Recombinant vector for rapidly obtaining non-GMO genome editing plants and method for using same

Abstract

Provided are a recombinant vector for rapidly obtaining non-GMO genome editing plants and a method for using the same. The recombinant vector comprises an expression element containing PAP1 and NtFT;The original vector is a vector of CRISPR / Cas9 for plant gene editing containing sgRNA gene, Cas9 gene and screening tag gene; The NtFT expression element produces proteins that promote early flowering and the PAP1 expression element produces proteins that promote anthocyanin biosynthesis in plants. The invention can greatly shorten the time for obtaining the gene editing mutation offspring, and screens the offspring plants by plant color, thus not only retaining the offspring of the target gene mutation, but also ensuring that the mutation offspring does not contain the transgenic fragment, and simultaneously can significantly improve the gene editing efficiency. The invention can be used for preparing the gene editing mutation offspring, and can greatly shorten the time for obtaining thegene editing mutation offspring. The method of the invention is simple, fast, cheap and efficient.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a recombinant vector for obtaining non-transgenic gene editing plants and a method for using it. Background technique [0002] In 2012, clustered regularly interspaced short palindromic repeats (Clustered regularly interspaced short palindromic repeats / CRISPR-associated protein 9, CRISPR-associated protein 9, CRISPR / Cas9) and related nuclease Cas9 were reported to be used for gene editing. CRISPR / Cas9 is a new gene editing system developed after Zinc finger nuclease (ZFN) and transcription activator like effector nuclease (TALEN). After just a few years of development, it has quickly become the most successful and widely used genome editing technology because of its simple operation, high editing efficiency, and low off-target efficiency. The CRISPR / Cas9 system has been successfully applied to major food crops such as rice, wheat, corn, soybeans, and economic crops such as cotton, t...

AI Technical Summary

Problems solved by technology

For the screening of non-GMO ingredients in the T1 generation, various derivative methods based on PCR technology are generally used, but DNA extraction, specific primer PCR amplification and electrophoresis detection are basically required, which is time-consuming, laborious, expensive and prone to false negative results

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