Application of ZmMAPK4 gene in enhancing cold resistance of plants
By cloning and constructing the ZmMAPK4 gene and overexpressing it in maize, the problem of insufficient tolerance of maize to low temperature stress was solved, and the cold resistance of maize was significantly enhanced, providing genetic resources and breeding guidance.
Patent Information
- Application Number
- CN202511140044.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-08-14
- Publication Date
- 2025-11-28
AI Technical Summary
Existing technologies are insufficient to effectively enhance maize's tolerance to low-temperature stress in breeding. Traditional breeding techniques suffer from long cycles and low efficiency. Furthermore, there is a lack of research on whether other members of the maize ZmMAPK family respond to low-temperature stress and whether there are positive regulatory factors involved in the cold stress response.
The ZmMAPK4 gene was cloned and constructed. The gene was overexpressed or knocked down in maize through genetic engineering. Maize cells were then transformed using Agrobacterium-mediated transformation to obtain transgenic plants with enhanced cold resistance.
It significantly enhanced the cold tolerance of maize, reduced the leaf damage area and ion leakage rate under low temperature stress, and demonstrated the role of the ZmMAPK4 gene in enhancing plant cold tolerance.
Smart Images
Figure CN121022909A_ABST