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Ratiometric fluorescence biosensor for detecting aflatoxin-producing genes and preparation of ratiometric fluorescence biosensor

A biosensor, aflatoxin technology, applied in the field of fluorescence sensing, can solve the problems of limiting the application of single-wavelength fluorescence sensing technology, and achieve the effects of rapid detection, high optical stability, and large specific surface area

Pending Publication Date: 2021-04-16
JIANGSU UNIV OF SCI & TECH
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Problems solved by technology

This single detection mode of fluorescence signal change is easily affected by the detection environment and the sample itself, and requires strict control of detection conditions to obtain more accurate detection results, which limits the application of single-wavelength fluorescence sensing technology in detection

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  • Ratiometric fluorescence biosensor for detecting aflatoxin-producing genes and preparation of ratiometric fluorescence biosensor

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Embodiment Construction

[0045] The preferred embodiments of the present invention are described in detail below, so that the advantages and features of the present invention can be more easily understood by those skilled in the art, so as to define the protection scope of the present invention more clearly.

[0046] In order to improve the detection efficiency and detection accuracy of aflatoxin, and to control the diffusion and pollution of aflatoxin from the source, this embodiment discloses a ratiometric fluorescent biosensor for detecting aflatoxin-producing genes. The specific preparation process is as follows:

[0047] 1) Preparation of gold nanoparticles AuNPs

[0048] A certain amount of chloroauric acid solution with a concentration of 200 μM was put into the flask, heated to vigorous boiling under stirring, and 0.4 mL of sodium citrate solution with a concentration of 350 mM was added into the boiling chloroauric acid solution under vigorous stirring, When the color of the solution changed ...

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Abstract

The invention discloses a ratiometric fluorescence biosensor for detecting aflatoxin-producing genes as well as preparation and application of the ratiometric fluorescence biosensor. A fluorescence energy donor of the fluorescence biosensor is a CQDsI-coated-SiO2-coated CQDsII hybrid nanosphere with a three-layer coating structure, a hairpin structure DNA sequence with two ends respectively modified with -SH and -NH2 is selected as a capture probe, and an energy receptor is AuNPs; when no toxin-producing gene appears, the AuNPs and the CQDsII are close in spatial distance, and FRET is triggered, so that fluorescence quenching of the CQDsII is caused; and when a toxin-producing gene exists, the toxin-producing gene is specifically paired with the fluorescent probe, so that the energy resonance transfer is blocked, the fluorescence of the CQDsII is recovered, and the fluorescence emission characteristic of the CQDsI is not changed due to the protection of the SiO2 nanospheres in the middle layer. The ratiometric fluorescent probe can provide a new thought for rapidly detecting aflatoxin toxin producing genes from the source.

Description

technical field [0001] The invention relates to the technical field of fluorescent sensing, in particular to a ratio fluorescent biosensor for detecting aflatoxin toxin-producing genes and its preparation and application. Background technique [0002] Aflatoxin (AF) is a secondary metabolite produced by toxin-producing strains such as Aspergillus flavus and Aspergillus parasiticus, which is extremely toxic to humans and can cause significant damage to the liver, causing liver parenchyma necrosis, Epithelial hyperplasia, hepatic fatty infiltration and liver hemorrhage and other lesions, and can cause the loss of immune function of the human body or animals, and can induce deformity and cancer. Among naturally contaminated foods, aflatoxin B1 (AFB1) is the most common and one of the most powerful chemical carcinogens known. In 1993, the Cancer Research Institute of the World Health Organization (WHO) classified AFB1 as a Class I carcinogen (for humans). The toxicity of AFB1 i...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6825G01N21/64
Inventor 李雅琪余涛李超臧清露孔德昭刘畅史巧巧
Owner JIANGSU UNIV OF SCI & TECH
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