Human myocardium protecting gene and uses thereof

A kind of myocardial protection and gene technology, applied in the direction of gene therapy, genetic engineering, plant genetic improvement, etc., to achieve the effect of reversing myocardial death and protecting myocardium

Inactive Publication Date: 2008-06-18
ZHONGSHAN HOSPITAL FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no research on the application of death trap gene screening method to heart disease

Method used

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  • Human myocardium protecting gene and uses thereof
  • Human myocardium protecting gene and uses thereof
  • Human myocardium protecting gene and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Use the death trap method to screen the genes that protect the myocardium against hypoxic injury

[0029] Rat cardiac fibroblasts were used as the research object, and human heart gene library (pCMVSPORT heart cell expression cDNA library) was transfected by electroporation. After 48 hours, the transfected cells were stimulated with lethal hypoxia to induce cell death, and the surviving cell clones may contain genes that protect the myocardium against hypoxic injury. The subgene pool was extracted from the surviving cell clones and then transfected into cardiac fibroblasts, and the experimental procedure was repeated 4 times. The most obviously altered genes were screened from the last surviving cell clones, and their cDNAs were cloned.

[0030] 2. Verify whether the screened cDNA has cardioprotective effect and optimize its interaction mode

[0031] 1. Cytological level

[0032] The cloned cDNA was transfected into cardiomyocytes respectively, and after giving le...

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Abstract

The present invention belongs to the biotechnological field and relates to a human myocardial protection gene heat shock transcription factor (HSF1) and a sieving method and a purpose thereof. The present invention adopts the method of ''death trap'' that the gene which resists myocardial cell death is sieved from human cardiac gene, and the human myocardial protection gene heat shock transcription factor 1 is cloned from the obtained gene. The result of transfection cell and animal experiment shows that the obtained human myocardial protection gene has the function of resisting cell death and also has the function of resisting ischemia, protecting myocardial cell and preventing and remedying the heart failure occurrence and development inside the heart of a living organism. The present invention can prepare for the medicine for remedying the ischemic heart disease and preventing the cardiac insufficiency and provides a novel target gene for the further function research and the gene engineering medicine development, which has important meaning for the early prevention and treatment of the heart failure and the research and the development of the related medicines and measures.

Description

technical field [0001] The invention belongs to the field of biological technology and relates to a human cardioprotective gene, in particular to a human cardioprotective gene heat shock transcription factor (HSF1) and its screening method and use. Background technique [0002] Heart failure is a group of complex clinical syndromes, which is the severe stage of most patients with organic heart disease, and the 5-year survival rate is similar to that of malignant tumors. Once heart failure occurs in heart disease patients, the 1-year mortality rate is 43%, and the 5-year mortality rate reaches 75%, seriously endangering human health. Epidemiological data show that the number of heart failure patients in the world is as high as 22.5 million, and there are 2 million new cases every year. The rapid aging of the population and the increase of various risk factors make ischemic heart disease the most common cause of heart failure. In developed countries, the increase in the survi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12A61K48/00A61P9/04A61P9/10
Inventor 邹云增姜红李纪明张磊
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
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