Ornamental collard anthocyanin relevant R2R3-MYB gene BoMYB and expression vector and application thereof

A technology of R2R3-MYB and kale, applied in the field of genetic engineering

Inactive Publication Date: 2018-05-04
QIQIHAR UNIVERSITY
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Problems solved by technology

So far, the research on the synthesis of kale anthocyanins is still in the preliminary exploration stage (Zhang Bin, 2014), and the gene cloning and regulation mechanism of transcription factors have not been reported yet.

Method used

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  • Ornamental collard anthocyanin relevant R2R3-MYB gene BoMYB and expression vector and application thereof
  • Ornamental collard anthocyanin relevant R2R3-MYB gene BoMYB and expression vector and application thereof
  • Ornamental collard anthocyanin relevant R2R3-MYB gene BoMYB and expression vector and application thereof

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Embodiment Construction

[0025] The technical solution of the present invention will be further described below in conjunction with the accompanying drawings, but it is not limited thereto. Any modification or equivalent replacement of the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention should be covered by the present invention. within the scope of protection.

[0026] The present invention intends to clone the R2R3-MYB protein gene related to anthocyanin regulation from purple-leaved kale, analyze its expression characteristics in different organs, use Agrobacterium-mediated transformation of Arabidopsis thaliana, and explore the effect of heterologous expression of BoMYB on transgenes. The effect of phenotypic changes in Arabidopsis, further verification and discussion of the gene function and the relationship with anthocyanins. Specific steps are as follows:

[0027] 1 Materials and methods

[0028] 1.1 Ma...

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Abstract

The invention discloses an ornamental collard anthocyanin relevant R2R3-MYB gene BoMYB and an expression vector and application thereof. The cDNA sequence of the BoMYB gene is shown as SEQ ID NO.1. The BoMYB gene can be used for regulating and controlling the biosynthesis of the anthocyanin. The collard anthocyanin purple leaf property regulating and control gene BoMYB is obtained by a homologouscloning method; a real-time fluorescent quantitative PCR method is used for studying the expression mode of the BoMYB gene in different tissues; analysis results show that the tissue specificity is realized in the BoMYB expression; the gene expression quantity is relevant to the anthocyanin content in each tissue. The heterologous expression verification in arabidopsis proves that the accumulationof the anthocyanin in the transgenic arabidopsis plants can be increased through the BoMYB overexpression.

Description

[0001] This study was supported by the National Natural Science Foundation of China (No.31401908); the Natural Science Foundation of Heilongjiang Province (No.C2016056); the Youth Innovative Talents Training Program of Heilongjiang General Universities (No.UNTYSCT-2017155) and the young academic backbone of the Heilongjiang Provincial Department of Education Co-financed by project (No. 135209313). technical field [0002] The invention belongs to the technical field of genetic engineering and relates to an ornamental kale anthocyanin MYB gene and its expression vector and application. Background technique [0003] Kale is native to the Mediterranean and Asia Minor. It is a 2-year-old herbaceous plant. It is a variety of Brassica Brassica species in the Brassicaceae family. It is the same as wild cabbage, head cabbage, purple cabbage, savoy cabbage, kohlrabi and Brussels sprouts. Belonging to the coastal area of ​​Northwest Europe, it can be used for urban greening, and it is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82A01H5/00A01H6/20
CPCC07K14/415C12N15/825
Inventor 王玉书王欢高美玲张志
Owner QIQIHAR UNIVERSITY
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