Brassica napus, parent species Chinese cabbage, cabbage MYBL2 (v-myb avian myeloblastosis viral oncogene homolog-like 2) gene family and application thereof

A technology of Brassica napus, a gene family, applied in the field of genetic engineering, to achieve the effect of reducing the pigment of the seed coat and thinning the seed coat

Inactive Publication Date: 2011-10-26
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, at present, the number of members, protein characteristics, evolutionary relationship, tissue specificity of expression, relationship with yellow seed

Method used

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  • Brassica napus, parent species Chinese cabbage, cabbage MYBL2 (v-myb avian myeloblastosis viral oncogene homolog-like 2) gene family and application thereof
  • Brassica napus, parent species Chinese cabbage, cabbage MYBL2 (v-myb avian myeloblastosis viral oncogene homolog-like 2) gene family and application thereof
  • Brassica napus, parent species Chinese cabbage, cabbage MYBL2 (v-myb avian myeloblastosis viral oncogene homolog-like 2) gene family and application thereof

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Embodiment Construction

[0035] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. The experimental method that does not indicate specific conditions in the preferred embodiment is usually according to conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang, etc., Science Press, 2002) conditions, or as recommended by the manufacturer.

[0036] Plant materials used in the preferred embodiment: Brassica napus is typical black seed line 5B and yellow seed line 09L587, Chinese cabbage is black seed line 09L597 and yellow seed line 09L600 of Brassica napus subspecies (B.rapa ssp.oleifera), cabbage Black seed line 09L598 and yellow seed line 09L599, which are kale varieties (B. oleracea var. acephala), were provided by Chongqing Rapeseed Engineering Technology Research Center and planted under conventional field test conditions. The s...

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Abstract

The invention discloses a Brassica napus, parent species Chinese cabbage, cabbage MYBL2 (v-myb avian myeloblastosis viral oncogene homolog-like 2) gene family. The Chinese cabbage MYBL2 gene family comprises a BrMYBL2-1 gene and a BrMYBL2-2 gene. The cabbage MYBL2 gene family comprises a BoMYBL2-1 gene and a BoMYBL2-2 gene. The Brassica napus MYBL2 gene family comprises a BnMYBL2-1 gene, a BnMYBL2-2 gene, a BnMYBL2-3 gene, a BnMYBL2-4 gene. Eight MYBL2 genes have higher homology. The invention also discloses the application of the above gene families in molecular breeding with characters, such as plant seed coat color, seed coat thickness and the like. After a just overexpression plant carrier is built and double 10 number in the black Brassica napus variety, the Brassica napus is compared with the contrast of the explant of a non-transgenosis, transgenosis plants normally grow and develop, pigments such as Proanthocyanidins in the seed coat are reduced, and meanwhile the seed coat becomes thin to form the transgenosis yellow seed character.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to the MYBL2 gene family of Brassica napus and its parent species Chinese cabbage (Brassica rapa) and cabbage (Brassica oleracea) and applications thereof. Background technique [0002] Brassica (Brassica) is the most important genus of Brassicaceae (Brassicacease), containing worldwide important vegetables, oilseeds and ornamental crops, such as Chinese cabbage (B.rapa), cabbage (B.oleracea) and Brassica napus ( B. napus), wherein Brassica napus is an allotetraploid formed by natural interspecific hybridization and doubling of Chinese cabbage and Brassica napus. Arabidopsis thaliana is the most well-studied model plant from the Brassicaceae. Chromosomal molecular marker collinearity studies found that there were genome-level and gene-level conservation among Brassica plants and between Brassica and Arabidopsis thaliana. [0003] Brassica napus yellow-seed strain has t...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/63C12N15/82C12N5/10A01H5/00
Inventor 柴友荣黎咏蜀陆俊杏李加纳曹廷李波付春杨璨
Owner SOUTHWEST UNIVERSITY
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