Protein capable of regulating and controlling content of anthocyanin in blueberry fruit as well as encoding gene and application of protein

An anthocyanin content, fruit technology, applied in application, genetic engineering, plant genetic improvement and other directions, can solve problems such as less obvious effect

Active Publication Date: 2017-07-21
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Early studies on plant anthocyanin biosynthesis mainly focused on the related genes in the anthocyanin biosynthetic pathway, and the content of plant anthocyanin was increased by heterolo...

Method used

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  • Protein capable of regulating and controlling content of anthocyanin in blueberry fruit as well as encoding gene and application of protein
  • Protein capable of regulating and controlling content of anthocyanin in blueberry fruit as well as encoding gene and application of protein
  • Protein capable of regulating and controlling content of anthocyanin in blueberry fruit as well as encoding gene and application of protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Embodiment 1, the acquisition of transcription factor VcMYB5 and its coding base

[0073] The total RNA of the blueberry variety 'Sierra' was extracted and reverse-transcribed to obtain cDNA, and then PCR amplification was performed using primer F and primer R using the obtained cDNA as a template.

[0074] Primer F: 5'-ATGAATTTATCTGAGACCAG-3';

[0075] Primer R: 5'-TCAAATCAACAATGATTCGG-3'.

[0076] The amplified product is sequenced, and it can be seen that the obtained cDNA nucleotide sequence is sequence 2 in the sequence table, compared with the sequence in Genbank, it is determined that it belongs to the blueberry R2R3-MYB class transcription factor, and the gene shown in it is named VcMYB5, The encoded protein is named VcMYB5, and its amino acid sequence is sequence 1 in the sequence listing.

[0077] The above cDNA (Sequence 1) can also be obtained by artificial synthesis.

Embodiment 2

[0078] Example 2. Establishment of a gene transient VIGS silencing system in blueberry fruit and a functional study of the VcMYB5 gene

[0079] 1. Acquisition of recombinant expression vectors

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Abstract

The invention discloses a protein capable of regulating and controlling the content of anthocyanin in a blueberry fruit as well as an encoding gene and application of the protein. The protein provided by the invention is any one of the following proteins: (a) a protein consisting of an amino acid sequence as shown in the sequence 1, (b) a protein which is obtained via performing substitution and/or deletion and/or adding of one or several amino acid residues on the amino acid sequence as shown in the sequence 1, comes from blueberries, is relevant to the content of anthocyanin in a plant product and is derived from the sequence 1, and (c) a protein which is homologous with the amino acid sequence defined by (a) or (b) by 99 percent or above, 95 percent or above, 90 percent or above, 85 percent or above or 80 percent or above, comes from the blueberries and is relevant to the content of the anthocyanin in the plant product. The protein and the encoding gene thereof which are provided by the invention are used for regulating and controlling biological synthesis of the anthocyanin in the blueberry fruit. The protein has important significance for cultivation of a new blueberry variety with higher anthocyanin content.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering, and relates to a protein for regulating anthocyanin content in blueberry fruit, its coding gene and application. Background technique [0002] Blueberry is a plant of the genus Vaccinium in the Ericaceae family. The fruit is rich in anthocyanins, which have health functions such as preventing brain aging, strengthening the heart, anti-cancer, softening blood vessels, and enhancing human immunity. Blueberries can be eaten fresh, and can also be processed into fruit juice drinks, fruit wine drinks, etc., which have extremely high economic value. With the improvement of human health awareness, more and more people show their favor for foods rich in anthocyanins. Therefore, the demand for blueberries in the world, especially in my country, is increasing year by year, and the quality requirements of consumers for blueberries are also increasing. Higher and higher. Therefore, it is of great th...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00
CPCC07K14/415C12N15/8205C12N15/8218C12N15/825C12N15/8261
Inventor 张凌云刘中帅
Owner BEIJING FORESTRY UNIVERSITY
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