Anthocyanin biosynthesis in carrot plants

A carrot and plant technology, applied in angiosperms/flowering plants, microorganisms, plant peptides, etc., can solve problems such as unclear regulatory genes
CN114096673APending Publication Date: 2022-02-25OTERRA AS +1
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
OTERRA AS
Publication Date
2022-02-25

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Abstract

The present invention provides transgenic carrot plants comprising at least one heterologous DNA sequence encoding: (a) a promoter of a transcription factor gene; or (b) a transcription factor geneoperably linked to a promoter; wherein the heterologous DNA sequence increases the expression of at least one gene encoding a sinapic acid glucosyltransferase (USAGT). The transcription factor gene is preferably DcMYB90 (SEQ ID NO:1) or DcEGL1 (SEQ ID NO:2) or a sequence having at least 80% identity to the sequence of SEQ ID NO:1 or at least 80% identity to the sequence of SEQ ID NO:2, wherein the sequence encodes a transcription factor protein increasing the expression of sinapic acid glucosyltransferase (USAGT) having the sequence of SEQ ID NO:4.
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Description

technical field

[0001] The present invention relates to transgenic carrot plants comprising at least one gene encoding a transcription factor or a heterologous DNA sequence encoding a transcription factor gene operably linked to a promoter, which is expressed in the transgenic plant and increases at least one gene encoding a mustard Expression of acid glucosyltransferase (USAGT) gene. The sequence of USAGT can be SEQ ID NO:4, or a sequence at least 85% identical to SEQ ID NO:4. The present invention also relates to the DNA sequences encoding the transcription factor genes DcMYB90 (SEQ ID NO: 1) and DcEGL1 (SEQ ID NO: 2), and transgenic carrot plants comprising the corresponding DNA sequences operably linked to heterologous promoters. The transgenic plants of the present invention may be used in a method of preparing an anthocyanin (anthocyanin)-containing composition, which method may comprise producing a transgenic carrot plant of the present invention and isolating the anth...

Claims

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