Eggplant flavanone 3-hydroxylase SmF3H as well as gene and application thereof

A technology of flavanone and hydroxylase, which is applied in the fields of application, genetic engineering, oxidoreductase, etc., can solve the problems of unexamined and lagging eggplant anthocyanin research, and achieve the effect of great application value
CN105087508AInactive Publication Date: 2015-11-25SHANGHAI JIAO TONG UNIV
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
SHANGHAI JIAO TONG UNIV
Publication Date
2015-11-25
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

The invention discloses eggplant flavanone 3-hydroxylase SmF3H as well as a gene and application thereof; nucleotide sequences of cDNA and gDNA of the gene are respectively as shown in SEQ ID NO.1 and SEQ ID NO.2; and the amino sequence coded by the gene is as shown in SEQ ID NO.3. The nucleotide sequence of the promoter sequence of the gene is as shown in SEQ ID NO.4. The gene is expressed in root, stem, leaf, flower, peel and pulp, and the expression amount in the stem is significantly higher than that in other tissues. Under low-temperature stress, the expression amount achieves a maximum value at 12thh, which is 2.34 times as much as that unprocessed. An over-expression vector of the eggplant SmF3H is constructed and is converted into arabidopsis thaliana, discovering that anthocyanin contents in stem and fruit pod are significantly improved. The invention provides theoretical basis for improving plant quality by virtue of gene engineering technology in the future so as to obtain highly anti-oxidative medicines or food; therefore, the eggplant flavanone 3-hydroxylase SmF3H is quite high in application value.
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Description

technical field

[0001] The invention relates to key enzymes and coding genes in the eggplant anthocyanin synthesis pathway, in particular to an eggplant flavanone 3-hydroxylase gene SmF3H, which belongs to the field of biotechnology. Background technique

[0002] The human body will inevitably produce a large number of free radicals due to metabolic activities. As a strong reducing substance, anthocyanins can remove free radicals in the human body and delay the aging of the body. The synthetic pathway of anthocyanins is a part of the synthetic pathway of flavonoids, and it is also a very extensive and in-depth plant secondary metabolic pathway studied at present, which has been elaborated in the main model plants such as Arabidopsis and petunia. The biosynthesis of anthocyanins is mainly divided into three stages: the first stage is from phenylalanine to coumaryl-CoA, which is a common step in many secondary metabolisms; the second stage is from coumaryl-CoA to dihydroflavon...

Claims

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