Protein capable of regulating and controlling content of anthocyanin in blueberry fruit as well as encoding gene and application of protein
An anthocyanin content, fruit technology, applied in application, genetic engineering, plant genetic improvement and other directions, can solve problems such as less obvious effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0072] Embodiment 1, the acquisition of transcription factor VcMYB5 and its coding base
[0073] The total RNA of the blueberry variety 'Sierra' was extracted and reverse-transcribed to obtain cDNA, and then PCR amplification was performed using primer F and primer R using the obtained cDNA as a template.
[0074] Primer F: 5'-ATGAATTTATCTGAGACCAG-3';
[0075] Primer R: 5'-TCAAATCAACAATGATTCGG-3'.
[0076] The amplified product is sequenced, and it can be seen that the obtained cDNA nucleotide sequence is sequence 2 in the sequence table, compared with the sequence in Genbank, it is determined that it belongs to the blueberry R2R3-MYB class transcription factor, and the gene shown in it is named VcMYB5, The encoded protein is named VcMYB5, and its amino acid sequence is sequence 1 in the sequence listing.
[0077] The above cDNA (Sequence 1) can also be obtained by artificial synthesis.
Embodiment 2
[0078] Example 2. Establishment of a gene transient VIGS silencing system in blueberry fruit and a functional study of the VcMYB5 gene
[0079] 1. Acquisition of recombinant expression vectors
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com