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Application of natural eutectic solvent in selective extraction of anthocyanin and preservation of anthocyanin

A deep eutectic solvent and anthocyanin technology, which is applied in the field of food science and engineering, can solve the problems of poor stability of anthocyanins, affecting the physiological activity of color rendering, loss of anthocyanins, etc., to achieve protection stability and improve stability , the effect of inhibiting degradation

Active Publication Date: 2021-03-16
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, due to the large variety of hydrogen bond donors and hydrogen bond acceptor molecules in natural deep eutectic solvents, solvents with different polarity, solubility and other physical and chemical properties can be customized through different hydrogen bond donor-acceptor combination schemes; At the same time, the stability of anthocyanins is relatively poor, and they are easily induced by various factors in the environment (such as temperature, pH, oxygen, light, etc.) to undergo chemical changes, thereby affecting their coloring performance and physiological activity.
Ultrasonic-assisted extraction is a technique commonly used in the extraction of anthocyanins. However, it is difficult to solve the problem of local high temperature overheating near the ultrasonic probe, so a large amount of anthocyanins are also lost during the extraction process.

Method used

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  • Application of natural eutectic solvent in selective extraction of anthocyanin and preservation of anthocyanin
  • Application of natural eutectic solvent in selective extraction of anthocyanin and preservation of anthocyanin
  • Application of natural eutectic solvent in selective extraction of anthocyanin and preservation of anthocyanin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] This example provides a method for simultaneously extracting delphinidin-3-galactoside / arabinoside and mallow pigment-3-arabinoside with strong selectivity from blueberry pomace, while protecting its stability method, comprising the steps of:

[0036] Step (1): Freeze the freshly squeezed blueberry pomace, and pre-freeze at -20°C for 24 hours, then vacuum freeze-dry in a large freeze dryer for 36 hours, crush and grind, and avoid light during the whole process;

[0037] Step (2): Use choline chloride as the hydrogen bond acceptor and oxalic acid as the hydrogen bond donor. The molar ratio of the two is 2:1. It needs to be heated in a water bath at 80°C and kept stirring to avoid local denaturation and obtain clarification After transparent and homogeneous liquid (natural deep eutectic solvent), the amount of water added is 30% (w / w, the mass ratio of water to natural deep eutectic solvent), mixed with the sample powder in step (1), the liquid-solid ratio It is 50mL / g, ...

Embodiment 2

[0041]This example provides a method for simultaneously extracting delphinidin-3-galactoside / arabinoside and mallow pigment-3-arabinoside with strong selectivity from blueberry pomace, while protecting its stability The technical solution includes the following steps:

[0042] Step (1): Freeze the freshly squeezed pomace, and pre-freeze at -40°C for 24 hours, then vacuum freeze-dry in a large freeze dryer for 36 hours, crush and grind, and avoid light during the whole process;

[0043] Step (2): Use choline chloride as the hydrogen bond acceptor and oxalic acid as the hydrogen bond donor with a molar ratio of 1:1. It needs to be heated in a water bath at 80°C and kept stirring to avoid local denaturation and obtain a clear and transparent After the homogeneous liquid, add 30% (w / w) of water, mix with the sample powder in step (1), the liquid-solid ratio is 60mL / g, preheat at 45°C for 5min, make the sample powder in the natural eutectic Uniform diffusion in the solvent;

[00...

Embodiment 3

[0047] This example provides a method for simultaneously extracting delphinidin-3-galactoside / arabinoside and mallow pigment-3-arabinoside with strong selectivity from blueberry pomace, while protecting its stability The technical solution includes the following steps:

[0048] Step (1): Freeze the freshly squeezed pomace, and pre-freeze at -60°C for 24 hours, then vacuum freeze-dry in a large freeze dryer for 24 hours, crush and grind, and avoid light during the whole process;

[0049] Step (2): Use choline chloride as the hydrogen bond acceptor and oxalic acid as the hydrogen bond donor with a molar ratio of 1:2. It needs to be heated in a water bath at 80°C with constant stirring to avoid local denaturation and obtain a clear and transparent After a uniform liquid, add 30% (w / w) of water, mix with the sample powder in step (1), the liquid-solid ratio is 60mL / g, preheat at 40°C for 8min, and make the sample powder in the natural eutectic Uniform diffusion in the solvent;

...

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Abstract

The invention discloses an application of a natural eutectic solvent in selective extraction of anthocyanin, which comprises the following steps: an aqueous solution of the natural eutectic solvent isuniformly mixed with blueberry pomace powder, and an anthocyanin crude extract is extracted under ultrasonic and water bath heating conditions; the natural eutectic solvent takes choline chloride asa hydrogen bond acceptor and oxalic acid as a hydrogen bond donor; wherein the molar ratio of choline chloride to oxalic acid is 1: 0.5-2; wherein in the aqueous solution of the natural eutectic solvent, the mass ratio of water to the natural eutectic solvent is 10-50%; the anthocyanin with delphinidin-3-galactoside, delphinidin-3-arabinoside and mallow pigment-3-arabinoside as main components canbe obtained from the anthocyanin crude extract. The invention also discloses an application of the natural eutectic solvent in anthocyanin preservation. The eutectic solvent provided by the inventionhas strong selectivity on delphinidin-3-galactoside, delphinidin-3-arabinoside and mallow pigment-3-arabinoside, and can effectively improve the stability of delphinidin-3-galactoside, delphinidin-3-arabinoside and mallow pigment-3-arabinoside.

Description

technical field [0001] The invention relates to the technical field of food science and engineering, in particular to the application of a natural deep eutectic solvent in selective extraction and preservation of anthocyanins. Background technique [0002] Anthocyanins belong to flavonoids and are widely found in fruits, vegetables and miscellaneous grains that people consume daily. According to the type and conditions of specific anthocyanins, anthocyanins can present a variety of colors, and are safe and non-toxic. They also have anti-oxidation, anti-inflammatory, anti-cancer, hypoglycemic, hypolipidemic, and protection of internal organs from oxidative stress damage, Inhibits a wide range of physiological activities such as obesity. In recent years, it has been used more and more in the field of food processing to replace traditional artificial synthetic pigments. Anthocyanins from some sources have been included in the national standards of food additives in China (GB2...

Claims

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Application Information

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IPC IPC(8): C09B61/00C09B67/54C07H17/065C07H1/08
CPCC07H1/08C07H17/065C09B61/00C09B67/0096
Inventor 罗自生付熙哲邹礼根徐艳群李莉林星宇
Owner ZHEJIANG UNIV
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