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Application of chitosan oligosaccharide as disease-resistant elicitor to cultivation of plant seedlings

A technology of chitosan oligosaccharides and elicitors, which is applied in the field of application of chitosan oligosaccharides as disease-resistant elicitors in plant seedling cultivation, and can solve problems such as insufficient research, lack of system, and large amounts of data

Inactive Publication Date: 2016-02-24
QINGDAO BZ OLIGO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on callus formation and redifferentiation of oligosaccharides in plant in vitro culture is insufficient, lacking systematic and large data

Method used

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  • Application of chitosan oligosaccharide as disease-resistant elicitor to cultivation of plant seedlings
  • Application of chitosan oligosaccharide as disease-resistant elicitor to cultivation of plant seedlings
  • Application of chitosan oligosaccharide as disease-resistant elicitor to cultivation of plant seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0017] Implementation Case 1: Effect of Oligochitosan on Cucumber Seed Germination

[0018] 1 Materials and methods

[0019] 1.1 Materials and handling

[0020] The tested cucumber seed "Chenfeng 106" was produced by Hainan Chenfeng Seed Industry Co., Ltd. After the seeds are selected, soak them in water with an initial temperature of 45°C for 9 hours, and then take them out. with 0.3% KMnO 4 The solution was sterilized for 30 minutes, rinsed with sterile water for 3 times, and evenly arranged in petri dishes for germination test.

[0021] 1.2 Germination test

[0022] In the experiment, distilled water was used as the control group (CK), and the experimental group was treated with chitosan oligosaccharide solutions with different gradient concentrations (0, 50ppm, 100ppm, 150ppm, 200ppm). Take a dry petri dish with a diameter of 120 mm, and use double-layer filter paper as a germination bed in the petri dish. Arrange the seeds neatly in the petri dish, place 100 imbibed...

Embodiment example 2

[0076] Implementation Case 2: Effect of Oligochitosan on Tomato Callus

[0077] 1 Materials and methods

[0078] 1.1 Drugs and reagents

[0079] COS (chitooligosaccharide), IAA (indole acetic acid), ZT (zeatin), MS medium, agar powder, sucrose, absolute ethanol, sodium hypochlorite.

[0080] 1.2 Materials

[0081] KT3003 tomato seeds

[0082] 1.3 Experimental scheme

[0083] 1.3.1 Acquisition of bacterin and callus

[0084] Select fresh plump KT3003 tomato seeds of the same size and soak them in clean water for preparation, sterilize the seeds with 70% ethanol for 30 seconds, and then rinse them with sterile water for 3 times. Sterilized with 3% sodium hypochlorite for 5 minutes, and finally rinsed with sterile water for 3 times, and the sterilized seeds were inoculated on MS medium.

[0085] When the seeds germinate and grow young cotyledons, randomly select healthy tomato seedlings, cut tomato cotyledons and inoculate them with IAA 1.00mg / L and 0, 0.5mg / L, 1mg / L, 2mg / L...

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Abstract

The invention relates to functional oligosaccharide having the growth promoting function of disease-resistant elicitor in cultivation of plant seedlings, in particular to application of chitosan oligosaccharide as the disease-resistant elicitor to cultivation of the plant seedlings. Chitosan oligosaccharide has the function of promoting seed germination, can enhance quality of the seedlings and promotes formation of strong seedlings. By measuring the content of malondialdehyde related to disease resistance, it is proved that chitosan oligosaccharide is an excellent disease-resistant inducing factor and can be prepared into chitosan oligosaccharide biopesticide to be used. Chitosan oligosaccharide serving as the main component can be combined with other components to be prepared into non-polluted pesticide, and further chitosan oligosaccharide derivative products are developed.

Description

technical field [0001] The invention relates to a functional oligosaccharide with growth-promoting and disease-resistant elicitor functions in plant seedling cultivation, in particular to the application of chitosan oligosaccharide as a disease-resistant elicitor in plant seedling cultivation. Background technique [0002] During the normal growth process, crops are often invaded by various diseases, which affect the yield of crops, and people and animals may cause poisoning after eating diseased crops. In addition, some excellent varieties are often eliminated due to serious diseases and death, leading to the extinction of some rare and rare varieties. By pre-applying elicitors to plants to induce a defense response in the plant, making it in a sensitized state, it can produce systemic resistance to subsequent pathogen infection, thereby enabling the plant itself to have disease resistance and resist diseases. Using this internal resistance mechanism to control diseases wi...

Claims

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Application Information

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IPC IPC(8): A01C1/00A01H4/00A01N43/16A01P21/00
CPCA01C1/00A01H4/001A01N43/16
Inventor 徐小龙张斌管昶王丽丽徐宏伟
Owner QINGDAO BZ OLIGO BIOTECH
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