Chinese cabbage TT2 gene family and application thereof

A gene family, cabbage technology, applied in the field of genetic engineering, to achieve the effects of reducing seed coat pigment, good application prospects, and restoring the accumulation of seed coat pigment

Inactive Publication Date: 2012-07-11
SOUTHWEST UNIVERSITY
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

However, at present, the number of members, protein characteristics, evolutionary relationship, tissue specificity of expression, relationship with yellow seed t

Method used

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  • Chinese cabbage TT2 gene family and application thereof
  • Chinese cabbage TT2 gene family and application thereof
  • Chinese cabbage TT2 gene family and application thereof

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Embodiment Construction

[0039] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. The experimental method that does not indicate specific conditions in the preferred embodiment is usually according to conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang, etc., Science Press, 2002) conditions, or as recommended by the manufacturer.

[0040] The plant material that preferred embodiment adopts: the material of cabbage is the black seed line 06K130 and the yellow seed line 06K124 of Chinese cabbage type rape subspecies (B.rapa ssp.oleifera), the material of cabbage is kale variety (B.oleracea var.acephala ) black seed line 06K158 and yellow seed line 06K165, the materials of Brassica napus are typical black seed maintainer line 5B, Zhongyou 821, black / yellow seed near-isogenic lines (black seed line L1, yellow seed line L2), all ...

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Abstract

The invention relates to the technical field of gene engineering, in particular to a Chinese cabbage TT2 gene family and application thereof. The gene family of Chinese cabbage transparent seed coat 2 comprises two members such as a BrTT2-1 gene and a BrTT2-2 gene, wherein the two TT2 genes have high homology. The invention further discloses application of the gene family to plant molecular breeding. After expression of the gene family in black seed cabbage type rape is subjected to antisense suppression, transgenic plants are subjected to character change such as color shallowing of seed coat and the like and potential of creating a transgenic yellow seed material is achieved.

Description

[0001] The present invention is a divisional application with the application number 201010281894.8, the application date is September 15, 2010, and the application name is "Brassica napus and its parent species Chinese cabbage and Brassica TT2 gene family and their application". technical field [0002] The invention relates to the technical field of genetic engineering, in particular to a cabbage (Brassica rapa) TT2 (TRANSPARENTTESTA2, transparent seed coat 2; also known as MYB123) gene family and applications thereof. Background technique [0003] Brassica (Brassica) is the most important genus among more than 300 genera in the Brassicaceae (Brassicacease), containing worldwide important vegetables, oilseeds and ornamental crops, such as cabbage (B.rapa), cabbage (B.oleracea) and Brassica napus (B. napus), and Brassica napus is an allotetraploid formed by natural interspecific hybridization and doubling of Chinese cabbage and Brassica napus. Arabidopsis thaliana is the mo...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82A01H5/00
Inventor 柴友荣张迪位运粮杜娟李加纳吕俊唐章林申敏付春
Owner SOUTHWEST UNIVERSITY
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