86 results about "Amicyanin" patented technology

A method for determining whether a microorganism produces an AmpC β-lactamase is disclosed in which a culture of a microorganism suspected of producing a β-lactamase that inactivates a β-lactam-containing antibiotic is admixed with an effective amount of each of i) a β-lactam-containing antibiotic, ii) a β-lactamase inhibitor to which AmpC β-lactamase is resistant, and iii) a permeabilizing agent for the microorganism present in a non-growth-inhibiting microorganism-permeabilizing amount to form an assay culture. That assay culture in maintained under appropriate culture conditions and for a time period sufficient to determine the interaction of the microorganism with the AmpC β-lactamase resistant inhibitor and antibacterial compound, and thereby determine the presence of an AmpC β-lactamase, wherein a positive test indicates the presence of an AmpC β-lactamase.

Methods, pharmaceutical, dietary supplement, and nutraceutical compositions, and compounds for reducing body weight, modulating body lipid metabolism, and reducing food intake in mammals are provided. The compounds of the invention include fatty acid ethanolamide compounds, homologues and analogs of which the prototype is the endogenous fatty acid ethanolamide, oleoylethanolamide.

A method for promoting the colouring on fruit (apple, pear, peach, grape, etc) features that the 5-aminolevulinic acid (ALA), or levulinic acid (LA)1 or glutamic acid (Glu) is applied to the fruit.

The invention discloses a preparation method of silk fibre with a function of removing formaldehyde, mainly comprising the following steps: (1) dissolving procyanidin with the weight equivalent to 8-12 percent of the silk fibre into water, adding 0.2-0.5 milliliters of glacial acetic acid per each litre of water into the solution and uniformly stirring, adding ferrous sulphate into the solution according to the mass ratio of the ferrous sulphate to the procyanidin of 1 to 1-3 and stirring, adding a sodium bicarbonate solution into the solution to regulate the pH value of the mixing solution to 5-8, increasing the temperature of the mixing solution to 50-60 DEG C, and preserving the heat of the mixing solution for 20-40 minutes; (2) adding 0.5 gram of peregal O and 2-10 grams of salt intoeach litre of the obtained mixing solution and uniformly stirring; (3) adding the silk fibre into the obtained mixing solution and processing for 1-12 hours under the conditions that temperature is 25-90 DEG C and liquor ratio is 1 to 20-40, and then taking the silk fibre out and washing and drying the silk fibre. The obtained silk fibre can enable the removal rate of the formaldehyde contained inindoor air to achieve more than 75 percent.

A cytotoxin can be rendered non-toxic by charge neutralizing the amino acids salient to pore assembly and/or sterically inhibiting formation of the peptide's active conformation. In the presence of specific proteases, the inactive peptide or procytotoxin can be activated to assemble into its lytic conformation and selectively destroy a target cell.

The invention discloses a Brassica napus, parent species Chinese cabbage, cabbage MYBL2 (v-myb avian myeloblastosis viral oncogene homolog-like 2) gene family. The Chinese cabbage MYBL2 gene family comprises a BrMYBL2-1 gene and a BrMYBL2-2 gene. The cabbage MYBL2 gene family comprises a BoMYBL2-1 gene and a BoMYBL2-2 gene. The Brassica napus MYBL2 gene family comprises a BnMYBL2-1 gene, a BnMYBL2-2 gene, a BnMYBL2-3 gene, a BnMYBL2-4 gene. Eight MYBL2 genes have higher homology. The invention also discloses the application of the above gene families in molecular breeding with characters, such as plant seed coat color, seed coat thickness and the like. After a just overexpression plant carrier is built and double 10 number in the black Brassica napus variety, the Brassica napus is compared with the contrast of the explant of a non-transgenosis, transgenosis plants normally grow and develop, pigments such as Proanthocyanidins in the seed coat are reduced, and meanwhile the seed coat becomes thin to form the transgenosis yellow seed character.

The invention relates to the field of molecular breeding, and in particular to an insertion/deletion (InDel) molecular marker AAD closely-linked to the tomato anthocyanin absent character and application thereof. The physical position of the InDel molecular marker closely-linked to the tomato anthocyanin absent character is No. 45339693 base to No. 45343097 base on chromosome 2 of tomato. The invention can replace a traditional method of selecting a green plant to ensure that a breeding material of tomato contains an anthocyanin absence (AA) mutation site. An AA site hybrid plant in the breeding material with normal phenotype is assist-selected by mean of the molecular marker, the breeding period can be shortened, and the breeding efficiency can be improved.

The invention discloses a CAPS (cleaved amplified polymorphic sequence) molecular marking method for identifying solanum lycopersicum with purple black striped pericarp and an application. The sequence of a molecular marker carried by the solanum lycopersicum with purple black striped pericarp is a nucleotide base sequence shown as SEQ ID NO.1, PCR amplification is performed with genomic DNA of the solanum lycopersicum with purple black striped pericarp as a template, nucleotide base sequences shown as SEQ ID NO.3-4 are amplification primers, amplification and cleavage are performed, a specific band different from other solanum lycopersicum pericarp colors can be obtained after cleavage, and the molecular marking method is applied to breed improvement and breeding of the solanum lycopersicum. Compared with the prior art, the CAPS molecular marking method has the advantages that the polymorphism of a cleaved fragment is observed through PCR amplification, cleavage and gel electrophoresis separation, the step of membrane transfer in the traditional RFLP (restricted fragment length polymorphism) analysis is omitted, the operation is simplified, the precision of the RFLP technology is kept, the polymorphism detecting probability is higher, the solanum lycopersicum with purple black striped pericarp is obtained rapidly and applied to breed improvement and assisted breeding, and the method has important theoretical and practical meanings for culturing new varieties of solanum lycopersicum containing rich anthocyanidin.

A composition having: a proanthocyanidin; and a macromolecule, an assembly of macromolecules, a semi-solid, or a solid surface to which the proanthocyanidini is immobilized.

The invention discloses a gene PpRd for regulating fruit flesh cyanin synthesis and application thereof, relating to plant gene engineering and biotechnology. The gene PpRd nucleic acid sequence is disclosed as SEQ ID NO.1, and the protein sequence coded by the gene PpRd is disclosed as SEQ ID NO.2. The application of the PpRd gene is implemented by (1) designing a molecular marker WPS23 according to the sequence of the PpRd gene, and applying the molecular marker WPS23 in marker auxiliary screening in breeding; and (2) application of expression protein sequence and gene functions: detecting and screening cyanidin content related phenotype according to the expression level of the gene, or enhancing or lowering the gene expression level by genetic transformation to obtain the expected flesh color character. The invention obtains a key gene for cyanin accumulation. When the PpRd gene is used for marker auxiliary selection to instruct hybridization breeding of peaches, the screening can be performed in advance, thereby saving the breeding time and enhancing the breeding efficiency.

The invention relates to a therapy of an infection with a drug resistant bacterium wherein a characteristic of multivalent phenol derivatives and/or extracts from “Tara” which enhance the activity of β-lactam antibiotics on the drug resistant bacteria is utilized. Specifically, the invention relates to an enhancer of β-lactam antibiotics comprising a multivalent phenol derivative or its pharmaceutically acceptable salt, a pharmaceutical composition comprising a β-lactam antibiotic and a multivalent phenol derivative and/or extract from “Tara” for the therapy of an infection with a drug resistant bacterium, a disinfectant for the same, and a functional food comprising a multivalent phenol derivative and/or extract from “Tara”.

The present invention relates to broad spectrum β-lactamase inhibitors. More particularly, the invention relates to inhibitors of Class B metallo (MBL) and Class D (OXA) β-lactamases. A method of treating a bacterial infection is provided, wherein the method comprises administering to a mammalian patient in need of such treatment a compound of formula (I)

wherein

R₁ is selected from

R₂ is selected from

with certain provisos as herein defined;

in combination with a pharmaceutically acceptable β-lactam antibiotic in an amount which is effective for treating the bacterial infection.

This invention relates to certain tricyclic 6-alkylidene penems which act as a inhibitor of class-D enzymes. β-Lactamases hydrolyze β-lactam antibiotics, and as such serve as the primary cause of bacterial resistance. The compounds of the present invention when combined with β-lactam antibiotics will provide an effective treatment against life threatening bacterial infections.

In accordance with the present invention there are provided compounds of formula I which are useful for treatment of bacterial infections having class-D enzymes associated therewith:

wherein: One of A and B denotes hydrogen and the other an optionally substituted fused tricyclic heteroaryl group; and X is S or O.

The invention relates to a compound with oxidation resistance and metabolism acceleration functions. The inventive compound is formed by Cordyceps sinensis fungus, cyanin and veralk alcohol. The compound uses the Cordyceps sinensis fungus and grape extractive as main material, with low cost and wide sources. The invention has simple production.

Sterically-awkward 6-β-substituted P-lactam compounds as inhibitors of β-lactamase activity, such compounds as can be used in conjunction with one or more β-lactam antibiotics in a system for treatment of a β-lactam resistant bacterial infection.

Provided are a recombinant vector for rapidly obtaining non-GMO genome editing plants and a method for using the same. The recombinant vector comprises an expression element containing PAP1 and NtFT;The original vector is a vector of CRISPR/Cas9 for plant gene editing containing sgRNA gene, Cas9 gene and screening tag gene; The NtFT expression element produces proteins that promote early flowering and the PAP1 expression element produces proteins that promote anthocyanin biosynthesis in plants. The invention can greatly shorten the time for obtaining the gene editing mutation offspring, and screens the offspring plants by plant color, thus not only retaining the offspring of the target gene mutation, but also ensuring that the mutation offspring does not contain the transgenic fragment, and simultaneously can significantly improve the gene editing efficiency. The invention can be used for preparing the gene editing mutation offspring, and can greatly shorten the time for obtaining thegene editing mutation offspring. The method of the invention is simple, fast, cheap and efficient.

The purpose of the present invention is to provide a process for protection of enzymatic biocatalysts, constituted by a primary support, wrapped by a secondary matrix. More particularly, the invention is embodied as a bioreactor with a configuration that preserves the physical integrity and catalytic activity of the biocatalyst after long runs, and at the same time allows an easy separation of the protected biocatalyst from the solid, precipitated during the reaction course. Still more particularly, the invention refers to the use of the bioreactor herein described for the enzymatic synthesis of β-lactam antibiotics, including the insoluble enzymatic catalysts, which have a primary matrix for immobilization involved by a secondary matrix, which is able to preserve 100% of the catalyst physical integrity and approximately 100% of its catalytic activity.

The invention discloses eggplant flavanone 3-hydroxylase SmF3H as well as a gene and application thereof; nucleotide sequences of cDNA and gDNA of the gene are respectively as shown in SEQ ID NO.1 and SEQ ID NO.2; and the amino sequence coded by the gene is as shown in SEQ ID NO.3. The nucleotide sequence of the promoter sequence of the gene is as shown in SEQ ID NO.4. The gene is expressed in root, stem, leaf, flower, peel and pulp, and the expression amount in the stem is significantly higher than that in other tissues. Under low-temperature stress, the expression amount achieves a maximum value at 12thh, which is 2.34 times as much as that unprocessed. An over-expression vector of the eggplant SmF3H is constructed and is converted into arabidopsis thaliana, discovering that anthocyanin contents in stem and fruit pod are significantly improved. The invention provides theoretical basis for improving plant quality by virtue of gene engineering technology in the future so as to obtain highly anti-oxidative medicines or food; therefore, the eggplant flavanone 3-hydroxylase SmF3H is quite high in application value.

The invention discloses brown cotton fiber procyanidine transfer-related GST protein gene and application thereof. The gene has a nucleotide sequence shown as SEQ ID NO.1 and an amino acid sequence shown as SEQ ID NO.2. According to the invention, the gene is found to be capable of controlling a formation of fiber pigment and improving plant color and luster through gene coded sequence analysis and genetic transformation of Arabidopsis thaliana; thus, reference basis is provided for utilizing a gene engineering technology to genetically improve brown cotton color characters, and a novel path is provided for cultivating a new brown cotton variety with stable fiber pigment.

The invention relates to an analysis method for researching formation of variegation of nelumbo nucifera based on combination of transcriptomics with proteomics TMT, and provides a new method for disclosing a molecular mechanism of the formation of the variegation of plants. Samples of "Dasajin" nelumbo nucifera petals are collected, through transcriptome Illumina HiSeq sequencing, expression difference genes on an anthocyan metabolic pathway are screened, besides, proteins in the samples are extracted, the concentration of the proteins is determined, the quality is detected, through TMT labelmarking and 2D-LC-MS analysis, bioinformatics analysis is performed through combining with the proteomics, the proteins for adjusting and controlling expressing level differences on the anthocyan metabolic pathway are screened, the genes having differences in the transcriptome are related into the proteomics, and key proteins and corresponding genes for adjusting and controlling the variegation of "Dasajin" nelumbo nucifera are screened, so that the purpose of understanding a manner of the proteins and the genes for adjusting the formation of the variegation of the "Dasajin" nelumbo nuciferais achieved, and a new thinking and a new method are provided for the formation mechanism of the variegation of other plants.

The invention discloses a complete sequence of an anthocyanidin reductase gene from Rubus ideaus L. and a preparation method thereof. According to the invention, Rubus ideaus L. is used as a raw material, a method of combined RT-PCR technology and RACE technology is employed, and the full length cDNA of the anthocyanidin reductase gene (RiANR) from Rubus ideaus L. is cloned; the cDNA comprises an open reading frame with a size of 1020 bp and codes a protein composed of 339 amino acids and having a theoretical isoelectric point of 5.81 and a theoretical molecular weight of 36413.8 D, which are identical to the results of prokaryotic expression SDS-PAGE electrophoresis detection. Cloning and expression analysis are carried out on the anthocyanidin reductase RiANR gene from Rubus ideaus L. in the invention, so a base material is provided for further exploration of the regulation and control mechanism of proanthocyanidin from Rubus ideaus L., and important gene resources are provided for acquiring novel crop varieties with high proanthocyanidin content through genetic improvement.