8 results about "RNA Aptamers" patented technology

The present invention relates to a probe set for an isothermal single reaction using a split T7 promoter and a use thereof. In the present invention, a design is made to introduce a split T7 promoter into a 3-way junction structure to produce a large quantity of fluorescent RNA aptamers at an isothermal temperature in the presence of a target molecule, and a nucleic acid biomarker can be detected rapidly and conveniently with only one enzyme in one pot. The probe set allows for various applications through high-sensitivity detection of multiple nucleic acid biomarkers and detection in which analysis is performed without an additional nucleic acid extraction process. In one pot, multiplex analysis can be conducted and molecular diagnosis can be made of various entities including viruses and pathogens.

The present invention relates to an in vivo method for comparing the specific plasmid titers in two or more bacterial cell populations, using RNA aptamers.

The invention provides a method for screening a targeted binding RNA molecule by using a fluorescent RNA aptamer, and particularly, the method comprises the following steps: fusing the fluorescent RNA aptamer and a target RNA to obtain a fused RNA, co-incubating the fused RNA, a fluorescent agent and a molecule to be detected, then carrying out fluorescence measurement, and screening the molecule targeting the target RNA according to fluorescence change.

Compositions and methods for amplifying RNA by replication using transcription polymerases are disclosed. Such replicated RNAs can be used in various applications such as RNAi therapeutics, diagnostic probes, RNA sequencing, directed evolution of RNA aptamers without intermediate conversion to DNA, and RNA vaccines. The transcription polymerases comprise T7 bacteriophage RNA polymerase.

Described herein and RNA aptamers that specifically bind to tumor-infiltrating myeloid cells and uses thereof.

The invention relates to single-stranded DNA or RNA aptamers and uses thereof. Pharmaceutical compositions and kits comprising such aptamers and uses thereof in the medical field, in particular in the treatment of cancer, are also described.

The invention relates to a fluorescent RNA (Ribonucleic Acid) functionalized hydrogel kit, a preparation method and a method for detecting colorectal cancer miRNA-126, solves the technical problem of how to detect miRNA-126 in blood or tissue samples and is suitable for portable detection, and fluorescent RNA functionalized hydrogel is prepared from methacrylic acid hyaluronic acid, an acryloyl-modified fluorescent RNA aptamer and N, N-dimethylformamide. The fluorescent RNA aptamer is obtained by crosslinking the fluorescent RNA aptamer and N, N '-methylene bisacrylamide, and the covalent crosslinking between the fluorescent RNA aptamer and the hydrogel can prevent the RNA aptamer from being released into the external environment, so that the stability and the anti-interference capability of the hydrogel kit are improved.

Disclosed herein is a method of identifying an L-RNA aptamer specific for a target nucleic acid. According to some embodiments of the present disclosure, the method comprises, in vitro transcribing the DNAs of the DNA library into D-RNAs, followed by identifying target-specific D-RNAs via negative and positive selections, and then producing the L-RNA aptamer based on the identified D-RNA. Also disclosed herein are two aptamers identified by the present method. According to some embodiments of the present disclosure, the two aptamers respectively comprise the nucleotide sequences of SEQ ID NOs: 1 and 2.