Method of extracting procyanidins from unripe fruits of syzygium cumini

A procyanidin, immature technology, applied in the field of extraction and extraction of natural active products, can solve problems such as waste of anthocyanin resources, and achieve the effects of low soluble components, high purity and large amount of procyanidins

Inactive Publication Date: 2014-01-22
JIAYING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the fruit of Hainan rose apple has not been developed and utilized, and a large amount of Hainan rose apple proanthocyanidin resources have been wasted in vain

Method used

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  • Method of extracting procyanidins from unripe fruits of syzygium cumini
  • Method of extracting procyanidins from unripe fruits of syzygium cumini

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Picking of rose apple fruit in Hainan

[0028] From September to October, eight ripe Hainan apple apples with reddish skin or faded green are rinsed 1 or 2 times with deionized water to clean the dust on the surface of the fruit, remove sundries and rotten fruits after fishing, and air-dry for later use .

[0029] 2. Pulverization of Hainan apple fruit

[0030] The dried Hainan apple fruit is crushed by a pulverizer and passed through a 60-mesh sieve, and the particles that cannot pass through the sieve are crushed and sieved again, and the sifted powder is collected as the material for extracting proanthocyanidins.

[0031] 3. Extraction of proanthocyanidins

[0032] Use 70% acetone deionized aqueous solution as the extraction solution of proanthocyanidins for the crushed and sieved material, adjust the pH of the solution to 2 with hydrochloric acid, mix the material and solvent at 1:10 (mass / volume=g / mL), heat to 40-50°C, stirrer at 50 rpm, and stop after stirri...

Embodiment 2

[0044] Rinse the red or faded green roseberry fruit with deionized water for 1 to 2 times, wash off the dust on the surface of the fruit, remove sundries and rotten fruit after fishing, and air-dry; crush the dried roseberry fruit, 140 Sieve through a mesh; use 70% ethanol deionized aqueous solution as the extraction solution of proanthocyanidins, adjust the pH=3 of the solution, and mix the pulverized and sieved Hainan rose fruit material with the solvent at a ratio of 1:10 (mass / volume=gram / ml) , that is, adding 10 ml of solvent to each gram of Hainan rose fruit material) and mixing, heating to 55-65 ° C, stirring at 100 rpm, and stopping after stirring for 120 minutes; filtering, collecting the filtrate, and repeating the extraction of the filter cake once, collecting The filtrate, the extraction rate of proanthocyanidins in the 2 times extraction material is up to 91.11%. The filtrate is distilled under reduced pressure at 60°C and a vacuum of -0.09MPa to recover the alcoho...

Embodiment 3

[0046] Rinse the red or faded green rose apple fruit with deionized water for 1 to 2 times, wash off the dust on the surface of the fruit, remove sundries and rotten fruit, etc., and air-dry; crush the dried rose apple fruit, 10 Sieve through a mesh; use 95% methanol deionized aqueous solution as the extraction solution of proanthocyanidins, adjust the pH=8 of the solution, and mix the pulverized and sieved Hainan rose fruit material with the solvent at a ratio of 1:20 (mass / volume=gram / ml) , that is, add 20 ml of solvent to each gram of Hainan rose fruit material) and mix, heat to 55-65 ° C, stirrer at 100 rpm, and stop after stirring for 360 minutes; filter, collect the filtrate, and repeat the extraction of the filter cake once, collect The filtrate, the extraction rate of proanthocyanidins in the two extraction materials reached 93.11%, and the filtrate was distilled under reduced pressure at 60°C and a vacuum of -0.09MPa to recover the alcohol solvent, so that the alcohol ...

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Abstract

The invention discloses a method of extracting procyanidins from upripe fruits of syzygium cumini, and aims to provide a method of extracting procyanidins, wherein the method has the advantages of simplicity, high extraction rate, low cost and high purity of extracted procyanidins. The technical scheme of the method is characterized by comprising the following steps in sequence: (1) picking up 80 percent ripe fruits of the syzygium cumini, cleaning, drying and crushing the fruits into powder with the granularity of 10-140 meshes; (2) uniformly mixing the powder prepared in the step (1) with a solvent, regulating the pH value, heating to 30-90 DEG C, stirring for 20-360 minutes, performing solid-liquid separation, and collecting a clear solution; (3) distilling the clear solution prepared in the step (2) at reduced pressure to obtain an extracting solution of the procyanidins of the syzygium cumini; and (4) adsorbing the extracting solution of the procyanidins of the syzygium cumini by use of macro-porous adsorption resin, desorbing the solvent, collecting an eluent, distilling and reclaiming the solvent at reduced pressure, and performing spray drying in vacuum to obtain procyanidins powder. The method belongs to the technical field of extraction of natural biological active substances.

Description

technical field [0001] The invention relates to an extraction method, specifically, a method for extracting proanthocyanidins from immature Hainan apple fruit, belonging to the technical field of extraction of natural active products. Background technique [0002] Procyanidins (PC) is a general term for a large class of polyphenolic compounds that widely exist in plants. Until decamers are aggregated. Proanthocyanidins are natural active products that scavenge free radicals and have strong antioxidant properties. For example, the antioxidant capacity of grape seed proanthocyanidins in vivo is 20 times that of Vc and 50 times that of Ve. Proanthocyanidins have various biological activities such as anti-oxidation, anti-tumor, and cardiovascular protection, and are important functional ingredients in health food developed in recent years. However, the source of raw materials for the production of proanthocyanidins is currently limited by seasons and regions, and the supply of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/62
Inventor 张镜
Owner JIAYING UNIV
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