Application of purple cabbage anthocyanin biosynthesis regulatory gene BrTT8 to photoinduction type purple tomatoes cultivation and method

A technology for biosynthesis and gene regulation, applied in the biological field, can solve the problems of long cycle and uncertain effect, and achieve the effect of increasing anthocyanin content, good market prospect and economic value

Inactive Publication Date: 2016-04-20
CHONGQING UNIV
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  • Abstract
  • Description
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Problems solved by technology

Traditional breeding methods have a long cycle and uncertain effects

Method used

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  • Application of purple cabbage anthocyanin biosynthesis regulatory gene BrTT8 to photoinduction type purple tomatoes cultivation and method
  • Application of purple cabbage anthocyanin biosynthesis regulatory gene BrTT8 to photoinduction type purple tomatoes cultivation and method
  • Application of purple cabbage anthocyanin biosynthesis regulatory gene BrTT8 to photoinduction type purple tomatoes cultivation and method

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Embodiment Construction

[0025] The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings. For the experimental methods not specified in the examples, the conventional conditions are generally followed, such as the conditions described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al.), or the conditions suggested by the manufacturer.

[0026] The tomato variety used in the embodiment of the present invention is "SolanumlycopersiconMill.cv.AilsaCraig", which is reserved for this laboratory. The pMD19-T vector is a product of Treasure Biological Company, pBI121 vector, Escherichia coli DH5α, Escherichia coli "helper" bacteria (abbreviated as helper) containing the motile plasmid pRK2013 and Agrobacterium tumefaciens LBA4404 are preserved by our laboratory; RNA extraction kit, RNAPCRKit (AMV)Ver.3.0 kit, DNA extraction kit UniversalGenomicDNAExtractionKitVer.3.0, ligation kit DNALigationKitVer.2.0 (T4DNA li...

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Abstract

The invention discloses application of a purple cabbage anthocyanin biosynthesis regulatory gene BrTT8 to photoinduction type purple tomatoes cultivation and a method. The nucleotide sequence of the purple cabbage anthocyanin biosynthesis regulatory gene BrTT8 is shown as SEQ ID NO. 3. The gene is constructed to be a recombinant expression vector, and converted into tomato leaves through an agrobacterium-mediated transformation method, finally transgenic tomato plants are obtained, and when the transgenic tomato plants are irradiated by strong light, stems, leaves, petals and upper ends of fruits of transgenic tomatoes are purple, the content of anthocyanin and the content of flvonol in the fruits are improved remarkably; synthesis of anthocyanin does not exist in tissue of transgenic tomatoes cultivated under weak light irradiation, the risks of diseases such as cancer and cardiovascular can be reduced by eating food rich in ingredients such as anthocyanin and flavone regularly, the obtained transgenic tomatoes have important application value, and the purple transgenic tomatoes have certain ornamental value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to the application of the purple cabbage anthocyanin biosynthesis regulatory gene BrTT8 in cultivating light-induced purple tomatoes, and also relates to the application of cultivating light-induced purple tomatoes. Background technique [0002] As a bulk vegetable crop widely cultivated in the world, tomato is a popular vegetable and fruit. As a kind of pigment distributed in higher plants, flavonoids have been studied more and more. This kind of substances not only play a positive role in the response of plants to adverse environments (ultraviolet radiation, low temperature, drought and pathogenic bacteria invasion), but also have a positive effect on a large number of human beings. Treatment and recovery of chronic diseases (cardiovascular disease and tumors) are helpful. A large number of data confirm that regular consumption of foods rich in flavonoids has important val...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82A01H5/00
CPCC07K14/415C12N15/8205C12N15/8261
Inventor 胡宗利张彦杰陈国平高琼朱智国于晓惠
Owner CHONGQING UNIV
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