A kind of production method and application of recombinant small RNA

A production method and sequence technology, applied in the fields of molecular biology and medicine, can solve the problems of limited application scope, inability to express a variety of small RNAs, etc., achieve low toxicity and immunogenicity, improve complementary pairing conditions, functional Good results
CN108753780BActive Publication Date: 2022-03-29西安荣清畅生物科技有限公司 +1
2 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
西安荣清畅生物科技有限公司
Publication Date
2022-03-29

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a production method of recombinant small RNA. In the method, tRNA is used as a scaffold, and a chimeric miRNA precursor is used to express target small RNA in Escherichia coli. In addition, the invention also discloses the application of the recombinant small RNA produced by the method. The recombinant small RNA designed and prepared by the present invention is obtained through bioengineering technology, and has the advantages of simple and convenient equipment, rapid production, high yield, low cost, and good functionality; tests by various technical means show that the method of the present invention can conveniently and quickly Prepare a variety of miRNA / siRNA / RNA aptamers, and produce recombinant small RNAs to meet the needs of scientific research and drug development.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the field of molecular biology and medical technology, and specifically relates to a production method and application of recombinant small RNA. Background technique

[0002] Noncoding RNA (noncoding RNA, ncRNA) has broad application prospects in the research of biological related fields and disease treatment. In the past two decades, small ncRNAs have been well established as key epigenetic factors of gene regulation. These small ncRNAs mainly participate in the formation of the silencing complex (RISC) through the RNAi pathway, thereby inducing gene silencing. The research on the function of ncRNA has given birth to a new treatment method, that is, the birth of RNA therapy. For example, reconstitution of tumor suppressor miRNA levels can significantly slow down tumor disease progression.

[0003] However, the research on the function, pharmacological activity and therapeutics of ncRNA depends on the acquisition of relevant...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More