Solvent for improving antioxidation metabolic capacity of flue-cured tobacco

A metabolic ability and anti-oxidation technology, applied in biocides, animal repellents, plant preservation, etc., can solve problems that do not involve the antioxidant and metabolic properties of tobacco leaves, so as to reduce adverse effects, maintain normal growth and development, and ensure production and the effect on output value

Active Publication Date: 2013-07-24
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current research mostly involves tobacco water retention and growth promotion, but does not involve the antioxidant and metabolic properties of tobacco leaves

Method used

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  • Solvent for improving antioxidation metabolic capacity of flue-cured tobacco
  • Solvent for improving antioxidation metabolic capacity of flue-cured tobacco
  • Solvent for improving antioxidation metabolic capacity of flue-cured tobacco

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Solvent preparation: the weight percent of each component of the solvent in this embodiment is: betaine 0.25%, proline 0.10%, nano carbon sol 0.03%, and the rest is water.

[0017] Application time: In the early stage of tobacco plant growth, it is divided into 3 times of use, with an interval of 3 days between each time. From the top of the tobacco plant, spray the solvent evenly on the front and back of the tobacco leaves with a sprayer from top to bottom.

[0018] Each treatment in the test was sprayed three times in a row, and then the water supply was stopped, and the drought stress treatment was started. Fresh tobacco leaf samples in the middle of the tobacco plant were collected 3, 6, and 9 days after drought stress, respectively, and the activities of SOD, POD, and CAT were determined, as well as the contents of Pro and MDA.

[0019] Specific implementation effect:

[0020] As can be seen from Table 1, compared with the clear water contrast, after spraying the...

Embodiment 2

[0028] Repeat Example 1, the following difference is arranged: the weight percent of each component of present embodiment solvent is: betaine 0.50%, proline 0.05%, nano carbon sol 0.01%, all the other are water.

[0029] Application time: In the early stage of tobacco plant growth, it is divided into 2 times of use, with an interval of 5 days between each time.

[0030] Specific implementation effect:

[0031] It can be seen from Table 3 that compared with the clear water control, after spraying the solvent of the present invention, the SOD, POD and CAT activities of the flue-cured tobacco leaves were significantly improved, effectively improving the antioxidant metabolism of the tobacco leaves.

[0032] Table 3 Changes of antioxidant metabolic enzymes in flue-cured tobacco after spraying solvent

[0033]

[0034] Table 4 finds that compared with the clear water contrast, after spraying the solvent of the present invention, the content of proline (Pro) in flue-cured tobacc...

Embodiment 3

[0038] Repeat Example 1, the following difference is arranged: the weight percent of each component of the present embodiment solvent is: betaine 0.10%, proline 0.25%, nano carbon sol 0.05%, all the other are water.

[0039] Application time: In the early stage of tobacco plant growth, it is divided into 3 times of use, with an interval of 5 days between each time.

[0040] Specific implementation effect:

[0041] It can be seen from Table 5 that compared with the clear water control, after spraying the solvent of the present invention, the SOD, POD and CAT of the flue-cured tobacco leaves increased by 7.87%, 16.72% and 14.17% on average respectively, effectively improving the antioxidant metabolism of the tobacco leaves.

[0042] Table 5 Changes of antioxidant metabolic enzymes in flue-cured tobacco after spraying solvent

[0043]

[0044] Table 6 finds that, compared with the clean water contrast, after spraying the solvent of the present invention, the content of prolin...

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Abstract

The invention discloses a solvent for improving the antioxidation metabolic capacity of flue-cured tobacco. The solvent is characterized by being prepared from the following ingredients by weight percent: 0.1-0.5% of betaine, 0.05-0.25% of proline, 0.01-0.05% of nano carbon sol and the balance of water. The application method of the solvent is as follows: the solvent is used for 2-3 times in the early flourishing stage of a tobacco plant and interval time between two times of applications is 3-5 days; and the solvent is uniformly sprayed on the front face and the back face of a tobacco leaf by a sprayer, and the spraying reaches the effect that the leaf face is wet and water does not drop from the leaf. The solvent is based on the antioxidation metabolism characteristic of the tobacco leaf, the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) of the tobacco leaf can be improved, the content of the important antioxidation metabolic substance, namely proline (Pro) is increased, and the content of membranous maleicdialdehyde (MDA) in the tobacco leaf is reduced. The experiments prove that the spraying of the solvent disclosed by the invention can effectively reduce the adverse effect of drought stress on the flue-cured tobacco, the normal growth and development of the tobacco plant can be maintained, and the yield and output value of the tobacco leaf can be ensured.

Description

[0001] technical field [0002] The invention belongs to the cultivation and production technology of tobacco leaves in the field of tobacco agriculture, and in particular relates to a solvent for improving the antioxidant capacity of tobacco leaves and an application method thereof. [0003] Background technique [0004] During the growth and development of plants, they are often affected and stressed by various adverse environments. The main feature of plants subjected to adversity stress is the imbalance of active oxygen metabolism, which produces superoxide anion free radicals (O 2 - ), hydrogen peroxide (H 2 o 2 ) and hydroxyl radicals (·OH-) and other reactive oxygen species (reactive oxygen species, ROS) substances. Under normal circumstances, plant cells can keep the production and removal of ROS in plants in a state of dynamic balance through multiple pathways, without causing damage to plants. However, under adversity conditions, the dynamic balance between t...

Claims

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Application Information

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IPC IPC(8): A01N59/00A01P21/00A01N3/00A01N37/44A01N43/36
Inventor 梁太波尹启生张艳玲王建伟薛超群刘阳宋纪真过伟民王广山
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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