Key glycosyl transferase for piebald formation and coloring as well as coding gene and application thereof

A technology of glycosyltransferase and coding gene, applied in key glycosyltransferase PhUGT78A22 and its coding gene and application field

Pending Publication Date: 2022-06-03
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above studies of the variegated transcriptome were limited to the

Method used

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  • Key glycosyl transferase for piebald formation and coloring as well as coding gene and application thereof
  • Key glycosyl transferase for piebald formation and coloring as well as coding gene and application thereof
  • Key glycosyl transferase for piebald formation and coloring as well as coding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1. Cloning of the gene encoding the key glycosyltransferase PhUGT78A22 in the formation and coloration of mottled

[0039] Plant material:

[0040]The present invention is based on the distant hybrid 'Harmony' (P.'He Xie') (Hao Qing, Liu Zhengan) cultivated in Beijing Botanical Garden (39°59'N, 116°12'E, altitude 70m), Institute of Botany, Chinese Academy of Sciences *, Shu Qingyan, Wang Liangsheng, Chen Fufei. 2008. Discovery and identification of China's first distant hybrid of peony and peony. Journal of Horticulture, 35(6):853-858) as the research object. The petals are divided into spotted and non-spotted parts, which are collected in 4 developmental stages: the first stage of flower buds is not open, and the petals are light yellow green without variegation (level 1); the second stage of flower buds is not opened, and red variegation appears at the base of the petals , the non-spotted part turned from light yellowish green to light pink (grade 2); the thi...

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Abstract

The invention discloses key glycosyltransferase for piebald formation and coloring as well as a coding gene and application of the key glycosyltransferase. The glycosyl transferase provided by the invention is a protein as shown in a) or b): a) a protein consisting of an amino acid sequence as shown in a sequence 1 in a sequence table; and b) a protein which is formed by substitution and/or deletion and/or addition of one or more amino acid residues on the amino acid sequence as shown in the sequence 1 in the sequence table, has mottle formation and coloring glycosyl transferase activity and is derived from a). The glycosyl transferase PhUGT78A22 provided by the invention can be used for accurately catalyzing the conversion of glycosylated anthocyanin in spots or non-spots, so that the difference between petal spots and non-spot anthocyanin in'harmony 'is caused. A new perspective is provided for molecular regulation and control of anthocyanin biosynthesis and accumulation, a new thought is provided for flower color breeding for improving the ornamental value, and reference is provided for deeply knowing a molecular regulation and control mechanism of anthocyanin biosynthesis and accumulation.

Description

technical field [0001] The present invention relates to the field of biotechnology, in particular to PhUGT78A22, a key glycosyltransferase for variegation formation and coloration, and its encoding gene and application. Background technique [0002] Flower coloration, one of nature's most beautiful manifestations, has been associated with evolution and subsequent flower diversification by attracting animal pollinators and participating in responses to biotic and abiotic stresses, which has resulted in the diversity of angiosperms observed today Sex (Mekapogu M, Vasamsetti BMK, Kwon O-K, et al (2020) Anthocyanins in Floral Colors: Biosynthesis and Regulation in Chrysanthemum Flowers. Int J Mol Sci 21:6537. https: / / doi.org / 10.3390 / ijms21186537). The unusual arrangement of colours in flowers relies primarily on four groups of pigments: chlorophyll, carotenoids, flavonoids and betalains (Narbona E, del Valle JC, Whittall JB (2021) Painting the green canvas: how pigments produce ...

Claims

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Application Information

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IPC IPC(8): C12N9/10C12N15/54C12P19/60C12N15/84A01H5/02A01H6/00
CPCC12N9/1051C12P19/60C12N15/825C12N15/827C12N15/8218
Inventor 舒庆艳刘政安李旸
Owner INST OF BOTANY CHINESE ACAD OF SCI
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