Method for obtaining plant hairy roots with high anthocyanin content
A technology with high anthocyanins and hairy roots, applied in the field of plants, can solve problems such as difficulty in construction work, difficulty in obtaining hairy roots, and sexual hybridization of hairy roots
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[0014] This specific embodiment adopts following technical scheme: the steps that it obtains are as follows:
[0015] (1), design primers comprising AttB1adapter and AttB2adapter, connect the MYB genes AmROS1 and AmDEL that regulate anthocyanin synthesis and metabolism in plants to the vector pDONR207 through the BP reaction of Gateway cloning; then use the LR reaction of Gateway cloning to separate AmROS1 and AmDEL Ligated into vector pJAM1502 (kanamycin resistance) and vector pK7WG2D (spectinomycin resistance) to obtain pJAM1502::AmROS1 and pK7WG2D::AmDEL;
[0016] (2), using the method of electroporation to transform PJAM1502::AmROS1 and pK7WG2D::AmDEL into Agrobacterium rhizogenes (rifampicin resistance), and select by rifampicin, kanamycin and spectinomycin antibiotics, Agrobacterium rhizogenes to obtain pJAM1502::AmROS1 and pK7WG2D::AmDEL;
[0017] (3), tobacco germinates and grows on the aseptic medium for 19 days, cuts the tobacco leaves into small squares in an ultra...
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