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Method for quantitatively detecting soluble beta amyloid protein by using ratiometric type fluorescent probe

An amyloid protein and fluorescent probe technology, which is applied in the field of medicine and achieves good effects, easy preparation and good selectivity.

Inactive Publication Date: 2019-06-28
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the application of ratiometric small molecule fluorescent probes for the quantitative detection of soluble Aβ aggregates and the development of AD diagnostic agents has not been found so far.

Method used

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  • Method for quantitatively detecting soluble beta amyloid protein by using ratiometric type fluorescent probe
  • Method for quantitatively detecting soluble beta amyloid protein by using ratiometric type fluorescent probe
  • Method for quantitatively detecting soluble beta amyloid protein by using ratiometric type fluorescent probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0026] Example: Quantitative detection of soluble Aβ oligomers by the ratiometric fluorescent probe BPNS:

[0027] Step 1: Synthesis of the preferred ratiometric fluorescent probe BPNS, the synthesis route is shown below.

[0028]

[0029] Weigh 2-aminobenzenethiol (1 mmol, 0.125 g), p-aminosalicylic acid (1 mmol, 0.138 g) and 50 ml of polyphosphoric acid (PPA) in a three-neck round bottom flask. React at 130°C for 120 minutes. When the reaction time is up, add water to stop the reaction. The system is a clear bright yellow solution with a pH value of 1. Add saturated sodium hydroxide solution to adjust the pH to neutral. Bright yellow powder precipitates. Filter and collect the filter residue , washed three times with water (3×100 mL). The crude product was separated by a silica gel column (PE:EA=4:1) to finally obtain Compound 1 (0.184 g, yield: 70%) as a bright yellow powder. 1 H NMR (CDCl 3 , 400MHz, δ, ppm): 8.16 (d, J=8.5Hz, 1H, benzothiazole), 8.12 (d, J=2.6Hz, 1H...

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Abstract

The invention discloses a method for quantitatively detecting soluble beta amyloid protein (Abeta) by using a ratiometric type fluorescent probe and belongs to the technical field of medicines. According to the method disclosed by the invention, a metal ion ratiometric type fluorescent probe BPNS is obtained by coupling 2-pyridyl benzothiazole with a dansyl fluorophore. The method has the beneficial effects that the soluble Abeta has a high affinity for Zn<2+> or Cu<2+> and is capable of separating the metal ions from BPNS-metal ion adducts through chelation to trigger the ratiometric fluorescence response of the BPNS; the accurate quantitative detection of soluble Abeta aggregates can be realized by determining the linear relationship between the BPNS dual-wavelength fluorescence intensity ratio and the concentration of the soluble Abeta aggregates; and the method has a very good application prospect in the early diagnosis of AD.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a method for quantitatively detecting beta amyloid protein with a ratiometric fluorescent probe. Background technique [0002] Alzheimer's disease (AD) is a progressive neurodegenerative disease, clinically mainly manifested as memory and cognitive impairment, and there is currently no effective diagnosis and treatment. Although the pathological mechanism of AD is still unclear, a large number of studies have shown that the main pathological features of AD are senile plaques (SP) formed by the aggregation and deposition of β-amyloid protein (Aβ) and neurofibrillary tangles formed by the aggregation of hyperphosphorylated Tau protein. (NFTs). Among them, Aβ aggregation is considered to be the core etiology in the development of AD. Aβ is a polypeptide composed of 39-43 amino acids, which is produced by the hydrolysis of amyloid precursor protein by β- and γ-secretas...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 王小辉李长洪杨柳
Owner NANJING UNIV OF TECH
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