45 μM H 2 o 2 After mixing the sample solution with 20 μL of the cysteine ​​solution prepared in step S13, add 5 μL of the ferrous chloride solution prepared in step S14 and mix for 5 minutes to obtain a mixed solution;

[0112] S16. Mix the mixed solution in step S15 with 45 μL of the gold nano solution ...

Embodiment 3

[0120] The embodiment of the present application provides a method for using the kit, comprising the following steps:

[0121] S11, reacting 1mM chloroauric acid solution and 38.8mM sodium citrate solution at 100°C for 15min to obtain a gold nano solution;

[0122] S12. Dissolving 52 mg of 4-(2-hydroxyethyl)-1-piperazineethanesulfonate sodium (HEPES) in 100 mL of ultrapure water, and adjusting the pH to 7 with hydrochloric acid to obtain a HEPES buffer;

[0123] S13, dissolving cysteine ​​in HEPES buffer to prepare a 30 μM cysteine ​​solution;

[0124] S14, dissolving ferrous nitrate in HEPES buffer solution to prepare a 25 μM ferrous nitrate solution;

[0125] S15. Add 20 μL of 50 μM H 2 o 2 After mixing the sample solution with 20 μL of the cysteine ​​solution prepared in step S13, add 5 μL of the ferrous nitrate solution prepared in step S14 and mix for 5 minutes to obtain a mixed solution;

[0126] S16. Mix the mixed solution in step S15 with 45 μL of the gold nano sol...

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Abstract

The invention provides a kit, a use method thereof, and a hydrogen peroxide concentration detection method. The kit comprises a gold nano solution, a cysteine solution and a ferrous ion solution. The kit comprises ferrous ions and can rapidly catalyze hydrogen peroxide to oxidize cysteine, experimental results show that the catalytic process only needs several minutes and has remarkable advantages compared with literature records, and due to the fact that the optimal pH range is slightly neutral, the catalytic process is irrelevant to a Fenton reagent, and the kit belongs to a novel gold nano color development system; and according to the kit, corresponding compounds in a sample to be detected are oxidized by oxidase to generate H2O2, the concentration of the compounds in the sample to be detected is further calculated, qualitative and quantitative detection of multiple compounds such as lactic acid, glucose, cholesterol and uric acid can be achieved, and the kit has huge application value.

Description

technical field [0001] The invention relates to the fields of analytical chemistry and biological detection, in particular to a kit, a method for using the same, and a method for detecting the concentration of hydrogen peroxide. Background technique [0002] The synthesis of gold nanometers is simple, the material is stable, the particle size effect is significant, the color is visible to the naked eye, and it is easy to be derivatized and modified. It has sufficient development prospects and great application value. Gold nanometers can be widely used in various occasions such as visible color development, signal amplification, and fluorescence analysis, among which gold nanometer color development technology is the most common application. The color development of gold nanoparticles is based on the change of the size of the nanoparticles, which produces a color change. Small-size gold nanoparticles (10-20nm) appear wine red, while large-size gold nanoparticles (>100nm) c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/31
CPCG01N21/78G01N21/31G01N2021/775
Inventor 郑军平刘洪涛刘雨方海田叶诚张聪卞庆来
Owner HUBEI UNIV OF CHINESE MEDICINE
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