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Application of piriformospora indica in regulating drought tolerance of osmanthus fragrans

An Indian piriformis and drought-tolerant technology, which is applied in the field of drought-tolerant osmanthus cultivation, can solve the problems of unfruitful osmanthus and immature in vitro regeneration system of osmanthus, and achieve the effect of easy mass production and improved tolerance

Pending Publication Date: 2020-12-15
YANGTZE UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

More than half of the varieties of sweet-scented osmanthus are not fruiting, and the in vitro regeneration system of sweet-scented osmanthus is not yet mature, which brings great resistance to the realization of germplasm innovation and the cultivation of new varieties

Method used

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  • Application of piriformospora indica in regulating drought tolerance of osmanthus fragrans
  • Application of piriformospora indica in regulating drought tolerance of osmanthus fragrans
  • Application of piriformospora indica in regulating drought tolerance of osmanthus fragrans

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Effect of inoculation of Pyromorpha indica on the growth of osmanthus fragrans under the drought stress condition of embodiment 1

[0024] Using 2-year-old osmanthus seedlings as materials, select osmanthus annual seedlings with a plant height of about 21cm, mix the sterilized and cooled culture medium and sterilized soil 1:1 as culture soil (2.5kg per pot), place In a gallon pot (diameter 18cm, height 20cm), a plastic tray is placed on the bottom of the pot to prevent water and fertilizer loss. At the beginning of May 2019, the osmanthus seedlings were transplanted into pots, and Piriformospora indica was applied, and the inoculation amount was 0.4g mycelium / kg soil. Drought stress and control experiments will start in mid-May 2019, specifically:

[0025] Two experimental factors of inoculation and drought were set, and the drought treatment was set up with drought (maintaining soil water content as 15% of the field maximum water capacity), moderate water supply (main...

Embodiment 2

[0031] Example 2 Inoculation of Pyromorpha indica affects the physiological and biochemical indicators of sweet-scented osmanthus

[0032] The settings of the drought stress and control experiments were the same as in Example 1, that is, there were 6 treatment combinations, which were: inoculation+15% water, non-inoculation+15% water, inoculation+45% water, non-inoculation+45% water, inoculation +75% water, not inoculated +75% water.

[0033] Determination of physiological and biochemical indicators related to drought resistance of osmanthus seedlings, including: superoxide dismutase (SOD), peroxidase (POD), malondialdehyde (MDA), free proline (SP), soluble sugar (SS ). Superoxide dismutase is an important antioxidant enzyme in organisms, and its activity is determined by nitrogen blue tetrazolium (NBT) photoredox method; peroxidase is a heme protein composed of a single peptide chain and porphyrin , is widely present in plants, catalyzes the oxidation reaction of various re...

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Abstract

The invention discloses an application of piriformospora indica in regulating drought tolerance of osmanthus fragrans. That is, in a cultivation process, the piriformospora indica or a biological agent containing the piriformospora indica is inoculated in osmanthus fragrans seedlings, so that the piriformospora indica and osmanthus fragrans root systems are symbiotic, the drought tolerance of theosmanthus fragrans to soil drought can be stimulated, and the drought tolerance of the osmanthus fragrans is remarkably improved. The normal growth increment can be still maintained under the water supply condition that the maximum field water-holding capacity of the osmanthus fragrans is 15%, meanwhile, the content of soluble sugar and free proline and the activity of POD and SOD enzymes in osmanthus fragrans leaves are remarkably improved, and the content of malondialdehyde is remarkably reduced. Therefore, a new thought and a technical basis are provided for water-saving cultivation of osmanthus fragrans. The piriformospora indica can be rapidly proliferated by utilizing an artificial culture medium, is easy to produce in batches, is further popularized and applied on a large scale, andhas commercial potential advantages.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation and planting, and in particular relates to a drought-tolerant cultivation method of sweet-scented osmanthus. Background technique [0002] Drought is one of the three major natural disasters that limit plant production, causing the most severe losses to agricultural production and ecosystems. my country has about 3 trillion cubic meters of water storage, and it ranks behind in the per capita water resource share in the world. Moreover, the uneven distribution of water resources in terms of geographical location and seasonal distribution also affects agricultural and forestry production. bring challenges. Insufficient utilization of water in agricultural and forestry production in my country has resulted in a large waste of water resources. It can be seen that my country's water resources are facing a very severe situation, and it is imperative to explore new methods of water-saving culti...

Claims

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Application Information

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IPC IPC(8): A01G7/06A01G17/00A01G24/20
CPCA01G7/06A01G17/005A01G24/20
Inventor 母洪娜孙陶泽
Owner YANGTZE UNIVERSITY
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