LncRNA and application thereof

Abstract

The invention provides a gene corresponding to LncRNA. The nucleotide sequence of the gene is shown in SEQ ID NO: 1. The invention also provides a transcribed LncRNA corresponding to the gene and application of the LncRNA. The gene corresponding to the LncRNA and the LncRNA transcribed by the gene can specifically respond to low-temperature stress response and drought stress response. By further transferring the gene into cassava, it is found that a transgenic line is short and small in plant, long and narrow in leaf and more tolerant to low-temperature stress and drought stress, the survival rate is greatly increased, the content of proline in the transgenic line is remarkably increased, the content of malondialdehyde is remarkably reduced after drought or cold stress treatment, and it is shown that the LncRNA and the gene thereof can be used for regulating plant growth and the low-temperature and drought tolerance of plants is improved. In the future, the LncRNA and the gene thereof can be used for carrying out genetic improvement on the plants, and effective resources are provided for promoting plant growth and improving the low-temperature tolerance and drought tolerance of the plants.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an LncRNA and its application. Background technique [0002] Cassava is widely planted in more than 100 countries and regions around the world due to its advantages of drought tolerance, barren tolerance, high yield and no competition for land with major economic crops. Since the introduction of cassava in my country, cassava has been widely planted in tropical and subtropical regions of China. The yield per mu of cassava is high, and cassava root contains a lot of starch, which is one of the main raw materials for the production of starch and industrial alcohol. Although cassava has many advantages, cassava originated in the Amazon River Basin of South America and is a typical tropical crop, so the planting area is limited to the tropical and subtropical regions of our country. Cassava is extremely sensitive to low temperature. Low temperature will reduce the yield of ...

AI Technical Summary

Problems solved by technology

In the past, conventional hybrid breeding methods were used to select cassava varieties resistant to low temperature, but conventional hybrid breeding methods have many limitations: lack of cold-resistant cassava resources, long screening period, high experimental costs, low germination rate of hybrid seeds, and various reasons Caused the difficulty of cross-breeding cassava cold-resistant varieties, with the continuous development of genetic engineering, people began to use methods such as genetic engineering and molecular biology to breed cassava

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