Wild wheat blue grain character major gene as well as molecular marker and application thereof

A major gene and molecular marker technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problem of unclear major gene of wild single wheat blue grain, and achieve the effect of increasing the content

Active Publication Date: 2021-10-26
SICHUAN AGRI UNIV
View PDF1 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the major gene controlling the blue...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Wild wheat blue grain character major gene as well as molecular marker and application thereof
  • Wild wheat blue grain character major gene as well as molecular marker and application thereof
  • Wild wheat blue grain character major gene as well as molecular marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Transcription group sequencing analysis

[0039] Sequencing samples for red grains crocus and blue gemmate wheat - wild one wheat 4A b (4b) Replacement Z18-1244 paste powder layer ( figure 1 .

[0040] 1) Sequencing and sequence assembly

[0041] Transcription group sequencing can efficiently and quickly obtain all transcripts of biological organizations. Clean Data of each sample has reached 5.97 GB, and the q30 base percentage is 93.18% and above. The Clean Reads of each sample with the specified reference genome, respectively, and the ratio of 84.79% to 87.82%, respectively. Based on the comparison result, variable splicing prediction analysis, gene structure optimization analysis, and new genes were discovered, and 105,787 new genes were discovered, 92,072 function comments were obtained. Gene expression quantity analysis is performed based on comparison results. Differential expression genes are identified according to the expression quantity of the gene in d...

Embodiment 2

[0063] Embodiment 2TBMYC4A molecular characteristics

[0064] 1) Preparation for total RNA and CDNA

[0065] Total RNA was extracted from about 0.5 g of paste powder using the Tiangen Rnaprep Pure Plant Kit (TIANGEN, China). CDNA was obtained from total RNA using the Thermo Revertaid First Strand cDNA kit (Thermo-Fisherscientific, Shanghai).

[0066] 2) Design primer separation TBMYC4A transcription

[0067] TBMYC4ACDS-F: atgcgggaaacagctcag;

[0068] TBMYC4ACDS-R: CTATATAGCTTTCTGAAGCGCTTCA.

[0069] Primers TBMYC4ACDS-F, TBMYC4ACDs-R was used to amplify cDNA to separate wild-granulated wheat grain gene in Z18-1244. PCR amplification was performed using Veriti96 ABI Life 2720 (Hunan Innovagene Biotechnology Limited) using Veriti96 ABI Life 2720.

[0070] The PCR amplification program was 95 ° C degeneration for 5 minutes, 35 cycles: 95 ° C for 30 seconds, 60 ° C for 30 seconds, 72 ° C for 1 minute 40 seconds, and then extension at 72 ° C for 5 minutes. PCR products were purified fr...

Embodiment 3

[0074] Example 3TBMYC4A instantaneous expression can induce anthocyanin synthesis

[0075]The instantaneous expression vector PLGY-02: TBMYC4A was constructed using homologous recombination. The use of TBMYC4F2 and TBMYC4R2 from the TBMYC4A connected to the base sequence of the PGEM-TEASY vector, and the purpose fragment was carried out by high quality kits, and the electrophoresis was detected without miscellaneous tape. The PLGY-02 linearized carrier is mixed with the TBMYC4A recovery fragment 1: 2 concentration, and the link 37 ° C by the CloneXpress II One Step Cloning Kit kit is 30 min after 30 min, it is placed on ice, and E. coli DH5α is converted to E. coli DH5α, overnight culture, Picking the single spot on the recombinant reaction conversion plate for PCR colony detection, positive single spots sent to Shanghai's generic organism for sequencing. pBRACT214: TaMYC1 and pBRACT214: TaMYB7D interaction has been validated as a white coleoptile capable of inducing a large numbe...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a wild wheat blue grain character major gene as well as a molecular marker and application thereof. The gene is a wild wheat grain gene identified in a common wheat-wild wheat grain 4Ab (4B) substitution line, the gene is a major gene for regulating and controlling the wild wheat blue grain character, and anthocyanin synthesis can be regulated and controlled. The nucleotide sequence of the wild wheat blue grain major gene is shown as SEQ ID NO.1, and the amino acid sequence of the encoded protein of the major gene is shown as SEQ ID NO.2. The invention provides a basis for researching an anthocyanin synthesis mechanism of the wild wheat, and also provides a direction and a target spot for improving the content of anthocyanin by transforming plants through a genetic engineering means.

Description

Technical field [0001] The present invention belongs to the field of plant gene engineering, and in particular, the present invention relates to a wild-granulated wheat blue-grain-seminal primary primary gene and molecular markers and applications thereof. Background technique [0002] Anthocyanin has been proven to be effective for human diet and health, because they have anti-inflammatory, antioxidant and anti-cancer properties and improve vision, and found a group of anthocyanins in the paste of blue wheat. pigment. 22 different anthocyanins have been identified in the blue wheat line. Various berries, purple peel wheat, vegetables, red rice and black rice and corn cobs are cornflower -3-glucosides. However, the main anthocyanins in the blue wheat are flying birdhetin-3-glucosides. Flying birdhetin-3-glucoside is the most effective angiogenesis inhibitor in anthocyanins, indicating that it may have a good role in cancer prevention and treatment. [0003] Since the blue cyanin ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/29C12N15/82C12N15/11C07K14/415A01H5/00A01H6/46C12Q1/6895
CPCC07K14/415C12N15/825C12Q1/6895C12Q2600/13
Inventor 张连全柳欣张明虎刘宝龙郝明姜博黄林甯顺腙袁中伟刘登才
Owner SICHUAN AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap