Method for improving drought resistance of oil Paeonia suffruticosa Andr. by adopting methyl jasmonate

Abstract

The invention discloses a method for improving the drought resistance of oil Paeonia suffruticosa Andr. by adopting methyl jasmonate. The methyl jasmonate is adopted to induce the oil Paeonia suffruticosa Andr. to generate the drought resistance. The method comprises the following steps that 1), triennial oil Paeonia suffruticosa Andr. paeonia ostii seedlings which are consistent in growth vigor and free of plant diseases and insect pests are selected in October to be cultivated; 2), the paeonia ostii seedlings are cultured at room temperature until May of the next year, and water management is carried out during the period; and 3), the methyl jasmonate is sprayed to the oil Paeonia suffruticosa Andr. once every 3 days, and the methyl jasmonate is sprayed twice in total, and the drought-resistant oil Paeonia suffruticosa Andr. is obtained. According to the method for improving the drought resistance of the oil Paeonia suffruticosa Andr. by adopting methyl jasmonate, after the methyl jasmonate is adopted for induction treatment, the net photosynthetic rate, the transpiration rate, the intercellular CO2 concentration, the stomatal conductance, the free proline content and the solublesugar content in leaves are in the increase, the malondialdehyde content, the chlorophyll a content, the chlorophyll b content and the carotenoid content are in the decrease, the net photosynthetic rate of the paeonia ostii leaves is increased, the damage of drought stress to the Paeonia suffruticosa Andr. is reduced, and the drought stress resistance of the paeonia ostii is enhanced.

Description

technical field

[0001] The invention relates to the technical field of peony planting, in particular to a method for improving the drought resistance of peony for oil by using methyl jasmonate. Background technique

[0002] Peony (Paeoniasuffruticosa Andr.) is a perennial deciduous shrub of the family Paeoniaceae. It is a traditional famous flower in my country and is known as the "king of flowers". The Heluo area is rich in peony resources and is one of the original places of wild peonies in China. In recent years, peony-related industries have developed rapidly, and its deep-processed products are widely used in ornamental, oily, edible and medicinal fields. Peony for oil is the collective name of 'Paeoniaostii' (Paeoniaostii) and 'Purple Spot' (Paeoniarockii) peonies with high seed setting rate and high oil yield rate in peony species, and it is a unique emerging woody oil crop in my country. Peony seed oil is a golden yellow transparent oily liquid, rich in linolenic a...

Examples

Embodiment 1

[0027] Step 1: Select three-year-old oil peony 'Fengdan' plants with consistent growth and no pests and diseases, and plant them in plastic flower pots with a diameter of 20 cm and holes at the bottom. The substrate is the original soil from the farm of Henan University of Science and Technology.

[0028] Step 2: After planting, move it to the greenhouse for maintenance, and carry out normal water management to prevent water shortage and prevent the occurrence of diseases and insect pests.

[0029] Step 3: Use the whole plant spraying method, use 50 μmol / L methyl jasmonate to induce treatment, preferably drip water on the upper and lower surfaces of the leaves, and set 5 repetitions for each treatment.

[0030] Step 4: After 3 days of treatment with methyl jasmonate, carry out gradual drought stress treatment, during which observation and analysis of phenotypic characteristics changes are carried out every day, and according to the changes in the phenotypic characteristics of t...

Embodiment 2

[0033] Step 1: Select three-year-old oil peony 'Fengdan' plants with consistent growth and no pests and diseases, and plant them in plastic flower pots with a diameter of 20 cm and holes at the bottom. The substrate is the original soil from the farm of Henan University of Science and Technology.

[0034] Step 2: After planting, move it to the greenhouse for maintenance, and carry out normal water management to prevent water shortage and prevent the occurrence of diseases and insect pests.

[0035] Step 3: Use the whole plant spraying method, use 100 μmol / L methyl jasmonate for induction treatment, preferably drip water on the upper and lower surfaces of leaves, and set 5 repetitions for each treatment.

[0036] Step 4: After 3 days of treatment with methyl jasmonate, carry out gradual drought stress treatment, during which observation and analysis of phenotypic characteristics changes are carried out every day, and according to the changes in the phenotypic characteristics of ...

Embodiment 3

[0039] Step 1: Select three-year-old oil peony 'Fengdan' plants with consistent growth and no pests and diseases, and plant them in plastic flower pots with a diameter of 20 cm and holes at the bottom. The substrate is the original soil from the farm of Henan University of Science and Technology.

[0040] Step 2: After planting, move it to the greenhouse for maintenance, and carry out normal water management to prevent water shortage and prevent the occurrence of diseases and insect pests.

[0041] Step 3: use the whole plant spraying method, use 200 μmol / L methyl jasmonate for induction treatment, preferably drip water on the upper and lower surfaces of leaves, and set 5 repetitions for each treatment.

[0042] Step 4: After 3 days of treatment with methyl jasmonate, carry out gradual drought stress treatment, during which observation and analysis of phenotypic characteristics changes are carried out every day, and according to the changes in the phenotypic characteristics of ...

technical field

[0001] The invention relates to the technical field of peony planting, in particular to a method for improving the drought resistance of peony for oil by using methyl jasmonate. Background technique

[0002] Peony (Paeoniasuffruticosa Andr.) is a perennial deciduous shrub of the family Paeoniaceae. It is a traditional famous flower in my country and is known as the "king of flowers". The Heluo area is rich in peony resources and is one of the original places of wild peonies in China. In recent years, peony-related industries have developed rapidly, and its deep-processed products are widely used in ornamental, oily, edible and medicinal fields. Peony for oil is the collective name of 'Paeoniaostii' (Paeoniaostii) and 'Purple Spot' (Paeoniarockii) peonies with high seed setting rate and high oil yield rate in peony species, and it is a unique emerging woody oil crop in my country. Peony seed oil is a golden yellow transparent oily liquid, rich in linolenic a...

Examples

Embodiment 1

[0027] Step 1: Select three-year-old oil peony 'Fengdan' plants with consistent growth and no pests and diseases, and plant them in plastic flower pots with a diameter of 20 cm and holes at the bottom. The substrate is the original soil from the farm of Henan University of Science and Technology.

[0028] Step 2: After planting, move it to the greenhouse for maintenance, and carry out normal water management to prevent water shortage and prevent the occurrence of diseases and insect pests.

[0029] Step 3: Use the whole plant spraying method, use 50 μmol / L methyl jasmonate to induce treatment, preferably drip water on the upper and lower surfaces of the leaves, and set 5 repetitions for each treatment.

[0030] Step 4: After 3 days of treatment with methyl jasmonate, carry out gradual drought stress treatment, during which observation and analysis of phenotypic characteristics changes are carried out every day, and according to the changes in the phenotypic characteristics of t...

Embodiment 2

[0033] Step 1: Select three-year-old oil peony 'Fengdan' plants with consistent growth and no pests and diseases, and plant them in plastic flower pots with a diameter of 20 cm and holes at the bottom. The substrate is the original soil from the farm of Henan University of Science and Technology.

[0034] Step 2: After planting, move it to the greenhouse for maintenance, and carry out normal water management to prevent water shortage and prevent the occurrence of diseases and insect pests.

[0035] Step 3: Use the whole plant spraying method, use 100 μmol / L methyl jasmonate for induction treatment, preferably drip water on the upper and lower surfaces of leaves, and set 5 repetitions for each treatment.

[0036] Step 4: After 3 days of treatment with methyl jasmonate, carry out gradual drought stress treatment, during which observation and analysis of phenotypic characteristics changes are carried out every day, and according to the changes in the phenotypic characteristics of ...

Embodiment 3

[0039] Step 1: Select three-year-old oil peony 'Fengdan' plants with consistent growth and no pests and diseases, and plant them in plastic flower pots with a diameter of 20 cm and holes at the bottom. The substrate is the original soil from the farm of Henan University of Science and Technology.

[0040] Step 2: After planting, move it to the greenhouse for maintenance, and carry out normal water management to prevent water shortage and prevent the occurrence of diseases and insect pests.

[0041] Step 3: use the whole plant spraying method, use 200 μmol / L methyl jasmonate for induction treatment, preferably drip water on the upper and lower surfaces of leaves, and set 5 repetitions for each treatment.

[0042] Step 4: After 3 days of treatment with methyl jasmonate, carry out gradual drought stress treatment, during which observation and analysis of phenotypic characteristics changes are carried out every day, and according to the changes in the phenotypic characteristics of ...

Images