Arabidopsis thaliana heat shock transcription factor gene HSFA3, encoding protein and applications thereof

A heat shock transcription factor, Arabidopsis technology, applied in the fields of plant molecular biology and transgenics, can solve the problems of increasing gene function, and at a loss for theoretical and technical research.

Inactive Publication Date: 2013-08-21
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
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Problems solved by technology

But so far there is no report on the heat stress response of the HSFA3 gene, which makes it difficult for people

Method used

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  • Arabidopsis thaliana heat shock transcription factor gene HSFA3, encoding protein and applications thereof
  • Arabidopsis thaliana heat shock transcription factor gene HSFA3, encoding protein and applications thereof
  • Arabidopsis thaliana heat shock transcription factor gene HSFA3, encoding protein and applications thereof

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Embodiment Construction

[0014] The present invention will be further described below in conjunction with experimental methods and data.

[0015] 1. Arabidopsis seedling culture:

[0016] Wild-type and mutant seeds were sterilized by soaking in 0.5% sodium hypochlorite solution for 10 min, and the sterilized seeds were placed in a 4°C refrigerator for vernalization for 2 days, and then spread on MS (Murashige and Skoog) solid medium containing 1% agar. Germinate and grow in a light incubator at 22°C.

[0017] 2. Homozygous mutant identification:

[0018] DNA extraction: All experimental drugs used were purchased from Zhongke Ruitai Bioengineering Co., Ltd. Take about 0.5g fresh leaves of Arabidopsis thaliana, grind them into a paste, add 400μl SDS extract (0.5% SDS (W / V); 200mM Tris-HCl, pH=8.0; 250mM NaCl; 25mM EDTA); centrifuge at 12,000r / min 3min; take the supernatant, add an equal volume of isopropanol to gently shake, let stand for 30min; centrifuge at 13,000r / min for 5min; discard the superna...

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Abstract

The invention belongs to the plant molecule biology and transgenic related technical field, and specifically relates to verification of high temperature resistance of arabidopsis thaliana heat shock transcription factor gene HSFA3 and applications of the gene in breeding of new high temperature resistance varieties. According to the present invention, a model plant arabidopsis thaliana is adopted as a material, and HSFA3 gene T-DNA is adopted to insert into a homozygosis mutant line salk_011131 so as to research functions of the HSFA3 gene in the field of high temperature resistance of plants, wherein experiment results show that the HSFA3 gene knocked out mutant presents a heat resistance phenotype under heat shock stress, and a root length and above-ground biomass of the HSFA3 gene knocked out mutant are significantly more than a root length and above-ground biomass of the wild type, such that the HSFA3 gene provides important effects in a signal transduction pathway of the plant response to heat shock stress, and it can be predicted that overexpression of the gene can increase heat resistance of the plant and can provide high economic values in plant stress tolerance molecule breeding.

Description

Technical field: [0001] The invention relates to the field of plant molecular biology and transgenic technology, specifically the verification of the new function of HSFA3, a member of the Arabidopsis thaliana heat shock transcription factor (Heat Shock Transcription Factors, HSFs) gene family, for high temperature resistance, and the application of the gene in new high temperature resistant plant varieties applications in cultivation. Background technique: [0002] The change of temperature has an important impact on the growth and development of plants. Appropriate temperature can ensure or promote the normal growth and development of plants, while unsuitable temperature conditions will inhibit the growth of plants and even cause plant death. Since the 20th century, with the rapid growth of world population and the continuous deepening of industrialization, the greenhouse effect has become more and more serious, and the gradual increase of global temperature has become an ...

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Application Information

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IPC IPC(8): C12N15/29C07K14/415A01H5/00
Inventor 徐江徐小栋东方阳鲁黎明王西瑶张少斌迟志海洪雪麻艳超赵明丁在松付金东
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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