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Complete sequence of anthocyanidin reductase gene from Rubus ideaus L. and preparation method thereof

A technology of raspberry anthocyanin and raspberry anthocyanin, which is applied in the fields of oxidoreductase, genetic engineering, plant gene improvement, etc., and can solve the problems such as the incomplete clarity of the regulation mechanism of metabolic enzymatic reactions

Inactive Publication Date: 2017-11-03
YUNCHENG UNIVERISTY
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  • Abstract
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  • Claims
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Problems solved by technology

At present, there are few relevant reports on the biosynthesis of raspberry proanthocyanidins, and the regulation mechanism of metabolic enzymatic reactions is not completely clear.

Method used

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  • Complete sequence of anthocyanidin reductase gene from Rubus ideaus L. and preparation method thereof
  • Complete sequence of anthocyanidin reductase gene from Rubus ideaus L. and preparation method thereof
  • Complete sequence of anthocyanidin reductase gene from Rubus ideaus L. and preparation method thereof

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Embodiment Construction

[0035] In order to make the objectives and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention, but not to limit the present invention.

[0036] The plant materials and culture conditions used in the following examples are:

[0037] The tested red raspberry (Rubus idaeus L.) variety is "Qiufu", based on its adult tree young roots, stems, leaves and 7 days after flowering (green fruit period), 14 days after flowering (ginkgo period), flower The fruits of the last 21 days (coloring period, red area>50%<80%)) and 24 days after flowering (maturing period, the fruit surface area is all red) were used as experimental materials, and the raspberry planting demonstration by Yuncheng Yiyuan Modern Agricultural Development Co., Ltd. Conservation of germplasm resources in the garden.

[00...

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Abstract

The invention discloses a complete sequence of an anthocyanidin reductase gene from Rubus ideaus L. and a preparation method thereof. According to the invention, Rubus ideaus L. is used as a raw material, a method of combined RT-PCR technology and RACE technology is employed, and the full length cDNA of the anthocyanidin reductase gene (RiANR) from Rubus ideaus L. is cloned; the cDNA comprises an open reading frame with a size of 1020 bp and codes a protein composed of 339 amino acids and having a theoretical isoelectric point of 5.81 and a theoretical molecular weight of 36413.8 D, which are identical to the results of prokaryotic expression SDS-PAGE electrophoresis detection. Cloning and expression analysis are carried out on the anthocyanidin reductase RiANR gene from Rubus ideaus L. in the invention, so a base material is provided for further exploration of the regulation and control mechanism of proanthocyanidin from Rubus ideaus L., and important gene resources are provided for acquiring novel crop varieties with high proanthocyanidin content through genetic improvement.

Description

Technical field [0001] The invention relates to the field of gene cloning, in particular to a complete sequence of raspberry anthocyanin reductase gene and a production method. Background technique [0002] Raspberry (Rubus ideaus L.) is a berry of the genus Rubus of the Rosaceae family, commonly known as raspberry, tray, and mountain foam. Because its berry is sweet and mellow, rich in nutrients, and rich in ellagic acid (Ellagic acid), anthocyanin (anthocyanin), proanthocyanidin (proanthocyanidin, PA) and other biologically active substances with strong antioxidant properties, it is given the first The three-generation health-care "golden fruit" has the reputation of being a promising fruit tree variety and medicinal plant (Guo Junzhan et al., 2004; Jiang Guihua et al., 2006). [0003] Procyanidins, also known as condensed tannins, belong to flavonoids and are secondary metabolites of a series of reactions in the phenylalanine metabolic pathway (Deluc et al., 2006). Proanthocya...

Claims

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Application Information

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IPC IPC(8): C12N15/53C12N15/10
CPCC12N9/001C12N15/1096C12Y103/01077C12Q2531/113C12Q2549/119
Inventor 刘缙王亚红滕红梅王玉国尉淑珍
Owner YUNCHENG UNIVERISTY
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