CAPS (cleaved amplified polymorphic sequence) molecular marking method for identifying solanum lycopersicum with purple black striped pericarp and application

Abstract

The invention discloses a CAPS (cleaved amplified polymorphic sequence) molecular marking method for identifying solanum lycopersicum with purple black striped pericarp and an application. The sequence of a molecular marker carried by the solanum lycopersicum with purple black striped pericarp is a nucleotide base sequence shown as SEQ ID NO.1, PCR amplification is performed with genomic DNA of the solanum lycopersicum with purple black striped pericarp as a template, nucleotide base sequences shown as SEQ ID NO.3-4 are amplification primers, amplification and cleavage are performed, a specific band different from other solanum lycopersicum pericarp colors can be obtained after cleavage, and the molecular marking method is applied to breed improvement and breeding of the solanum lycopersicum. Compared with the prior art, the CAPS molecular marking method has the advantages that the polymorphism of a cleaved fragment is observed through PCR amplification, cleavage and gel electrophoresis separation, the step of membrane transfer in the traditional RFLP (restricted fragment length polymorphism) analysis is omitted, the operation is simplified, the precision of the RFLP technology is kept, the polymorphism detecting probability is higher, the solanum lycopersicum with purple black striped pericarp is obtained rapidly and applied to breed improvement and assisted breeding, and the method has important theoretical and practical meanings for culturing new varieties of solanum lycopersicum containing rich anthocyanidin.

Description

technical field [0001] The invention belongs to the field of biological detection, in particular to a CAPS molecular marker method and application for identifying purple-black striped peel tomatoes. Background technique [0002] Tomato (Solanum lycopersicum) belongs to Solanaceae Solanum and is the largest vegetable crop in the world. It originated from the Andes in South America and was introduced to Europe by Spanish colonists around the 16th century. After several intense domestication and improvements, it became the modern cultivated tomato. Compared with other traits, the domestication process of tomato pulp color is relatively short, and almost all of them emerged in the last 100 years. The ripe fruit color of existing cultivated tomato is mainly red, yellow and pink, and this is because the reason that is rich in pigments such as lycopene and carotenoid in the fruit. Although lycopene and carotenoids are natural pigments that are very beneficial to the human body, th...

AI Technical Summary

Problems solved by technology

Although lycopene and carotenoids are natural pigments that are very beneficial to the human body, these two types of pigments are fat-soluble substances that must be properly cooked or processed to be well absorbed by the human body. role is more difficult

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